Applied Biosystems Veriti, User Manual

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6-3 Veriti™ Thermal Cycler User Guide
Chapter 6 Maintenance
Decontaminating
the Sample Wells
If the sample wells become contaminated with any biological agents, clean the wells
thoroughly with a cotton swab soaked in 1:10 v/v dilution of 5.25% sodium
hypochlorite (Chlorox bleach).
Use bleach solution in moderation. Applied Biosystems recommends the use of
10% bleach solution for removing contamination from the Veriti™ instrument
sample block(s); excessive use of the solution, however, can corrode the sample
block(s) material. To prevent damage to the sample block(s):
• Avoid applying excessive amounts of bleach solution. If possible, instead of
using a squeeze bottle or soaked cloth, use an atomizer to deliver the solution to
the wells of the block(s).
• After treating with bleach solution, rinse the sample block(s) thoroughly using
deionized water.
Note: Removing residual bleach from the surfaces of the Veriti™ instrument using
water minimizes the long-term effects of bleach treatments.
If the sample block(s) become contaminated with radioactivity, use a commercially
available decontaminant to remove the contamination. If the block(s) cannot be
decontaminated, the instrument cannot be returned to Applied Biosystems for
service.
Cleaning the
Heated Cover
Clean the heated cover once a month or as needed.
During instrument operation, the temperature of the heated cover
can be as high as 108 °C, and the temperature of the sample block(s) can be as high
as 100 °C. Before performing the procedure, keep hands away until the heated cover
and sample block(s) reach room temperature.
To clean the heated cover:
1. Follow the steps in “Preparation” on page 6-2 .
2. Open the heated cover.
3. Soak a cotton swab or piece of clean cloth with pure isopropanol and gently
wipe the heated platen.
CHEMICAL HAZARD . Isopropanol is a flammable
liquid and vapor. Exposure may cause eye, skin, and upper respiratory tract
irritation. Prolonged or repeated contact may dry skin and cause irritation.
Exposure may cause central nervous system effects such as drowsiness,
dizziness, and headache. Read the MSDS, and follow the handling instructions.
4. Remove any remaining isopropanol from the cover.
Note: If the platen becomes contaminated with amplified DNA, then raise the
heated cover to the cleaning position, wipe the platen with a cloth or cotton
swab soaked in bleach, then rinse with water.
Clean the heated platen once a month or as needed.