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5

Protein Applications

Measure Protein A280

52 NanoDrop Eight User Guide

Thermo Scientific

The extinction coefficient of a peptide or
protein is related to its tryptophan (W),
tyrosin (Y) and cysteine (C) amino acid
composition.

Tip

: The extinction coefficient is

wavelength specific for each protein and
can be affected by buffer type, ionic
strength and pH.

Extinction Coefficients for Proteins

At 280 nm, the extinction coefficient is approximated by the
weighted sum of the 280 nm molar extinction coefficients of
the three constituent amino acids, as described in this
equation:



= (nW * 5500) + (nY * 1490) + (nC * 125)

where:



= molar extinction coefficient

n

= number of each amino acid residue

5500, 1490 and 125

= amino acid molar absorptivities at

280 nm

This application offers six options (shown at
right) for selecting an appropriate extinction
coefficient for each measured sample, to
be used in conjunction with Beer’s Law to
calculate sample concentration.

If the extinction coefficient of the sample is
known, choose the



+ MW (molar) or



1% (mass) option and enter the value.

Otherwise, calculate the extinction
coefficient or choose the option that best
matches the sample solution.

Tip

: Ideally, the extinction coefficient

should be determined empirically using a
solution of the study protein at a known
concentration using the same buffer.

Available Options for Extinction Coefficient

•

1 Abs = 1 mg/mL

, where sample type and/or ext.

coefficient is unknown (produces rough estimate of
protein concentration)

•

BSA

(Bovine Serum Albumin, 6.7 L/gm-cm)

•

IgG

(any mammalian antibody, 13.7 L/gm-cm)

•

Lysozyme

(egg white lysozyme, 26.4 L/gm-cm)

•

Other protein

(



+ MW), user-specified molar ext.

coefficient

•

Other protein

(



1%), user-specified mass ext.

coefficient

•

Note

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Содержание NanoDrop Eight

Страница 1: ...NanoDrop Micro UV Vis Spectrophotometer NanoDrop Eight User Guide M020 NanoDrop Eight UG Revision Date December 2021...

Страница 2: ...l technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fi...

Страница 3: ...se contact 12 Chapter 3 Application Measurement Ranges 13 Detection Limits for All Applications 13 Chapter 4 Nucleic Acid Applications 15 Measure dsDNA ssDNA or RNA 15 Measure dsDNA ssDNA or RNA 15 Be...

Страница 4: ...ein A280 Measurements 51 Calculations for Protein A280 Measurements 51 Chapter 6 Custom Applications 57 Measure UV Vis 58 Measure UV Vis 58 Best practices for UV Vis measurements 59 UV Vis Reported Re...

Страница 5: ...rement Screen Display Options 124 Chapter 8 Maintenance 127 Maintenance Schedule 128 Daily Maintenance 128 Periodic Maintenance 128 Every 6 Months 128 Maintaining the Pedestals 129 Cleaning the Pedest...

Страница 6: ...Contents vi NanoDrop Eight User Guide Thermo Scientific...

Страница 7: ...thout the need for dilutions The instrument accurately measures up to 8 individual 1 l samples in one measurement cycle The software allows the user to measure samples using either the full 8 channel...

Страница 8: ...r features the patented micro volume sample retention system USB B port Connect the NanoDrop Eight to your PC via USB to operate the instrument using the PC software Note Before operating a NanoDrop E...

Страница 9: ...aining plastic consumables and liquid photometric standard used to check instrument performance For more information see Performance Verification Specially formulated conditioning compound that can be...

Страница 10: ...e Spectrophotometer Instrument Detection Limits 10 NanoDrop Eight User Guide Thermo Scientific Instrument Detection Limits Pathlength mm Upper Detection Limit 10 mm Equivalent Absorbance 1 0 12 5 0 2...

Страница 11: ...com nanodropsw On the website locate the Register Your Instrument button and follow the instructions to register the instrument Computer Requirements Required Windows Operating System Windows 10 Enter...

Страница 12: ...llation folder downloaded from the internet 2 Launch Start exe and click the Software button The software installer will run Technical Support For U S Canada Support please contact Thermo Fisher Scien...

Страница 13: ...f 0 04 200 A Sample Type Lower Detection Limit Upper Detection Limit Typical Reproducibilitya a Based on five replicates SD ng L CV dsDNA 2 0 ng L 10 000 ng L 2 0 ng L for sample concentrations betwee...

