7 – LSM 880 Training Guide
Mounting Sample on the Microscope
While the lasers are warming up, we can mount our specimen on the stage above the microscope objective.
1.
Place sample on microscope stage above objective (roughly in the
center of the field of view).
2.
Select the ‘Locate’ tab in the Image Acquisition Tools.
3.
Use the Shortcut buttons to select filter set for visualization in the
eyepieces.
Green
– generic green filter set
Red
– generic red filter set
Blue
– generic blue filter set
TL/DIC
– transmitted light
All Closed
– turn off all light sources.
Note: These shortcut buttons will automatically select the filter set and
open the light source shutter.
Alternatively, you can turn on the epi-fluorescence light path manually
using the ‘Microscope Control’ tab located below the configuration
shortcuts. Here you can turn on/off the epi-fluorescence light source (A),
change your filter set (B) and control transmitted light (C) if applicable.