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Schambeck RI 2000, Руководство по эксплуатации

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Schambeck SFD GmbH 

 

Refractive Index Detector RI2000 

 

 

SFD101.01.3x.EN / 2014-07-10 

19 

http://www.schambeck-sfd.com 

 

12 

P

RINCIPLE OF 

D

ETECTION

 

The RI2000 refractive index detector acts as a differential refractometer which measures 

the deflection of a light beam as a result of different refractive indices of solutions in the 

reference and the sample chamber of the flow cell. 

The beam of a tungsten lamp passes a convex lens and two slip apertures (slit aperture 1 

and slit aperture 2) before reaching a second convex lens. After this the light passes the 

flow cell which is diagonally separated into two chambers (sample and reference chamber). 

Behind the flow cell a mirror is placed which  reflects the beam through the flow cell,  the 

second convex lens and the second slit aperture (slit aperture 2) toward a light sensor. 

The light sensor is placed under the light source and consists of two photo diodes. The both 

diodes generate a current which is proportional to the light 

intensity reaching the sensor’s 

surface. 

 

 

12.1 T

HE 

O

PTICAL 

S

YSTEM OF THE 

RI2000 

 

 

 

 

Содержание RI 2000

Страница 1: ...RI 2000...

Страница 2: ...Version 1 1 Printed 09 07 2014 File RI2000 V4 0 SFD English FINAL 07 2014 doc Last modified Makowski...

Страница 3: ...7 THE MENU BUTTON 13 9 8 THE ENTER BUTTON 13 9 9 THE IN PORT 13 9 10 THE OUT PORT 13 10 THE BACK SIDE OF THE RI2000 14 10 1 OUTPUT CONNECTORS 14 10 1 1 THE INTEGRATOR SIGNAL OUTPUT 14 10 1 2 THE RECOR...

Страница 4: ...AL ANALOG CONVERTERS 40 15 4 CALIBRATING THE RI2000 41 15 4 1 HOW TO CALIBRATE 42 15 5 SERVMODE OVERVIEW 45 15 6 SERIAL COMMUNICATION 46 16 MAINTENANCE OF THE RI2000 47 16 1 IDENTIFYING HARD AND SOFTW...

Страница 5: ...ACT ADDRESS In case of problems with your RI2000 refractive index detector please contact your local distributor or Schambeck SFD GmbH Schambeck SFD GmbH Rh ndorfer Strasse 51 D 53604 Bad Honnef Germa...

Страница 6: ...ex detector In case of any question please do not hesitate to contact you local distributor or Schambeck SFD GmbH directly 3 ELECTRICAL WARNINGS Before opening the housing of the instrument make sure...

Страница 7: ...h appropriate cleaning agents only Make sure that no liquid gets inside the detector Liquid inside the housing may cause electrical short circuits which may result in the damage of the instrument To p...

Страница 8: ...tector After use of salt containing solvents such as buffers the detector should be purged with distilled water Make sure that the 6 bar 0 6 MPa pressure limit of the flow cell is not exceeded Make su...

Страница 9: ...g signal output Recorder integrator Noise analog signal 10 nRIU 10 V Noise digital output 5 nRIU 5 V Drift 1 RIU h 1mV h Integrator output 1 2 V fix Recorder output 1 2 V adjustable Recorder range 8 s...

Страница 10: ...EN 2014 07 10 8 http www schambeck sfd com 6 PHYSICAL SPECIFICATIONS Dimensions 220 mm 165 mm 350 mm W H D Weight 12 kg Voltages Mains adaptor with voltage selector 100 120 V 220 240 V Frequency 50 or...

Страница 11: ...rantee Repair works covered by the guarantee may only be performed by Schambeck SFD GmbH or licensed distributors The following cases are not covered by the guarantee Improper use e g capacity overloa...

Страница 12: ...nless steel capillary 0 25 mm inner diameter 1 59 mm outer diameter for sample entry 1 EA PTFE tube 0 80 mm inner diameter 1 59mm outer diameter for sample exit 2 EA Screw connection for sample entry...