Страница 14: ...3 Application Measurement Ranges 14 NanoDrop Eight User Guide Thermo Scientific This page is intentionally blank...

Страница 15: ...A230 A single point baseline correction can also be used To measure dsDNA ssDNA or RNA samples Measures the concentration of purified dsDNA ssDNA or RNA samples that absorb at 260 nm Measure dsDNA ssD...

Страница 16: ...ify a baseline correction if desired 3 Pipette 1 2 L blanking solution onto the lower pedestal and lower the arm 4 Select Blank and wait for the measurement to complete Tip If Auto Blank is On the bla...

Страница 17: ...s Isolate and purify nucleic acid samples before measurement to remove impurities Depending on the sample impurities could include DNA RNA free nucleotides proteins some buffer components and dyes See...

Страница 18: ...ring a Blank for more information For micro volume measurements Ensure pedestal surfaces are properly cleaned and conditioned If possible heat highly concentrated or large molecule samples such as gen...

Страница 19: ...trument software Click row to select sample and update spectrum UV spectrum Right click graph area to view display options Measure sample End experiment Run Blank Purity ratios Menu of table options c...

Страница 20: ...minant corrected Settings for Nucleic Acid Measurements Baseline Correction For the dsDNA ssDNA or RNA enter baseline correction wavelength in nm or use default value 340 nm Optional user defined base...

Страница 21: ...factor f 1 b where wavelength dependent molar extinction coefficient in ng cm L b sample pathlength in cm As a result analyte concentration c is calculated as c A 1 b or c A f where c analyte concent...

Страница 22: ...on Measured Values Note For micro volume absorbance measurements the spectra are normalized to a 10 mm pathlength equivalent A260 absorbance Nucleic acid absorbance values are measured at 260 nm using...

Страница 23: ...orbance at 230 nm An A260 A230 purity ratio between 1 8 and 2 2 is generally accepted as pure for DNA and RNA Note Although purity ratios are important indicators of sample quality the best quality in...

Страница 24: ......

Страница 25: ...tal measurements with the NanoDrop Eight instrument lift the instrument arm and clean the upper and lower pedestals At a minimum wipe the pedestals with a new laboratory wipe For more information see...

Страница 26: ...k measurement starts automatically after you lower the arm 5 Lift the arm and clean all pedestals with a new laboratory wipe 6 Pipette 1 2 L sample solution onto the pedestals and lower the arm 7 Star...

Страница 27: ...oom Right click and select Autoscale to fit spectra to window Measure sample solution Select to toggle Auto Measure ON OFF Sample names select to add or edit Run blank Back to Home screen Tips Click s...

Страница 28: ...aw equation Based on extinction coefficient and pathlength f 1 260 b where f factor molar extinction coefficient at 260 nm in ng cm L b sample pathlength in cm 1 cm for nucleic acids measured with the...

Страница 29: ...imits are dependent on the upper absorbance limit of the instrument and the user defined extinction coefficients To calculate upper detection limits for nucleic acid samples To calculate upper detecti...

Страница 30: ...4 Nucleic Acid Applications 30 NanoDrop Eight User Guide Thermo Scientific This page is intentionally blank...

Страница 31: ...anoDrop Eight instrument lift the instrument arm and clean the upper and lower pedestals At a minimum wipe the pedestals with a new laboratory wipe For more information see Cleaning the Pedestals To m...

Страница 32: ...ift the arm and clean all pedestals with a new laboratory wipe 6 Pipette 1 2 L sample solution onto the pedestal and lower the arm 7 Start the sample measurement If Auto Measure is On lower arm if Aut...

Страница 33: ...ed results Here is an example Oligo DNA and Oligo RNA reported values Nucleic acid concentration Click row to select sample and update spectrum click more rows to overlay spectra UV spectrum Purity ra...

Страница 34: ...ate sample measurement was taken nucleic acid concentration A260 A280 A260 A230 A260 A280 factor oligo sequence baseline correction Related Topics Basic Instrument Operations Oligo Calculations Note T...

Страница 35: ...ence for RNA Use the G A U and C keys to specify the RNA base sequence Specify your DNA or RNA base sequence click the corresponding keys You can also enter base sequence using the keyboard or by copy...

