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Techcomp FL970 Скачать руководство пользователя страница 23

18

Attention

Don’t pull the lamp by the positive wire when fixing the positive end with a nut. Be sure to

reserve a natural bending section of the positive wire, if the positive wire is pulled too tightly, the

xenon lamp will be at the risk of being damaged when lighting it.

1.6 Lamp Position Adjustment

In order to optimize the performance of the instrument, please adjust the position of the

lamp after the initial installation or replacement the lamp. The adjustment method is as follows

(Raman spectrum of distilled water is used for adjustment):

1) Turn on the instrument and the xenon lamp;

2)

After five minutes, turn on the computer and start the system initialization;

3)

Fill distilled water to a clean cuvette and then put it in the sample holder;

4)

Close the cover of the sample chamber

5)

Choose “Wavelength Scan” and set the following parameters: EM Slit and EX Slit are

10.0nm; Range of PMT value is 600

～

700V; Scan mode: EM scan; EM Start WL is 350nm,

EM End WL is 450nm; EX Fixed WL is 350nm; Speed is 240nm/min; Interval is 1.0nm;
Click “OK”.
Click “Start”, the instrument will start the wavelength scan.

Select the maximum absorption wavelength of Raman peak of distilled water after the scan.

Record the wavelength (it’s usually around 400nm).

Click “Time Scan” and set the parameters of time scan. The parameters are set as below:

EM WL is a, EX WL is 350nm, Resp. Time is 0.5S, Scan Time is 600S, click “OK”.

Click “Start”, and the instrument start the time scan.

Use a Phillips type screw driver to adjust the screw (Figure 1-9) on the focusing mirror

during the run process of “Time Scan”. Ensure the energy value of Raman peak to be

maximum (Figure 1-10).

Figure 1-9 Adjust the Phillips screws (Philips) of the focusing mirror

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Содержание FL970

Страница 1: ...0 INSTRUCTION MANUAL FL970 Fluorescence Spectrometer FL970 Fluorescence Spectrometer Edition 01...

Страница 2: ...Adjustment 13 1 4 1 Installation Site 13 1 4 2 Power 13 1 4 3 Precautions on Using Workstation 14 1 5 Xenon Lamp Installation and Adjustment 14 1 6 Lamp Position Adjustment 18 Chapter 2 Function 20 2...

Страница 3: ...ing and Print 50 3 8 3D Scan 50 3 8 1 Parameters Setting 51 3 8 2 Execute 55 3 8 3 Stop 56 3 9 Quantitation Measurement 57 3 9 1 Parameters Setting 57 3 9 2 Blank Measurement 61 3 9 2 Standard Sample...

Страница 4: ...manual is subject to change without notice for product improvement ABOUT THIS MANUAL This instruction manual is used to guide the user s operation of FL970 fluorescence spectrometer the operation met...

Страница 5: ...Affecting This Instrument During the operation of this instrument if there are other large equipment nearby the electromagnetic wave interference noise and vibration by such equipment will affect its...

Страница 6: ...d in the following cases even if they occur within the warranty period a Failure due to operation at a place not meeting the installation requirements specified by us b Failure due to power supply vol...

Страница 7: ...or written information or advice given by our dealers distributors agents or employees without our express permission shall not create a warranty or in any way increase the scope of this warranty Tech...

Страница 8: ...rd solutions and accuracy control samples shall be used stored and discarded as instructed by the respective suppliers 2 About this Instruction Manual a Information contained in this instruction manua...

Страница 9: ...he followings Dangger Indicates an imminently hazardous situation which if not avoided will result in death or serious injury There is no such sign in this instrument Warning Indicates a potentially h...

Страница 10: ...e rebuild replace the parts and use undesignated parts or use with the security protection equipment taken off by yourself because it s dangerous from a security standpoint When delivery please let ou...

Страница 11: ...and operating the instrument or dealing with data at the meantime can cause eye and physical fatigue if you continue to work in the same posture for extended periods When operating or dealing with dat...

