Agilent InfinityLab LC Series RID User Manual
68
4
Preparing the Module
Setting up an Analysis
Typical Chromatogram
A typical chromatogram for this analysis is shown in
on page 68. The
exact profile of the chromatogram will depend on the chromatographic
conditions. Variations in solvent quality, column packing, standard concentration
and column temperature will all have a potential effect on peak retention and
response.
Figure 16
Isocratic standard sample chromatogram
NOTE
The resulting chromatogram should only be seen as a qualitative example, the
checkout procedure is not meant as a quantitative procedure. Its intent is only to
verify the presence of the four peaks from the checkout sample.
Please be aware of the large negative air / solvent peak from the injection (cut
out from the bottom of the following figure) prior to the first peak of interest. This
is to be expected in a regular chromatogram, especially if a non-degassed
sample is injected into degassed solvent and the sample solvent properties don’t
perfectly match with the mobile phase properties. Only similar zooming factors
in the display of a chromatogram will lead to similar looking results.
