13 – LSM 880 Training Guide
Acquisition Setup
Once our light path setup has been completed we can expand the scanning tools to begin collecting an image. From
‘Acquisition Parameter’ there are 2 blue tabs used for imaging, ‘Acquisition Mode’ and ‘Channels’.
Acquisition Mode
This dialog controls static image settings such as:
Objective
– displays currently selected objective as programmed into
microscope.
Frame Size
– image size in pixels. Default is 512x512, however this can be
optimized based on objective and zoom settings. Ideal sampling
frequency can be quickly selected with the ‘Optimal’ button.
Scan Speed
– overall speed of the scan during acquisition. Reports pixel
dwell time and total scan time. Increasing dwell time (slowing down the
scan) will improve signal-to-noise ratio.
Bit Depth
– the number of bits used to indicate the range of signal level in
the sample. The default A/D conversion is 16 bit. Change this from the
default 8 Bit to 16 Bit always!
Averaging
– selecting ‘Number’ >1 will scan each X line the ‘number’ of
times and average the result. Use to reduce image noise.
Scan Area
– allows scan area adjustments such as X,Y displacement and
rotation of scan in 360 degrees. ‘Zoom’ can be used to increase
magnification without signal loss or objective change.