Страница 12: ...d off When the power supply is restored normally the instrument can be restarted About fire Avoid smoking and using a fire in the vicinity of the instrument About warning signs on the instrument There...

Страница 13: ...ments that need to be used Do not use the programs and software from unknown sources to avoid computer virus infection In some cases antivirus software can be used to remove the virus but some viruses...

Страница 14: ...omputer please exit the application software of the instrument and then shut down the computer according to the correct steps in the operation system About sudden power failure of computer Sudden powe...

Страница 15: ...hed to FL970 fluorescence spectrometer 1 Dangerous voltage 500V 220V The warning label above is attached to the cover of the lamp source compartment and power supply socket 2 Attention to high tempera...

Страница 16: ...ment Limited deepens continuously the understanding of the corporate responsibility adheres to the road of the sustainable development of human society learns actively and abides by the relevant laws...

Страница 17: ...ply for this instrument 1 2 Unpacking Check After opening the packing please check the standard items against Table 1 1 Table 1 1 List of the host spare parts and accessories No Description Qty 1 Main...

Страница 18: ...g vibration or continuous weak vibration enough to be felt by the human body must not be transmitted to the instrument 4 Avoid strong magnetic field or strong electric field There should be no equipme...

Страница 19: ...s a on the outer cover of the lamp compartment and detach the outer cover from the instrument Figure 1 2 Then disconnect the socket to the fan Figure 1 3 2 Remove 3 screws on the inner cover of the la...

Страница 20: ...15 Figure 1 2 Remove the outer cover of the lamp compartment Figure 1 3 Pull out the connection line from the fan wire interface Figure 1 4 Remove the outer cover of the lamp compartment...

Страница 21: ...16 Figure 1 5 Schematic diagram of xenon lamp profile Figure 1 6 Light source safety switch bar on the inner cover of the light source...

Страница 22: ...17 Figure 1 7 Change the location of the light source safety switch Figure 1 8 Install the inner cover of the lamp compartment...

Страница 23: ...4 Close the cover of the sample chamber 5 Choose Wavelength Scan and set the following parameters EM Slit and EX Slit are 10 0nm Range of PMT value is 600 700V Scan mode EM scan EM Start WL is 350nm...

Страница 24: ...p and its representative is responsible for the installing and commissioning of FL970 fluorescence spectrometer Techcomp and its representative is to open the cover of FL970 fluorescence spectrometer...

Страница 25: ...e sample cell The monochromator is holographic type that consist of a concave diffraction grating and a slit components Part of the excitation light beam is split by a beam splitter is reflected by a...

Страница 26: ...ength repeatability Excitation wavelength 1nm Emission wavelength 1nm 6 Response time Raman peak of distilled water 0 98 about 2s 7 Signal to noise ratio S N ratio When the excitation and fluorescence...

Страница 27: ...rd curve 1 3 order Sample concentration Spectra arithmetic Save and open the spectra Spectra window processing Measure S N ratio automatically Measure D S ratio automatically 3D Scan Quantitation meas...

Страница 28: ...cable and turn on the power switch The computer will recognize a new hardware serial port If not it is necessary to install the driver the driver is available in the USB flash disk 3 3 Uninstall FLAn...

Страница 29: ...the instrument according to the following steps 1 Click the in the upper right corner of the software interface and the dialog box as shown in Figure 3 2 will appear Figure 3 2 Dialog box of the exit...

Страница 30: ...software Click the menu button of Function in the upper left corner and there are 4 drop down menus which are respectively File Instrument Self Diagnostics Settings File In the menu of File there are...

Страница 31: ...hown in Figure 3 5 Different spectra are displayed in different colors on the interface Select the data to display from the right side of the interface as needed Data 1 1 or Data 1 2 Using the mouse o...

