Thermo Scientific CarboPac PA200, Руководство по эксплуатации продукта

Страница: 22 / 37

4 – Applications
Thermo Scientific Product Manual for CarboPac PA200 Columns Page 22 of 37
065734-01
For Research Use Only. Not for use in diagnostic procedures.
4.2 Profiling of Inulin
Inulins are increasingly being used as functional food ingredients. Chain length distribution
profiles of commercial products such as those derived from inulin can be determined by using
HPAE-PAD with gradient elution. By adjusting the initial gradient profile, smaller oligofructose
chains can be distinguished from the inulin chains and separations up to and exceeding DP80 are
possible.
The CarboPac PA200, 3 mm column (Document No. 031992) is NOT compatible with the ICS-
6000 Dual EG mode but it is recommended to be used with manually-prepared sodium
acetate/sodium hydroxide eluents. The illustration of the application on the CarboPac PA200 3-
mm column is for comparison only, to demonstrate the comparable performance of the CarboPac
PA200, 1 mm and 0.4 mm columns with the ease of use.
Columns:
CarboPac PA200, 3-mm (guard + separator)
CarboPac PA200, 1-mm (guard + separator)
CarboPac PA200, 0.4-mm (guard + separator)
Gradient: CarboPac PA200, 3-mm:
0-45 min: 100-430 mM NaOAc in 100 mM NaOH
45-50 min: 430 mM NaOAc in 100 mM NaOH
50-65 min 100 mM NaOAc in 100 mM NaOH
CarboPac PA200, 1-mm:
0-45 min: 40 mM KMSA/60 mM KOH to 156 mM KMSA/22 mM KOH
45-50 min: 156 mM KMSA/22 mM KOH
50-65 min: 40 mM KMSA/ 60 mM KOH
CarboPac PA200, 0.4-mm
0-45 min: 40 mM KMSA/70 mM KOH to 190 mM KMSA/10 mM KOH
45-50 min: 190 mM KMSA/10 mM KOH
50-65 min: 40 mM KMSA/70 mM KOH
Flow Rate: CarboPac PA200, 3-mm: 0.5 mL/min
CarboPac PA200 1-mm: 0.063 mL/min
CarboPac PA200 0.4-mm: 0.010 mL/min
Detection: CarboPac PA200, 3-mm: PAD, Au on PTFE, 2 mil gasket, Ag/AgCl ref.
CarboPac PA200, 1-mm: PAD, Au on PTFE, 1 mil gasket, Ag/AgCl ref.
CarboPac PA200, 0.4-mm: PAD, Au on PTFE, 1 mil gasket, Ag/AgCl ref.
Samples: 5 mg/mL Inulin from chicory (Sigma)