Страница 36: ...ng L Molecular Weight of oligo calculated from user defined base sequence Number of Bases entered Molar Ext Coefficient 260 nm Molar extinction coefficient of oligo in ng cm L at 260 nm calculated fro...

Страница 37: ...sentially randomized sequences but not for short synthetic oligo sequences To ensure the most accurate results we use the exact value of 260 to calculate oligonucleotide concentration The NanoDrop sof...

Страница 38: ...A260 absorbance Note For micro volume absorbance measurements and measurements taken with nonstandard other than 10 mm cuvettes the spectra are normalized to a 10 mm pathlength equivalent Nucleic aci...

Страница 39: ...cally have a higher 260 280 ratio due to the higher ratio of Uracil compared to that of Thymine Reported Values Nucleic acid concentration Reported in selected unit i e ng L g l or g mL Calculations a...

Страница 40: ...4 Nucleic Acid Applications Measure Oligo DNA or Oligo RNA 40 NanoDrop Eight User Guide Thermo Scientific...

Страница 41: ...and one absorbance ratio A260 A280 A single point baseline correction can also be used This application does not require a standard curve To measure Protein A280 samples Measures the concentration of...

Страница 42: ...desired 3 Pipette 1 2 L blanking solution onto the lower pedestal and lower the arm 4 Click Blank and wait for the measurement to complete Tip If Auto Blank is On the blank measurement starts automat...

Страница 43: ...on the sample impurities could include DNA RNA and some buffer components See Preparing Samples for more information Ensure the sample absorbance is within the instrument s absorbance detection limit...

Страница 44: ...r or application See Choosing and Measuring a Blank for more information For micro volume measurements Ensure pedestal surfaces are properly cleaned and conditioned Proteins tend to stick to pedestal...

Страница 45: ...ine correction wavelength Click row to select sample and update spectrum UV spectrum Right click graph area to view display options Measure sample End experiment Run Blank Purity ratios Menu of table...

Страница 46: ...select the settings icon to view Protein A280 Setup Protein A280 settings The Protein A280 application provides a variety of sample type options for purified protein analysis Each sample type applies...

Страница 47: ...lculates BSA Bovine Serum Albumin protein concentration using mass extinction coefficient of 6 7 L gm cm at 280 nm for 1 i e 10 mg mL BSA solution Assuming MW is 66 400 daltons Da molar extinction coe...

Страница 48: ...inction coefficient in L gm cm for 10 mg mL 1 protein solution a To add or edit a custom protein use Protein Editor Setting Available Options Mass Ext Coefficient L gm cm Description Setting Available...

Страница 49: ...Protein Editor select to show the New Protein Type box 2 Enter a unique Name for the new protein 3 Enter a Description for the new protein 4 Specify whether to enter Molar Extinction coefficient or Ma...

Страница 50: ...loDaltons kDa 5 Select Save to close the New Protein Type box The new custom protein appears in the Type list in Protein A280 Setup and Proteins Labels Setup Edit custom protein 1 In Protein Editor cl...

Страница 51: ...10 For example if the sample s mass extinction coefficient at 280 nm is 6 7 for a 1 10 mg mL solution the equation looks like this 200 6 7 10 298 5 or 300 Calculations for Protein A280 Measurements T...

Страница 52: ...shown at right for selecting an appropriate extinction coefficient for each measured sample to be used in conjunction with Beer s Law to calculate sample concentration If the extinction coefficient o...

Страница 53: ...ctly The equation at the right shows the relationship between molar extinction coefficient molar and percent extinction coefficient 1 Conversions Between molar and 1 molar 10 1 MWprotein Example To de...

Страница 54: ...sample such as residual solvents and reagents typically used during sample purification Measured Values A280 absorbance Note For micro volume absorbance measurements the spectra are normalized to a 1...

Страница 55: ...e at 260 nm to corrected absorbance at 280 nm An A260 A280 purity ratio of 0 57 is generally accepted as pure for proteins Note Although purity ratios are important indicators of sample quality the be...

Страница 56: ...5 Protein Applications Measure Protein A280 56 NanoDrop Eight User Guide Thermo Scientific...

Страница 57: ...s Use the NanoDrop Eight to perform UV Vis measurements The UV Vis application allows the instrument to function as a conventional spectrophotometer Up to 40 wavelengths from 190 nm to 850 nm can be m...