Страница 32: ...the spectra to be hidden or seen as needed as shown in Figure 3 7 The data can be hidden by double clicking the color mark belts above the spectra with the mouse The color mark belt becomes gray and t...

Страница 33: ...3 8 Demo window of overlaying the spectra Overlaid spectrum is in the left of the dialog box Existing is in the right of the dialog box Move the left spectrum to the right as needed and click OK the s...

Страница 34: ...of overlaying the spectra Data without an asterisk marker can be removed from Existing to Overlaid Spectrum As shown in Figure 3 10 and Figure 3 11 Figure 3 10 Dialog box of removing spectra from Exis...

Страница 35: ...tting options Click them and set the correspongding wavelength as needed As shown in Figure 3 12 Figure 3 12 Dialog box of setting wavelength 2 Concentration Measurement Click Concentration Measuremen...

Страница 36: ...Drift Debug internal control indicators Change of Fluorescence Value Debug internal control indicators S N RMS and S N RMS BG The PMT value can be set in the setting of Wavelength Scan or Time Scan a...

Страница 37: ...32 Figure3 14 Demo window of Settings Close all data views Click the icon as shown in Fig 3 15 all displayed spectra and data can be deleted Figure3 15 Icon of closing all the data...

Страница 38: ...e 3 16 Figure 3 16 Function interface of wavelength scan 3 6 1 Parameters Setting Click the Setting button in the right toolbar of WL Scan for parameter input the parameter setting window consists of...

Страница 39: ...Instrument As shown in Figure 3 18 the introduction of various system parameters of the instrument is as follows Figure 3 18 Dialog box of Instrument parameters for WL Scan Slit EX Slit and EM Slit o...

Страница 40: ...box of wavelength scan parameters for WL Scan Scan Mode There are three Scan Modes EM Scan EX Scan and Synchronized Scan from top to bottom Start WL Start value of wavelength scan can be input from 20...

Страница 41: ...k valley can be judged Recommend value is 5 Sensitivity The number required of the data points between peak and valley which the peak valley can be judged Recommend value is 2 Figure 3 20 Dialog box o...

Страница 42: ...of WL Scan and the status bar of the instrument prompts the conversion from Ready to Scanning as shown in Figure 3 22 Note the Start Stop and Setting buttons in the toolbar are valid the rest are inva...

Страница 43: ...f the data file at the end of the measurement 2 Smooth and Derivative Click the corresponding buttons as shown in Figure 3 23 the spectrum and data will become the spectrum and data that have been smo...

Страница 44: ...ea Click Calculate spectrum area button the window as shown in Figure 3 25 appears Enter start wavelength and end wavelength and click Calc Area to get the result S1 represents the area of the spectru...

Страница 45: ...n in Figure 3 26 appears There are four pages in the window of Property of WL Scan For the first three pages leave them as the default The general information is displayed in the first page The sample...

Страница 46: ...ters button for WL Scan The second page shows the system parameters of the instrument and the main parameters of wavelength scan as shown in Figure 3 27 Figure 3 27 Dialog box of the system parameters...

Страница 47: ...cessing after clicking the View parameters button for WL Scan The fourth page is Print Export as shown in Figure 3 29 Print report export file the checked items are displayed and the font and size can...

Страница 48: ...main interface of the software to save the current spectrum and data Click the button to exit the interface of wavelength scan 8 Check in front of Data in Fig 3 29 and then click the OK button Click...

Страница 49: ...44 Figure 3 30 Dialog box of Save As after clicking the excel icon for WL Scan Figure 3 31 Excel display window after clicking the Save button...

Страница 50: ...ure 3 32 Function interface of Time Scan 3 7 1 Parameters Setting Click Setting button in the toolbar on the right of time scan interface to input the parameters The window of Setting consists of 5 pa...

Страница 51: ...og box of General parameters for Time Scan 2 Instrument As shown in Figure 3 34 the introduction of various system parameters of the instrument is as follows Figure 3 34 Dialog box of Instrument param...

