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LISST-
HOLO User’s Guide
.TIFF files output by HOLO_Batch
If you selected to output dep/mon images, two .TIFF files will be created
for every hologram process. They will be placed in the reconstructed
images folder
The
–mon suffix image is a montage of all the in focus particles that
were found in all the planes within an ROI.
The scale bar length can be adjusted prior to processing in the
Advanced section of HOLO_Batch.
Note that only the part of the hologram covered by the ROI is displayed
on the
–mon image. The rest of the hologram was not analyzed and
therefore cannot be displayed.
The
–dep suffix image is a color coded image, where the particles within
the ROI have been colored according to their depth of focus.
As with the
–mon image, only the part of the hologram covered by the
ROI is displayed on the
–dep image.
The scale bar is all blue and displayed in the bottom left corner. The
color bar in the bottom right corner shows the location of the particles
relative to the transmit window. Red particles are very close to the
transmit window, blue particles very close to the receive window.
Using a pair of 3-D glasses will display the particles in a 3-D effect,
where the red particles will appear closer to the viewer than the blue
particles.
.CSV files output by HOLO_Batch
A composite file with the name
firsthologram
_
lasthologram
_All.csv, where firsthologram and lasthologram are the filenames of
the first and last holograms that were selected for processing. This file is always generated for each run of Holo Batch. This
composite file has all the information about sample date and time, water depth, temperature, and the size distribution (given as
the volume concentration in (µl / l) per size bin).
Finally, the composite file has in its first row the lower limit of the size bin for the size distributions in columns 26:75. In the 2
nd
row is the mid-point of the size bin for the size distributions in columns 26:75 and in the 3
rd
row is the upper limit of the size bin
for the size distributions. The table below shows the parameters in columns 1-75 of the .CSV file.
Column #
Value
Unit
1
Year (YYYY)
N/A
2
Month (MM)
N/A
3
Day (DD)
N/A
4
Hour (HH)
N/A
5
Minute (MM)
N/A
6
Second (SS)
N/A
7
1/100s of a second (hhh)
NOTE: Currently not available. NaN (Not a Number) displayed instead.
8
Depth
m
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