4-4
Maintaining the Detector
Routine maintenance
The detector requires minimal routine maintenance.
To achieve optimal performance,
•
replace solvent reservoir filters in your HPLC system regularly.
•
filter and degas solvents to prolong column life, reduce pressure
fluctuations, and decrease baseline noise.
•
flush buffered mobile phases out of the detector with HPLC-grade water
followed by a 5 to 10% methanol solution each time the detector is
powered-off. This process prevents
–
plugging of the solvent lines and flow cell.
–
damage to the components.
–
microbial growth.
Maintaining the flow cell
A dirty flow cell can cause baseline noise, decreased sample energy levels,
calibration failure, and other problems with operation of the detector.
There are two phases in cleaning the flow cell:
•
Flushing
•
Removing and cleaning
If flushing is ineffective, remove and clean the flow cell. Replace flow cell
components as required.
This section provides information about the following procedures:
•
Flushing the flow cell
•
Removing and cleaning the flow cell
•
Disassembling and reassembling the flow cell
Caution:
Always replace the flow cell gaskets when cleaning,
rebuilding, or replacing other flow cell components.
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