5
Protein Applications
Measure Protein A280
48 NanoDrop Eight User Guide
Thermo Scientific
Other protein
(
+ MW)
User entered molar
extinction coefficient
and molecular
weight
Assumes protein has known molar extinction
coefficient (
) and molecular weight (MW),
where:
(
molar
)*10=(
percent
)*(MW
protein
)
Enter MW in kiloDaltons (kDa) and molar
extinction coefficient (
)
in M
-1
cm
-1
divided by
1000 (i.e.,
/1000). For example, for protein
with molar extinction coefficient of 210,000
M
-1
cm
-1
, enter 210.
Other protein
(
1%)
User entered mass
extinction coefficient
Assumes protein has known mass extinction
coefficient (
). Enter mass extinction
coefficient in L/gm-cm for 10 mg/mL (
1%)
protein solution.
a
To add or edit a custom protein, use Protein Editor.
Setting
Available Options
Mass Ext.
Coefficient
(L/gm-cm)
Description
Setting
Available Options
Mass Ext.
Coefficient
(L/gm-cm)
Description
Baseline
Correction
On or off
Enter baseline
correction
wavelength in nm or
use default value
(340 nm)
N/A
Corrects for any offset caused by light
scattering particulates by subtracting
measured absorbance at specified baseline
correction wavelength from absorbance
values at all wavelengths in sample spectrum.
As a result, absorbance of sample spectrum is
zero at specified baseline correction
wavelength.
Tip
: If the sample has a modification that
absorbs light at 340 nm, select a different
correction wavelength or turn off Baseline
Correction.
Содержание NanoDrop Eight
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Страница 30: ...4 Nucleic Acid Applications 30 NanoDrop Eight User Guide Thermo Scientific This page is intentionally blank...
Страница 40: ...4 Nucleic Acid Applications Measure Oligo DNA or Oligo RNA 40 NanoDrop Eight User Guide Thermo Scientific...
Страница 56: ...5 Protein Applications Measure Protein A280 56 NanoDrop Eight User Guide Thermo Scientific...
Страница 76: ...6 Custom Applications Measure Custom 76 NanoDrop Eight User Guide Thermo Scientific...
Страница 126: ...7 Learning Center Measurement Screen Display Options 126 NanoDrop Eight User Guide Thermo Scientific...
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