Zeiss Cell Observer spinning disk confocal Abridged Instructions Download | Manualshive

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Wellcome Trust Centre for Human Genetics

Cellular-Imaging Microscopy Core

The Zeiss Spinning Disk
Confocal

Abridged INSTRUCTIONS

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Summary of Contents for Cell Observer spinning disk confocal

Page 1: ...Page 1 Wellcome Trust Centre for Human Genetics Cellular Imaging Microscopy Core The Zeiss Spinning Disk Confocal Abridged INSTRUCTIONS...

Page 2: ...g Disk Unit Photometrics Evolve 512 Delta EM CCD camera 512 x 512 pixels Applied Scientific MS2000 Piezo motorised XY stage Applied Scientific fast galvo Z focus 150um range Definite Focus with NIR LE...

Page 3: ...leave Zen so save them first In event of Zen crashing or PC mains failure the files will remain in Temp Use snap in Zen Blue to clear noise from the camera detectors before imaging Setting snap for e...

Page 4: ...g too much It is far easier to control the DAPI GFP and DsRed mercury lamp fluorescence as viewed down the eyepieces from the PC s Zen Blue software Alcohol 70 is provided along with gloves and green...

Page 5: ...pressing its rear rocker switch to the right the middle yellow heating on LED will light up To set the microscope stage incubator temperature press Home Microscope and Incubation on the microscope s L...

Page 6: ...e LED should mark nom Press the and buttons to set the nominal value To view the CO2 real value press the button display again Regulate the CO2 influx with the CO2 reducing valve The valve should be a...

Page 7: ...into the Windows 7 desktop switch on the large Evolve Delta 512x512 EMCCD camera 7 Start Zen Blue 8 Select Zen System Start System 9 Place the specimen onto the microscope 10 Use the DAPI GFP RFP and...

Page 8: ...o the Load lowest position using the microscope coarse focus control 5 Transfer image files to the network or data key 6 Shut Down the PC workstation 7 Switch off the large Evolve Delta 512x512 EMCCD...

Page 9: ...any microscope imaging problems or queries just ask a member of the Microscopy Core staff for help There is additional help and advice on our Core web pages http www well ox ac uk microscopy WTCHG Ze...

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