5
– Troubleshooting Guide
Thermo Scientific
Product Manual for CarboPac PA200 Columns
Page 27 of 37
065734-01
For Research Use Only. Not for use in diagnostic procedures.
D.
A Dionex High-Pressure In-Line Filter positioned between the Pump and Eluent
Generator (or injection valve if and EGC is not installed) should be installed to prevent
particulates from blocking the system
5.1.2
Replacing Column Bed Support Assemblies for 1 mm column
If the column inlet bed support is determined to be the cause of the high back pressure, it should
be replaced. To change the inlet bed support assembly, refer to the following instructions, using
one of the two spare inlet bed support assemblies included in the Ship Kit.
A.
Disconnect the column from the system.
B.
Carefully unscrew the inlet (top) column fitting. Use two open-end wrenches.
C.
Remove the bed support. Turn the end fitting over and tap it against a benchtop or other
hard, flat surface to remove the bed support assembly. If the bed support must be pried
out of the end fitting, use a sharp pointed object such as a pair of tweezers, but be careful
that you do not scratch the walls of the end fitting. Discard the old bed support assembly.
D.
Place a new bed support assembly (provided with each 1-mm column) into the end
fitting. Make sure that the end of the column tube is clean and free of any particulate
matter so that it will properly seal against the bed support assembly. Drop the bed
support assembly into the end fitting, making sure that the bed support assembly is
centered at the bottom of the end fitting. Wrap the end fitting gently on a hard surface
to reorient the bed support assembly as necessary in order to properly situate the bed
support assembly in the end fitting.
If the column tube end is not clean when inserted into the end fitting, particulate matter
may obstruct a proper seal between the end of the column tube and the bed support
assembly. If this is the case, additional tightening may not seal the column but instead
damage the column tube or the end fitting.
E.
While holding the column in an inverted configuration, tighten the end fitting back onto
the column. Tighten it finger-tight, then an additional 1/4 turn (3.0 lbf-in). Tighten
further only if leaks are observed. It is important you do not overtighten the end fitting
or you will strip the threads on the column!
F.
Reconnect the column to the system and resume operation.
5.1.3
Filter Samples
Samples containing particulate material may clog the column inlet bed support. Filter samples
through a 0.45 µm filter prior to injection.
CAUTION
!
