17 – LSM 880 Training Guide
Fine Tuning Image Quality
To fine tune the image intensity to ideal levels for final image acquisition:
1.
Select one Track at a time – begin by highlighting the first Track in your list.
Note: Make sure to uncheck all other tracks during this process so you are only
scanning one color at a time. This will save time and ultimately prevent any
unnecessary photobleaching of other channels.
2.
Click ‘Live’ to start scanning.
3.
Turn on ‘Range Indicator’ LUT as shown on the previous page.
4.
Deselect laser checkbox for the active track.
5.
Adjust Digital Offset until background areas are just above the display of any
blue
pixels.
6.
While scanning, reselect the laser line (4) and increase Gain (Master) until the
brightest areas in the image are just below the display of any
red
pixels.
7.
Stop Scan.
8.
Repeat this process for each track independently.
9.
Click ‘Snap’ to collect final image.
