Thermo Scientific Dionex UltiMate 3000 Series, Руководство по эксплуатации

Страница: 176 / 270

UltiMate 3000 Series:
NCS-3500RS and NCP-3200RS
Page 168 Operating Instructions
Problem Probable Cause Remedial Action
Poor peak area precision The capillary connections are not
installed properly or they are
leaking.
Check and tighten the
capillary connections.
Consider using Viper
capillaries.
There are dead volumes in the
capillary connections.
Replace the conventional
(non-Viper) fitting
connections. Make sure that
the capillaries are connected
correctly. Consider using
Viper capillaries.
There are air bubbles in the pump
heads and/or flow meter.
Purge the pump ( → page 72).
After changing solvents, the
previous solvent has not been
flushed out completely.
Purge the pump ( → page 72).
The gradient is irreproducible. The pressure transducer offset
may not be correct. Check the
offset and calibrate the
pressure transducers if
required ( → page 172).
The sample is unstable and
decomposes.
Use new sample or change
the conditions. Cool the
sample in the autosampler if
possible.
Baseline fluctuations See the remedial actions
provided in the related
baseline sections further up in
this table.
The environmental conditions are
unstable.
Make sure that the
temperature and the humidity
are constant. Avoid draft.
Consider using the
thermostatted column
compartment.
Contamination occurs
somewhere in the system.
Flush the system with an
appropriate solvent.
For additional causes, see the
Autosampler manual.
→ Autosampler manual
If the problem is observed while
the module is used together with
a mass spectrometer, the nanoESI
emitter may be the cause.
→
Instrument manual