3
– Operation
Thermo Scientific
Product Manual for CarboPac PA200 Columns
Page 19 of 37
065734-01
For Research Use Only. Not for use in diagnostic procedures.
3.5.2
System Re-Start Procedure for Dionex CarboPac PA200 0.4-mm Column
A.
.
B.
C.
Set the flow rate to 0.02 mL/min. Turn the pump and EGC power on. With Eluent Polarity set
to Basic Eluents, set KMSA
prog
to 50 mM and KOH_
prog
to 20 mM. Allow the system to run for
30 min.
D.
Turn the EGC power off.
E.
Keep the pump on at 0.02 mL/min for 10 min.
F.
Turn the pump off.
G.
Connect the OUTLET of the
to the ELUENT IN of the
Dionex RFIC capillary eluent degasser (Item # 22181-60202). Direct the ELUENT OUT of the
Dionex RFIC capillary eluent degasser to waste.
H.
Set the flow rate to 0.02 mL/min and turn the pump on for 10 min. Keep EGC power off.
I.
Turn the pump off.
J.
Connect the ELUENT OUT of the Dionex RFIC Capillary Eluent Degasser (Item # 22181-
60202) to 2 port of the injection valve.
K.
Install the CarboPac PA200 (0.4 x 50 mm) guard column and the CarboPac PA200 (0.4 x 250
mm) separator column to 3 port of the injection valve. Direct the OUTLET of the column to
waste.
L.
Set the flow rate to 0.01 mL/min With EGC power off, pump DI water through the system for
10 min.
M.
Turn the pump off.
N.
Connect the outlet of the separator column to the ED cell.
O.
Direct the outlet of the ED cell to waste.
P.
Turn the pump and EGC power on. Set to the required application condition.
Q.
When the pH reading of the ED detector shows pH > 11, turn the ED cell on.
R.
Allow the system to equilibrate for 30-45 minutes prior to collecting data.
