74
PE, PC and Chlorophyll Calibration 2-Point
Each channel of the sensor must be calibrated independently. Calibration of
the chlorophyll channel does not set the calibration for the PC channel or the
PE channel. In addition, calibrating in RFU for a channel does not automatically
calibrate the µg/L measurement for the same channel. The following calibration
procedure must be performed for each channel and each unit the user would like
to display.
1.
Fill the graduated cylinder to the appropriate level with deionized water (0
standard). Immerse the probe in the standard. Make sure the sensor guard
is installed.
2.
Push the
Cal
key, then select either
TAL-PC
or
TAL-PE
, depending on the
sensor to be calibrated.
3.
Select the channel and units to be calibrated. Options for the TAL-PC and
the TAL-PE sensors are shown in the figures on the left.
4.
Select
Calibration Value
and enter 0.00.
5.
Make sure there are no air bubbles on the sensor lens. If present, lightly
tap the guard against the cup to dislodge any bubbles. Observe the
actual measurement readings for stability (white line on graph shows no
significant change for 40 seconds), and then select
Accept Calibration
.
“Ready for cal point 2” will be displayed in the message area.
6.
Discard the used water, and rinse the probe, guard, and graduated
cylinder with a small amount of the standard for calibration point #2.
Discard the rinse standard.
NOTE:
For standard #2, use the 0.625 mg/L Rhodamine WT solution when
calibrating chlorophyll (RFU or µg/L) on either TAL sensor, or when
completing a PC (RFU or µg/L) calibration on a TAL-PC sensor. Use
the 0.025 mg/L Rhodamine WT solution when completing a PE
(RFU or µg/L) calibration on a TAL-PE sensor.
7.
Fill the graduated cylinder to the appropriate level with fresh standard #2.
Immerse the sensors in the second calibration standard.
8.
Observe the temperature reading on the calibration display. Use the table
in the
Rhodamine WT dye solution preparation section
to identify the
appropriate value for the calibration standard.
9.
Select
Calibration Value
and enter the value of the second calibration
standard.
10.
Observe the actual measurement readings for stability (white line on
graph shows no significant change for 40 seconds), then select
Accept
Calibration
. The procedure will automatically finish after calibrating using
the second standard.
Sensors, Calibration, and Maintenance