Страница 13: ...ay contains four lines with 20 characters each Line 1 shows the current temperature of the optical bench in C Line 2 shows the optical balance in Line 3 shows the current detector signal in mV The val...

Страница 14: ...olarity of the detector signal will be changed A negative signal like e g 5mV will be transformed into a positive signal 5 mV when the polarity mode is activated A red LED will light up when this mode...

Страница 15: ...rformed on a parameter 9 9 THE IN PORT This is the entrance for the mobile phase coming from the column Connect the capillary coming from the column to this port using screw connectors 9 10THE OUT POR...

Страница 16: ...onnect the integrator using a shielded signal cable to the integrator output Int Out Make sure that the polarity is correct 10 1 2 THE RECORDER SIGNAL OUTPUT To record data using an analog recorder co...

Страница 17: ...signal to the instrument 10 2 3 THE EXTERNAL PURGE SIGNAL Using this connector Purge you can use an external signal to activate the detector s purge mode NOTE All TTL signals handled by the RI2000 det...

Страница 18: ...a leakage inside the housing mobile phase can leave the housing using the tube You might connect an additional waste tube to this exit 10 7MAINS ADAPTOR Use the power cable which was delivered with t...

Страница 19: ...mixture to perform your analytical separation it is recommended to mix the different components manually before using the solvent The use of a gradient pump to generate a solvent mixture is not possib...

Страница 20: ...h sensitivity it is recommended to use the recorder output Rec Out as you can use the instrument s internal signal amplifier which might amplify the detector signal up to 16 times 11 3CONNECTION TO A...

Страница 21: ...nd two slip apertures slit aperture 1 and slit aperture 2 before reaching a second convex lens After this the light passes the flow cell which is diagonally separated into two chambers sample and refe...

Страница 22: ...urement is performed at constant temperatures as the refractive index is highly dependent on the temperature The temperature for the optical bench can be selected in the range from 35 C to 55 C After...

Страница 23: ...t exchanger going towards the flow cell Now the stream enters the sample chamber of the flow cell Leaving the flow cell passing the heat exchanger again the liquid reaches the T connector and after th...

Страница 24: ...wing figure illustrates the liquid stream in purge mode with ASCO valve For the new ASCO valve the RI2000 Firmware 4 2 is essential The purge mode should be activated until a stable baseline is reache...

Страница 25: ...Schambeck SFD GmbH Refractive Index Detector RI2000 SFD101 01 3x EN 2014 07 10 23 http www schambeck sfd com 14 OPERATING THE RI2000 DETECTOR 14 1OVERVIEW FIRMWARE VERSION 3 2C...

Страница 26: ...shown in the display the LEDs will light up for a short time and an acoustical signal will occur The initialization procedure will be finished after approx six seconds and the detector will switch au...

Страница 27: ...ch in C Line 2 shows the optical balance between the two photo diodes of the light sensor in which corresponds with the relative position of the light beam on the surface of the light sensor Line 3 sh...

Страница 28: ...tings ComRS232 Settings for serial RS232 port Another function of the status line is the input of new parameter settings by the user The symbol represents the four ARROW keys stands for the ENTER butt...

Страница 29: ...up when operated As a result of this the temperature can be controlled in a range from 35 C up to 55 C only To change the temperature setpoint press the ArrowUp to switch to higher temperatures or use...

Страница 30: ...64 C In this case the error message will be shown in the display and a periodic acoustic signal occurs The acoustic signal will stop when the temperature reaches the range between 9 C and 64 C NOTE Sh...

Страница 31: ...fferent modes for signal smoothing RAW No signal smoothing FAST Signal smoothing by calculating the average in a time interval of 0 4 seconds MEDIUM Signal smoothing by calculating the average in a ti...

Страница 32: ...s to amplify or attenuate the detector signal at the recorder output RecOut If the amplification mode is set to 1 1 the signal at the recorder output Rec Out corredsponds to the signal at the integrat...

Страница 33: ...us line is shown in the display By pressing the buttons ArrowUp and ArrowDown you can either add a static voltage to each data point recorded by the detector detector offset or define a maximum voltag...