Страница 58: ...new laboratory wipe For more information see Cleaning the Pedestals To measure a sample using the UV Vis application 1 From the Home screen from the Custom tab select UV Vis 2 Specify up to 40 wavelen...

Страница 59: ...w wipe Best practices for UV Vis measurements Ensure the sample absorbance is within the instrument s absorbance detection limits Blank with the same buffer solution used to re suspend the analyte of...

Страница 60: ...o Home screen Tips Click sample row to select sample and update spectrum Shift click multiple sample rows to overlay spectra Click a sample and hover locations on spectra to view measurement values Sa...

Страница 61: ...sample row to select sample and update spectrum Shift click multiple sample rows to overlay spectra Click a sample and hover locations on spectra to view measurement values Sample rows Select to add...

Страница 62: ...nm This is the wavelength the software will use to determine the pathlength selection Automated Pathlength On or Off affects pedestal measurements only Optional automated pathlength selection Allows t...

Страница 63: ...in Before taking measurements with the NanoDrop Eight instrument lift the instrument arm and clean the upper and lower pedestals At a minimum wipe the pedestals with a new laboratory wipe For more inf...

Страница 64: ...lect Custom Methods 2 In the method selection pane select the method to run Information about the selected method appears in the method details pane 3 Select 4 Follow the on screen instructions to mea...

Страница 65: ...thod reported values Result name Measurement Range Analysis wavelength correction Click row to select sample and update spectrum UV spectrum Right click graph area to view display options Measure samp...

Страница 66: ...method to be used for sample measurements with user defined settings Create new custom method From the NanoDrop Home screen select the Custom tab and select Custom Methods In the Manage Custom Methods...

Страница 67: ...and concentration of each standard and select the curve fit type Alternatively load a standard curve Enter or choose remaining custom settings as needed Choose Save Note Any errors in the method will...

Страница 68: ...measurement screen opens with the standard curve loaded You can begin measuring samples View or edit custom method Select Custom Method existing methods are listed in Select Method box along with thei...

Страница 69: ...orrection are used make sure your selected spectral range includes your specified baseline correction and or analysis correction wavelength For micro volume absorbance measurements and measurements ta...

Страница 70: ...ficient for 1 cm pathlength and use adjacent drop down list to select appropriate unit Equation below shows how extinction coefficient is used to calculate sample concentration c A b where c analyte c...

Страница 71: ...dictates that standards be measured from the lowest concentration of the standard analyte stock to the highest Concentration range of the standards must cover the dynamic range of the assay and the e...

Страница 72: ...olume measurements only When Automated Pathlength is selected software selects the optimal pathlength between 1 0 mm and 0 1 mm based on sample absorbance at the analysis wavelength For example when s...

Страница 73: ...orted in Data Table and Sample Details screens Unit Enter unit for reported result After a measurement the unit is reported in Data Table and Sample Details screens Edit Edit selected formula for curr...

Страница 74: ...n custom method From the Custom Methods screen select a custom method Select Follow the on screen instructions to measure a sample Export Custom Method Export a custom method in order to run it and st...

Страница 75: ...tings From Custom Methods screen select a custom method from the list of available methods From the drop down menu choose Edit Edit method settings as desired Choose Save Delete custom method From Cus...

Страница 76: ...6 Custom Applications Measure Custom 76 NanoDrop Eight User Guide Thermo Scientific...

Страница 77: ...ts Micro Volume Sampling How it Works 78 Set Up the Instrument 80 Measure a Micro Volume Sample 82 Sample Modes 86 Prepare Samples and Blanks 86 Basic Instrument Operations 92 Acclaro Sample Intellige...

Страница 78: ...surface tension to hold a small volume of sample between two pedestals upper and lower The patented sample retention system enables the measurement of highly concentrated samples without the need for...

Страница 79: ...nt is blanked a reference spectrum is taken of the blanking solution and stored in memory For each sample measurement the sample intensities along with the blank intensities are used to calculate the...

Страница 80: ...e set up to apply a baseline correction to each measurement to minimize any offset caused by light scattering particulates in the sample spectra The correction subtracts the absorbance value at a refe...

Страница 81: ...these specifications operating temperatures 5 C 35 C 41 F 95 F relative humidity non condensing 20 80 Locate the instrument away from air vents and exhaust fans to minimize evaporation After the inst...

