Analytik Jena aj CYBERTRON InnuPure C12 Manual Download Page 14 | Manualshive

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Device description and functional principle

Functional principle

12

Issue 01/2011

InnuPure C12

Elution

The last tube contains the Elution Buffer. The magnetic particles are left inside

the tube, the nucleic acid is eluted in a defined volume and the magnetic

particles are removed again from the sample.
The nucleic acid is now purified, pipetted to the final Elution Tube and ready for

other down-stream applications.

Fig. 3-9

Elution of DNA/RNA

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Summary of Contents for aj CYBERTRON InnuPure C12

Page 1: ...InnuPure C12 Extraction system...

Page 2: ...formation about Analytik Jena AG on the internet http www bio analytik jena de Copyrights and Trademarks Microsoft Windows 2000 XP Vista MS Excel are registered trademarks of Microsoft Corp Publicatio...

Page 3: ...own 15 5 2 Assembly instruction Priming Station 16 5 3 Preparation of buffers and solutions 16 5 4 Preparation of the sample tray 17 5 5 Position of the sample tray 18 6 Packaging instruction 19 7 Ope...

Page 4: ...tion protocols from InnuPure C12 33 8 8 Transfer of isolation protocols 33 8 8 1 Copy isolation protocols 34 8 8 2 Move isolation protocols 34 8 9 Up load of isolation protocols to InnuPure C12 34 8 1...

Page 5: ...cleic acids to be isolated are adsorbed to surface functionalized magnetic particles The required extraction chemistry is optimally adapted to the application at hand and facilitates the isolation of...

Page 6: ...marked by square brackets e g Save Cross reference to other sections and illustrations italic Note Observe the notes marked in this way to ensure correct function of the device and to avoid operating...

Page 7: ...valid regulations This Symbol is on the rear side of device 2 2 General warning information Proper use The InnuPure C12 including its original accessories may only be used for the applications descri...

Page 8: ...ay be used Caution condensed water In case of large differences between the storage and installation temperatures before connecting wait until the InnuPure C12 has attained the ambient temperature to...

Page 9: ...ice Ensure that the ventilation slits on the InnuPure C12 are kept free at all times Water Ensure that no liquid can enter the InnuPure C12 The device could be damaged Do not place any containers with...

Page 10: ...adapter 4 Sample tray Fig 3 2 View inside of InnuPure C12 Via the admission 6 in Fig 3 1 of the sample tray 5 in Fig 3 1 in the front door 4 in Fig 3 1 all necessary pre filled strips containing all b...

Page 11: ...section Up date of the InnuPure C12 on page 29 Therefore a PC is needed A green and a red LED status indicators 1 in Fig 3 1 are mounted beside the display 2 of the InnuPure C12 to show the active an...

Page 12: ...performed manually outside of the device The lysis routine for each starting material is described in detail in the manual of the extraction kit Lysis outside the device Lysis inside the device Fig 3...

Page 13: ...etely by the plastic cover Fig 3 6 Sample processing Washing The magnetic particles with the bound nucleic acid are moved to the next tubes By up and down pipetting of different washing buffers the nu...

Page 14: ...tains the Elution Buffer The magnetic particles are left inside the tube the nucleic acid is eluted in a defined volume and the magnetic particles are removed again from the sample The nucleic acid is...

Page 15: ...g motors Avoid water drips splashes and spray close to the InnuPure C12 Do not expose the InnuPure C12 to direct sunlight or radiation from heaters Temperature range in operation 5 40 C Humidity in o...

Page 16: ...r storage and transport The following environment conditions must be observed for transport and storage Temperature range 40 70 C Humidity up to 80 Note Before transportation and storage of the device...

Page 17: ...nd installation temperatures wait until the InnuPure has attained the ambient temperature before connecting to avoid damage to the device caused by condensed water 1 Remove the InnuPure C12 from its p...

Page 18: ...utions All buffers and solutions are provided in pre filled and sealed strips regarding to the isolation routine and starting material The preparation of sample tray is described in detail in each Kit...

Page 19: ...sample tray Before an extraction process can be started the tray has to be pre loaded with additional equipment as shown below or described in detail in the user manual of each special purification i...

Page 20: ...he Reagent Strips MFR filter tips Elution Tubes is depending on the application please refer to the description in the user manual of the kit 5 5 Position of the sample tray The pre loaded sample tray...

Page 21: ...ollow this packaging instruction strictly to avoid any damages at the device during the transportation Attention Before packaging all samples reagents and the sample tray have to be removed from the d...