Страница 34: ...thout accepting press the ENTER button 14 7 2 RECORDER EXTENT To adjust the recorder extent press the ArrowUp button until the following status line is displayed Using the ArrowUp button you can switc...

Страница 35: ...output Is the parameter UseMarker set to NONE no marker signal can be sent to the recorder Pressing one of the buttons ArrowUp or ArrowDown changes the setting to ARROW To accept this setting press t...

Страница 36: ...ou may adjust the parameters for the serial data interface If the mode is set to LOCK no detector is sent to the RS232 port Use this setting only in that case you want to record your data using an ana...

Страница 37: ...ter Enter Enter N o r m M o d e In this mode the user can adjust several parameters fort he operation of the RI2000 refractiv index detector The Norm Mode is used to Display temperature C optical bala...

Страница 38: ...The display will change from normal measuring mode NormMode to service mode ServMode The numerical values for temperature optical balance and the current detector signal are now displayed with three d...

Страница 39: ...the firmware version number or the instrument s serial number press the MENU button in service mode until the menu SwHwInfo is shown in the status line Then press the ENTER button The display will sh...

Страница 40: ...s mode press the MENU button until the sub menu ViewFine is shown in the status line Now press the ENTER button and the display will change to the following information The values in line two represen...

Страница 41: ...art of the light sensor Smpl Sample voltage Corresponding to the light intensity on the sample side of the light sensor Rfrn Reference voltage Corresponding to the light intensity on the reference sid...

Страница 42: ...ion allows the user to apply a defined signal to the recorder output and the integrator output NOTE Make sure that the signal amplification for the recorder output is set to 1 1 before using this func...

Страница 43: ...of the optical system only Under normal operation conditions a re calibration of the RI2000 is not needed To calibrate the signal response of the instrument you need a test standard solution of exactl...

Страница 44: ...ll be shown in the display DifW Difference with water SumW Summ with water Dift Difference with test solution SumW Summ with test solution By pressing the ENTER button you can see the current SPAN To...

Страница 45: ...n The display will change to the following view The signal is recorded and checked In the display line two the difference voltage Diff 0100 in this example and the sum voltage Summ 5030 in this exampl...

Страница 46: ...ibration process is confirmed by a displayed message Press the ENTER button to finish the calibration process The new calibration factor is shown in the upper right corner of the display The four meas...

Страница 47: ...Schambeck SFD GmbH Refractive Index Detector RI2000 SFD101 01 3x EN 2014 07 10 45 http www schambeck sfd com 15 5 SERVMODE OVERVIEW...

Страница 48: ...LOW 0 4 Hz serial port open 0 4 data point sec sent 1 Hz serial port open 1 data point second sent 2 Hz serial port open 2 data point second sent 5 Hz serial port open 5 data point second sent 10 Hz s...

Страница 49: ...ument s serial number you can get this information in the service mode of the detector Press the buttons ArrowLeft and ArrowRight at the same time for approx three seconds The display will switch into...

Страница 50: ...appear directly after switching on the instrument 16 2 1 MALFUNCTION OF THE TEMPERATURE SENSOR There are two possible error messages indicating problems with the temperature sensor A more detailed de...

Страница 51: ...message noAD shows up the analog digital converter did not connect to the micro controller Switch of the instrument wait for ten seconds and try to start again If the problem remains contact the tech...

Страница 52: ...ctive if there is a detector signal Is the message intensity OR SIGNAL too low displayed the voltage USumm is below 1 7 V The reason is low light intensity reaching the light sensor To resolve this pr...

Страница 53: ...ollowing table xx 1H sample voltage beyond 5 V xx 2H reference voltage beyond 5 V xx 1L sample voltage below 0 V xx 2L reference voltage below 0 V The sample voltage corresponds to the light intensity...

Страница 54: ...switched on If the error message RC is shown in the display the controller was not able to load the internal configuration during the initialization In this case for example the calibration factor is...

Страница 55: ...cedure you can follow the changes by observing the change of the detector signal or more detailed by observing the difference and sum voltage For the calculation of the different voltages the detector...