Страница 82: ...angle for optimal use of the pipette guide Use calibrated precision pipettor 0 2 L volume range with well fitting low retention precision tips to apply sample material to instrument for measurement I...

Страница 83: ...Materials Recommended sample volumes To measure a micro volume sample Application Sample Volume Nucleic acid aqueous solution 1 La a Use 2 L for samples that contain materials that may reduce surface...

Страница 84: ...ement to complete Tip If Auto Blank is On blank measurement starts automatically after you lower the arm Lift the arm and clean all pedestals with a new laboratory wipe 4 Measure the first sample Pipe...

Страница 85: ...ring samples Select the End Experiment icon see previous image Enter an experiment name or leave the default experiment name Select Save Lift the arm and clean all pedestals with a new wipe If finishe...

Страница 86: ...e area This on screen sample plate will populate with Sample ID information from an imported sample ID file You can also manually enter Sample ID or other identifying information The sample status col...

Страница 87: ...before taking a measurement Avoid introducing bubbles when mixing and pipetting Choosing and Measuring a Blank The buffer used to resuspend a sample analyte can contribute absorbance Blanking minimiz...

Страница 88: ...ow concentration samples See below for details Good blanking buffer measured abs 0 04 Problems associated with blanking Residual sample was left on pedestal before blank measurement was performed Resu...

Страница 89: ...n rerun blanking cycle or measure new blank using new aliquot of appropriate buffer solution then measure new aliquot of unknown sample For most applications blank with the same buffer solution used t...

Страница 90: ...u plan to use for sample analyses Supplies needed lint free laboratory wipes calibrated precision pipettor 0 2 L buffer solution for evaluation To run a blanking cycle For quick demonstration watch mu...

Страница 91: ...lution onto the pedestal and lower the arm Start the sample measurement if Auto Measure is On lower arm if Auto Measure is off lower arm and select Measure Wait for measurement to complete The resulti...

Страница 92: ...op Eight Home Screen NanoDrop Eight Measurement Screens View History NanoDrop Eight General Operations NanoDrop Eight Home Screen These operations are available from the NanoDrop Eight Home screen His...

Страница 93: ...mation is described below Control options Serial number Instrument serial number Status Current status of the instrument Data storage Indicates location of database set where instrument is currently s...

Страница 94: ...and Intensity check should be run periodically according to the recommended maintenance schedule See Instrument Diagnostics on page 135 for information about how to run the available instrument diagn...

Страница 95: ...select sample and update spectrum select more rows to overlay spectra End experiment and export data Sample names click to edit UV absorbance spectrum for selected samples Measure blank solution Auto...

Страница 96: ...limit lines or enter values into the fields at the top of the spectrum Found peaks for the defined range are listed in the table below the spectrum Display options Right click graph to view Display mu...

Страница 97: ...icking the sample name field or by importing Sample ID file Edit sample base name After you measure a blank and before the first sample is measured click Sample Name field enter new base name press En...

Страница 98: ...the spectrum s vertical axis Micro volume absorbance measurements are normalized to a 10 0 mm pathlength equivalent Measurement Alerts The Acclaro Sample Intelligence technology built into the NanoDr...

Страница 99: ...ume Sample and Absorbance Detection Limits Auto Measure and Auto Blank Options Speed up sample analysis with the NanoDrop Eight Auto Measure and Auto Blank features which cause the instrument to start...

Страница 100: ...select End Experiment the End Experiment dialog box is displayed Click Save and you will have the option to Print or Export the experiment data Available options Note The End Experiment button is ena...

Страница 101: ...PC any USB port or to a network location Available export file formats comma separated values spreadsheet csv file tab separated values spreadsheet tsv file NanoDrop Eight software n8db file The file...

Страница 102: ...dot menu above the plate matrix user interface in the bottom left of the measurement acquisition screen 96 well plate allows users to keep track of sample measurements for samples stored in a standar...

Страница 103: ...ll either be filled bright green indicating no sample alerts or with an A in a circle indicating an Acclaro alert is present for that sample NanoDrop Eight tube rack accessory Sample channels Measurem...

Страница 104: ...ey wish to measure To activate inactive all channels at once click the circle in the column header area next to Sample Alternatively users can activate inactivate individual channels by clicking the c...

