Appendix A
– Column Care
Thermo Scientific
Product Manual for CarboPac PA200 Columns
Page 35 of 37
065734-01
For Research Use Only. Not for use in diagnostic procedures.
A.
Disconnect column from the ED cell and direct the column effluent to waste. If your
system is configured with both a guard column and an analytical column, reverse the
order of the guard and analytical column in the eluent flow path. Double check that the
eluent flows in the direction designated on each of the column labels.
When cleaning an analytical column and a guard column in series, ensure that the guard
column is placed after the analytical column in the eluent flow path. Contaminants that
have accumulated on the guard column can be eluted onto the analytical column and
irreversibly damage it. If in doubt, clean each column separately.
B.
Wash the Dionex CarboPac PA200 with deionized water (18.2 megohm-cm) for about
30 minutes and then clean with 200 mM MSA for one to two hours at appropriate flow
rate.
C.
Wash the column with deionized water for about 30 minutes.
D.
Then clean the Dionex CarboPac PA200 with 100 mM KOH for at least one hour.
E.
Reconnect column to the cell and equilibrate the Dionex CarboPac PA200 to the desired
initial conditions and test the performance using QAR standard and eluent.
CAUTION
!
