ThermoFisher Scientific NanoDrop Eight, Руководство пользователя

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6
Custom Applications
Measure UV-Vis
Thermo Scientific NanoDrop Eight User Guide 59
4. Select Blank and wait for the measurement to complete.
Tip : If Auto-Blank is On, the blank measurement starts automatically after you
lower the arm.
5. Lift the arm and clean all pedestals with a new laboratory wipe, .
6. Pipette 1-2 µL sample solution onto the pedestal and lower the arm.
7. Start the sample measurement: If Auto-Measure is On, lower arm;
if Auto-Measure is off, lower arm and select Measure .
When the sample measurement is completed, the spectrum and reported values
are displayed (see the next section).
8. When you are finished measuring samples, select End Experiment .
9. Lift the arm and clean all pedestals with a new wipe.
Best practices for UV-Vis measurements
• Ensure the sample absorbance is within the instrument’s absorbance detection
limits .
• Blank with the same buffer solution used to re-suspend the analyte of interest.
The blanking solution should be a similar pH and ionic strength as the analyte
solution.
• Run a blanking cycle to assess the absorbance contribution of your buffer
solution. If the buffer exhibits strong absorbance at or near an analysis
wavelength, you may need to choose a different buffer or application. See
Choosing and Measuring a Blank for more information.
• For micro-volume measurements:
– Ensure pedestal surfaces are properly cleaned and conditioned.
– Ensure samples are homogeneous before taking a measurement. Avoid
introducing bubbles when mixing and pipetting.
– Follow best practices for micro-volume measurements.
– Use a 1-2 µL sample volume. See Recommended Sample Volumes for more
information.