Страница 105: ...rement data from History Click the menu in the upper right of the measurement screen to access available menu options Home Return to NanoDrop Eight Home screen Import Import data from a USB flash driv...

Страница 106: ...ters include time range application type and any user defined labels see Manage Identifiers for information about adding and deleting labels Here is an example Export Selected Experiments Use Select i...

Страница 107: ...cess message select OK Delete Selected Experiments Select experiments to be deleted Delete selected experiments Filter experiments as needed use Search feature to find desired experiment Click the che...

Страница 108: ...r you open an experiment the software shows the UV or UV visible absorbance spectrum and associated data for the selected experiment much like it appears during a measurement The image below describes...

Страница 109: ...add one or more tags i e labels or metadata tags to an experiment to make the experiment easier to find Use the History to add labels to experiments assign existing labels view assigned labels and rem...

Страница 110: ...Manage Tags box enter a tag as an Identifier Click Save to add the tag Add additional tags as needed Click OK Remove a label from Home screen select History Click the menu of the experiment row and se...

Страница 111: ...automatically Export Selected Experiments You can export measurement data when you save the experiment or afterwards from the History Measurement data can be exported in three formats as comma separat...

Страница 112: ...for export each exported experiment has a corresponding file The filenames are the same as the experiment names The files are stored in a folder named NanodropEight followed by the instrument serial...

Страница 113: ...export path click Export Export data from History From Home screen open History Click the checkbox for the experiment s you want to export You can use the Search feature to find experiment Click Expo...

Страница 114: ...rom any experiment or all the measurements in the database Delete data from any measurement screen click a sample measurement row click Delete data from History from Home screen open History select ro...

Страница 115: ...elect Print In Print Review select the data you want to include in the data tables choose Print to confirm in the Print Preview window make sure the correct printer is selected and set other print opt...

Страница 116: ...rm in the Print Preview window make sure the correct printer is selected and set other print options as desired such as paper size and orientation Auto setting is recommended margin and alignment to a...

Страница 117: ...minant in dsDNA or dsDNA in RNA These models are specific to the source of the nucleic acid The mouse icon is representative of all mammalian sources of nucleic acid If you are measuring nucleic acid...

Страница 118: ...pear in the Sample Plate Map the data table and in History see below The icons are active in all three places the information remains with the data indefinitely even after it has been exported Contami...

Страница 119: ...e of guanidine HCl and guanidinium isothiocyanate detects species specific dsDNA contamination in the RNA application and detects species specific RNA contamination is in the dsDNA application for pro...

Страница 120: ...bance due to phenol 2 53 at the analysis wavelength 260 nm coefficient of variation for the measurement result uncertainty x 100 measurement result 4 22 a high CV indicates the measurement result is c...

Страница 121: ...titative approach to contaminant analysis Through sophisticated mathematical algorithms Acclaro analyzes the spectral data to identify probable contaminants in a sample and removes any contribution du...

Страница 122: ...haracteristics include absorbance ratios which indicate the presence of compounds that may interfere with sample measurements also referred to as purity ratios For more information watch the multimedi...

Страница 123: ...language requires a software restart Database The NanoDrop Eight uses a built in database Security Used when the optional Thermo Scientific SciVault software is installed The server location is autom...

Страница 124: ...tein editor Measurement Screen Display Options When performing measurements using the PC Control software right click the graph on the measurement screen to bring up the following display options Disp...

Страница 125: ...specified range You can enter the range by dragging the color coded limit lines or enter values into the fields at the top of the spectrum Found peaks for the defined range are listed in the table bel...

Страница 126: ...7 Learning Center Measurement Screen Display Options 126 NanoDrop Eight User Guide Thermo Scientific...

Страница 127: ...Thermo Scientific NanoDrop Eight User Guide 127 8 Maintenance Maintenance Schedule 128 Maintaining the Pedestals 129 Decontaminating the Instrument 133 Instrument Diagnostics 135...

Страница 128: ...ition pedestals Every 6 Months Recondition pedestals Run Intensity Check Run Performance Verification Run Pedestal Image Check If you are experiencing an issue with your system refer to the troublesho...

Страница 129: ...cid HF on the pedestals Fluoride ions will permanently damage the quartz fiber optic cables To prevent damage from spills keep containers of liquids away from the instrument Do not use a squirt or spr...

