26 – LSM 880 Training Guide
Collecting a Tile Scan
To collect tiled images for fields of view larger than a single frame, we need to first activate the Tile Scan tools in ZEN which are
hidden by default.
1.
Check ‘Tile Scan’ from the experiment options. This will activate the Tile
Scan dialog in the Multidimensional Acquisition settings.
The Tile Scan dialog has 3 modes:
Centered grid
– creates a tile scan around the current stage position.
Here you can manually define the number of tiles in X and Y along with
the Overlap % to define the tile area.
Bounding grid
– creates a tile scan by defining the boundaries of the
section.
Convex hull
– similar to ‘Bounding grid’, this tool allows the user to define
the boundaries of the sample, but along a contour rather than a box.
2.
To collect a tile scan using ‘Centered grid’, position your sample so the
current position is located in the center of what will be the final tile.
3.
Define the total number of Horizontal and Vertical tiles.
4.
Select Overlap equal to at least 10%, ideally 20%.
5.
Click ‘Start Experiment’.