Страница 56: ...eck sfd com NOTE There are two adjustment screws for the mirror Keep in mind that you might have to turn the other screw Anyway you should turn the screws not more that or turns Adjust the screws unti...

Страница 57: ...he following steps Switch off the instrument Unplug the mains cable to prevent electrical shock when opening the housing NOTE Some operations during the adjustment have to be done when the instrument...

Страница 58: ...EN 2014 07 10 56 http www schambeck sfd com Loosen the holding screw of the light source and remove the lamp Loosen the screw connector of the lamp s power supply at the pre amplifier board labelled L...

Страница 59: ...erial number of you instrument is 0312xxx the points are labelled Lampe and GND if the serial number of your instrument is 0401xxx the points are labelled Lampe ULmp and GND To find your serial number...

Страница 60: ...com Make sure that only 2 3 of the slit are illuminated by the light source This is illustrated in the following figure NOTE The projected picture at the light sensor should be a sharp one The full h...

Страница 61: ...ical balance should be 0 20 0 To ensure a stable temperature the instrument should be switched on for several hours before performing this test Press the PURGE button Make sure the purge mode is activ...

Страница 62: ...he difference voltage in mV and corresponds to the difference of intensity reaching the sample side and the reference side of the light sensor Summ represents the sum voltage in mV and corresponds to...

Страница 63: ...8 and 69 you can find a schematic view of the main circuit board with the marked positions of the test points If the sum voltage is only a few millivolt the light bulb does not work Check the power ca...

Страница 64: ...d before opening the housing of the instrument Remove the stainless steel capillaries from the valve body NOTE Remind the position of the capillaries for proper re connection Loosen the control cable...

Страница 65: ...e flow cell Drop of the sum voltage below 4500 mV Make sure that your sample chambers are washed and filled with distilled water without bubbles before checking the sum voltage Noisy baseline may be c...

Страница 66: ...son it is not recommended to store the detector for a longer time with aqueous solvents inside For long time storage it is recommended to purge the flow cell with ethanol followed by air If possible w...

Страница 67: ...radiated of electronic compounds In the interval from 35 C up to 55 C the temperature can be selected in 1 C steps The temperature difference of 6 C has to be considered If the heating is activated a...

Страница 68: ...e chapter 15 1 to locate the test points on the board The figure on page 70 shows the board used in instruments if the 0312xxx series The figure on page 71 shows the board used in instruments of the 0...

Страница 69: ...RI2000 Nessesary for all typs of flow cells RI2000 001 Flow cell analytical version RI2000 RI2000 003 Flow cell holder analytical version RI2000 RI2000 030 Flow cell micro version RI2000 RI2000 005 F...

Страница 70: ...Schambeck SFD GmbH Refractive Index Detector RI2000 SFD101 01 3x EN 2014 07 10 68 http www schambeck sfd com 19 TEST POINTS FOR LAMP VOLTAGE FOR SERIAL NUMBER 0312XXX...

Страница 71: ...Schambeck SFD GmbH Refractive Index Detector RI2000 SFD101 01 3x EN 2014 07 10 69 http www schambeck sfd com 20 TEST POINTS FOR LAMP VOLTAGE FOR SERIAL NUMBER 0401XXX...

Страница 72: ...Schambeck SFD GmbH Refractive Index Detector RI2000 SFD101 01 3x EN 2014 07 10 70 http www schambeck sfd com 21 VOLTAGE TEST POINTS OVERVIEW FOR 0312XXX SERIES...

Страница 73: ...Schambeck SFD GmbH Refractive Index Detector RI2000 SFD101 01 3x EN 2014 07 10 71 http www schambeck sfd com 22 VOLTAGE TEST POINTS OVERVIEW FOR 0401XXX SERIES...

Страница 74: ...Laborger te 89 336 EWG Elektromagnetische Vertr glichkeit EMV DIN EN 50081 Fachgrundnorm St raussendung EN 55022 Messung der Funkst rspannung von 150 kHz bis 30 MHz EN 55022 Messung der Funkst rfeldst...

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