Page 22: ...C12 Impose the covering box without lid and bottom on the InnuPure C12 Put the foamed plastic insert onto the device between the bars of the second foamed plastic Attention Make sure that the coverin...

Page 23: ...p of the waste box Cover the parts with burling foam sheets Place the foamed plastic lid with the user manual and CD onto the foamed plastic insert Place the lid of the packaging onto the covering box...

Page 24: ...e C12 until perceiving a slight resistance On pressing against this resistance the tray will be drawn in automatically 5 Choose and start the isolation protocol Performance of an isolation routine p 2...

Page 25: ...d in the following 1 Switch on the InnuPure C12 on the rear panel of the device Construction and connections p 8 The device is now initialized automatically 2 Wait until a beep is audible and the star...

Page 26: ...ter the initialization is finished the Tools are displayed again 7 3 2 Waste tips ENTER Start 1 Use cursor down to select Tools inside the main menu and confirm with pressing ENTER Start 2 The Tools s...

Page 27: ...m with pressing ENTER Start 3 During the progress the shown screen appears the tray is moved out of device 4 After the tray was moved out the Tools are displayed again Note Pressing against the tray a...

Page 28: ...on kit carefully to find the right purification routine in dependence on the starting material After switching on the InnuPure C12 is initialized automatically Wait until a beep is audible before star...

Page 29: ...is finished the message program finished appears and the tray with the purified samples will be moved out automatically Additional a 3times peep is audible ENTER Start 6 Confirm with ENTER Start and...

Page 30: ...ing program 28 Issue 01 2011 InnuPure C12 4 The tips are wasted into the waste box the sample tray is moved out automatically and the shown screen appears ENTER Start 5 Confirm with ENTER Start and th...

Page 31: ...1 2 Installation procedure UpDateIP is delivered on a CD 6 Start your PC and Windows 7 Insert the CD into the CD drive e g D Trigger UpDateIP exe on the CD Optional it is possible to copy the software...

Page 32: ...on the menu command The language setting will change according to the most recently enabled command option 8 4 Help UpDateIP includes a context sensitive help tool Select the help window by clicking...

Page 33: ...ce includes necessary elements for up dates of isolation protocols on the InnuPure C12 Table to show protocols saved on the PC Table to show protocols loaded on the device Operation buttons in the mid...

Page 34: ...often are located as function buttons at the middle of the main window or in the toolbar at the upper window margin below to the main menu Load Loading of a new isolation protocols into the main windo...

Page 35: ...oad the isolation protocols from the InnuPure C12 During the download process all buttons in the up date tool become inactive 2 The isolation protocols which are saved on the device are shown in the D...

Page 36: ...Up load of isolation protocols to InnuPure C12 The option Upload of the InnuPure C12 has to be used to save all isolation protocols which are shown in the main window list Device protocols on the inte...

Page 37: ...moved from the protocol lists not from the PC If isolation protocols should be removed completely follow the windows routine for deleting data files and delete the protocols inside the accordant folde...

Page 38: ...Up date of the InnuPure C12 Delete isolation protocols 36 Issue 01 2011 InnuPure C12...

Page 39: ...r paper towel If InnuPure C12 is used for the analysis of infectious material special care must be taken as the InnuPure C12 cannot be decontaminated as a complete unit Visible soiling must be removed...

Page 40: ...g the fuses If the fuses in the InnuPure C12 are defective they can be replaced 1 Switch off the InnuPure C12 and disconnect the mains plug from the connector on the InnuPure C12 2 Open the fuse holde...

Page 41: ...st dispose of the waste materials arising from measurement sample materials in accordance with the legal and local regulations The InnuPure C12 with its electronic components is to be disposed of as e...

Page 42: ...Waste disposal 40 Issue 01 2011 InnuPure C12...

Page 43: ...to 12 samples in parallel Processing of different starting materials possible if pipetting protocols are identical Tube Volumes 0 5 2 ml for samples and buffer Number of tips Max 12 or 24 one or two...

Page 44: ...Technical data 42 Issue 01 2011 InnuPure C12...

Page 45: ...ns on the back side of the InnuPure C12 9 Fig 3 4 Keypad functions 9 Fig 3 5 Lysis of the starting material 10 Fig 3 6 Sample processing 11 Fig 3 7 Washing of the nucleic acids 11 Fig 3 8 Removing of...

Page 46: ...Table of figures 44 Issue 01 2011 InnuPure C12...

Page 47: ......

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