Страница 52: ...y to reset in the interface of Setting 3 Time Scan As shown in Figure 3 35 the parameters required for wavelength scan are described as follows Figure 3 35 Dialog box of time scan parameters for Time...

Страница 53: ...is 2 Sensitivity The number required of the data points between peak and valley which the peak valley can be judged Recommend value is 5 Figure 3 36 Dialog box of Data Processing parameters for Time S...

Страница 54: ...8 Note the Start Stop and Setting buttons in the toolbar are valid the rest are invalid Figure 3 38 Display window of the status of clicking the Start button of Time Scan 1 The data window appears aft...

Страница 55: ...9 Display window of the end of time scan 3 7 3 Data Processing and Print As 3 6 3 3 8 3D Scan initialization completion and the status of the instrument will show as Ready click 3D Scan in the left of...

Страница 56: ...eral Instrument 3D Scan Data Processing Plot 3D 1 General As shown in Figure 3 41 the company name and the operator can be entered and the instrument information can also be displayed Figure 3 41 Dial...

Страница 57: ...MT Value PMT Mode refers to the boosting mode of the photomultiplier PMT Value can be entered in manual mode the range is 0 900V Open the cover plate of the sample room the high voltage will automatic...

Страница 58: ...be less than the end wavelength at least 1nm EX End WL End EX wavelength of 3D scan can be input from 200nm to 900nm with a minimum step size of 0 2nm the end wavelength must be more than the start w...

Страница 59: ...45 the parameters that are necessary for plotting are described as follows Z Axis The input range is 70000 70000 The recommended range is 0 70000 EX wavelength and EM wavelength show the maximum and m...

Страница 60: ...re 3 47 Icons of on the upper of the interface A Reset B Spectra arithmetic C Wireframe Contour Plane Contour line Surface change the display mode D On off color display the color spectra or black and...

Страница 61: ...ing any location of 3D spectra Figure 3 48 are the cross section diagrams of EX and EM the EX cross section and EM cross section of the current click location can be selected when clicking the 3D spec...

Страница 62: ...Figure 3 49 Figure 3 49 Function interface of Quantitation 3 9 1 Parameters Setting Click Setting button in the toolbar on the right of Quantitation to input the parameters The window of Setting cons...

Страница 63: ...Instrument As shown in Figure 3 51 the introduction of various system parameters of the instrument is as follows Figure 3 51 Dialog box of Instrument parameters for Quantitation Slit EX Slit and EM S...

Страница 64: ...in the interface of Setting 3 Quantitation Figure 3 52 Dialog box of parameters for Quantitation EX WL and EM WL can be selected from 200nm to 900nm Calibration Standard sample fitting Manual Fitting...

Страница 65: ...parameters for Quantitation The standard sample name concentration comment can be entered and sequence number will be numbered automatically The information of the standard samples can be inserted app...

Страница 66: ...tting are described as follows Max and Min of X Axis Max and Min of X Axis can be determined according to the values of start wavelength and end wavelength which can t be changed Max and Min of Y Axis...

Страница 67: ...med automatically the Quantitation measurement of Blank logo will be red as shown in Figure 3 57 standard sample measurement and sample measurement will automatically deduct the blank data after being...

Страница 68: ...only for understanding the operation no specific data value Figure 3 59 Display window of the end of Std Meas for Quantitation After the completion of Std Meas the measurement of unknown sample can b...

Страница 69: ...64 Figure 3 60 Display window of the end of Smpl Meas for Quantitation 3 9 4 Measurement stop Click End to end the measurement...

Страница 70: ...rong acid or alkali which should be removed from the instrument by removing the fixed screws and washed with water and dried At the end of the detection the organic solution should be removed from the...

Страница 71: ...62 16 building No 201 Mingyi RD Songjiang District Shanghai 86 21 67687200 86 21 67687190 sh factory techcomp cn www techcomp cn Shanghai Techcomp Instrument LTD...

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