Страница 130: ...h a new laboratory wipe To clean the pedestals between users 1 Lift the arm and clean all pedestals with a new laboratory wipe 2 Pipette 3 5 L DI H2O onto the lower pedestal 3 Lower the arm and wait 2...

Страница 131: ...ower pedestal to flatten out preventing proper formation of the liquid column when the arm is lowered The resulting spectrum may look rough or jagged If samples flatten out on the pedestal rather than...

Страница 132: ...und to dry 3 Fold a clean laboratory wipe into quarters and use it to vigorously buff the surface of each pedestal Notice Support the instrument arm with one hand while you buff the upper pedestal to...

Страница 133: ...th one hand while you buff the upper pedestal to avoid damaging the arm 7 Fold a clean laboratory wipe into quarters and use it to vigorously buff the surface of each pedestal at least 50 times 8 Use...

Страница 134: ...l with a new laboratory wipe 2 Pipette 2 3 L diluted bleach solution see Supplies needed onto the lower pedestal 3 Lower the arm and wait 2 3 minutes 4 Lift the arm and clean all pedestals with a new...

Страница 135: ...iagnostics Thermo Scientific NanoDrop Eight User Guide 135 Instrument Diagnostics Every 6 months run the following performance and quality checks to verify instrument operation Intensity Check 136 Per...

Страница 136: ...cifications The test is automatically performed using the pedestal paths Supplies needed lint free laboratory wipes To run intensity check 1 From the Home screen select Intensity 2 Lift the instrument...

Страница 137: ...the intensity graphs of a single channel by clicking a single row from the Channel table or Shift Click to highlight multiple rows see image to the right A green check mark and banner will be displaye...

Страница 138: ...ansfer pipettes laboratory gloves Before you begin First make sure the pedestals are properly conditioned To test pedestal conditioning clean the upper and lower pedestal surfaces with a dry lint free...

Страница 139: ...gid structure to ensure the tips do not splay or skew when delivering the samples Tip It is suggested that this technique be practiced until consistent delivery to all eight positions is routine and q...

Страница 140: ...remove each aliquot and use a fresh aliquot of PV 1 for each measurement b In between each measurement remove PV 1 solution from all 16 pedestals upper and lower using a clean dry laboratory wipe 14...

Страница 141: ...ic NanoDrop Eight User Guide 141 3 Click End Experiment when done 4 To review results from a previous Performance Verification check select History from the Home screen and locate the Performance Veri...

Страница 142: ...8 Maintenance 142 NanoDrop Eight User Guide Thermo Scientific This page is intentionally blank...

Страница 143: ...ntific NanoDrop Eight User Guide 143 9 Safety and Operating Precautions Contents Operating Precautions 144 Safety Information 145 Note Be sure that all persons operating this system read the safety ma...

Страница 144: ...ous Materials Do not use hydrofluoric acid HF on the pedestals Fluoride ions will permanently damage the quartz fiber optic cables To prevent damage from spills keep containers of liquids away from th...

Страница 145: ...n the instrument itself The symbol indicates that there is additional safety information in the documentation and failure to heed the safety precautions could result in injury WARNING Indicates a haza...

Страница 146: ...trical shock in the vicinity Only qualified persons shall perform the related procedures Avoid fire hazard Do not test flammable or explosive samples Read and follow the associated instructions carefu...

Страница 147: ...injury If this equipment is used in a manner not specified in the accompanying documentation the protection provided by the equipment may be impaired CAUTION Avoid personal injury Perform only those p...

Страница 148: ...s damaged contact us Power Line Conditioning Accessories CAUTION Avoid shock hazard Only a qualified person using the appropriate measuring device shall check the line voltage current and frequency On...

Страница 149: ...able than the entire system including accessories typically uses Maximum power consumption and heat dissipation specifications for the spectrometer and accessories are shown below The values are appro...

Страница 150: ...ve to small volume measurements when using the pedestal for measurements on any of the NanoDrop instruments If you are measuring samples with high vapor pressures use an instrument with provision for...

Страница 151: ...m to HCl alcohol bleach acetone or other solvents as the adhesive securing the seal may be affected If the seal comes loose please contact us Biohazard or Radioactive Materials and Infectious Agents B...

Страница 152: ...e contaminated with biohazard or radioactive materials infectious agents or any other materials and or conditions that could constitute a health or injury hazard to employees Contact us if you have qu...

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