12 – LSM 880 Training Guide
Light Path Configuration
7.
Choose detector for imaging.
Note: PMT selection will be based on where your fluorophore(s) fall in the linear
range of the emission spectrum. Meaning that Ch1 typically corresponds to the
lowest wavelength while Ch2 corresponds to the longest wavelength fluorophore.
In our example, we will be using a sequential scan with Alexa 568 and Alexa 488, so
Ch1(or ChS1) will be used for the Alexa 488 signal and Ch2 will collect the Alexa
568.
8.
Define upper and lower emission band thresholds based on the spectrum of your
fluorophore.
9.
The system is equipped with a Transmitted Light Detector for imaging DIC or
brightfield images. Check to enable.
10.
Click + button to add another track. Repeat steps 3-8 for the next fluorophore
(Alexa 488 in this case).
