4. Operating Instructions
34
HYDROFLEX PLUS No. 30199906 Rev. No. 1.1
2022-01
4.4
Wash Modes
The instrument can wash a microplate using the following wash modes:
Plate Mode
Each program step is performed on all of the selected strips of a microplate
sequentially, before proceeding to the next step.
The entire plate or the defined plate range is processed for the defined soaking time.
Strip Mode
The entire wash program is performed on one strip or two consecutive strips of a
microplate before proceeding to the next strip(s) (1 strip for 8-way manifold or two
strips for 16-way manifold). The strip or group of two strips is processed for the
defined soaking time, before proceeding to the next strip or group of strips.
4.5
Washing Positions
Wash, Dispense, and Aspirate steps can be defined in a wash program with the
following Z-positions:
Overflow
For ELISA assays and Cellular assays. Overflow washing consists of a simultaneous
aspiration and dispense step. It creates a circular flow of wash buffer in the well and
ensures that the topmost parts of the well are also washed.
Bottom
For ELISA assays.
Custom
Any necessary washing position can be selected; the custom positions are not saved
as *.pdf files in comparison to the overflow and bottom position.
Move Overflow
If Move Overflow (MOVE OVER.) is selected, the manifold moves stepwise from the
bottom position to the Overflow position during dispensing. It is recommended for cell
wash applications or any applications which must be treated with care.
Move Custom
If Move Custom (MOVE CUST.) is selected, the manifold moves stepwise from the
bottom position to the user-defined Custom Z- position during dispensing. It is
recommended for cell wash applications or any applications which must be treated
with care.
Z-Position Bottom
Z-Position Custom
Z-Position Overflow
Figure 4.2 Wash Position Diagrams
MOVE OVER.
Overflow Position
Movement of
manifold
MOVE CUST.
Custom Position
Movement of
manifold
Bottom Position
Bottom Position
Figure 4.3 Movement Diagrams for Z-positions with MOVE
