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Olympus SLIDEVIEW VS200, Инструкция по эксплуатации

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18  VS200 device configuration

shading correction. See

Shading correction for fluorescence observation

methods auf Seite 160

.

18.7 VS200 LED lamp voltages

18.7.1 Orca Flash 4.0 voltages (%)

Observation method

Objective

BFMono

(AS 75%)

DFMono

(AS max)

PHMono

(AS 75%)

2x PLN

0.6

not defined

not defined

4x UPLFLN

0.6

-

not defined

10x UPlanXApo

0.8

-

-

20x UPlanXApo

0.5

not defined

-

40x UPlanXApo

0.7

not defined

-

40xUplanXApoS

60x UPlanXApoO

-

not defined

-

100x UPlanXApoO

-

not defined

-

18.7.2 Orca Fusion voltages (%)

Observation method

Objective

BFMono

(AS 75%)

DFMono

(AS max)

PHMono (AS 75%)

2x PLN

1.8

not defined

not defined

4x UPLFLN

1.2

100

not defined

10x UPlanXApo

1.8

100

60

20x UPlanXApo

2.1

not defined

100

40x UPlanXApo

5.7

not defined

100

40xUplanXApoS

not defined

100

60x UPlanXApoO

6.8

not defined

100

100x UPlanXApoO

2.3

not defined

100

- 97 -

Instructions for use

OLYMPUS VS200 System

Содержание SLIDEVIEW VS200

Страница 1: ...Instructions for use SLIDEVIEW VS200 Optical Microscope and Accessory...

Страница 2: ...s without obligation to notify the purchaser In no event shall Olympus Soft Imaging Solutions GmbH be liable for any indirect special incidental or consequential damages arising out of purchase or use...

Страница 3: ...16 5 Scope of supply 17 6 System diagram 20 6 1 Available fluorescence components 20 7 Trays 22 7 1 Tray description 22 7 2 VS200 Tray types 22 8 Specifications 24 9 Unboxing 26 9 1 VS200 Scanner 26 9...

Страница 4: ...Add or replace filter U FFWO 51 11 2 5 TV 1 0x adapter 52 11 3 Monochrome camera 52 11 3 1 Orientation of the monochrome camera 53 11 4 X Cite adapter 56 11 5 4x Objective 57 11 6 LED light source 57...

Страница 5: ...3 VS 304M voltages 98 18 7 4 VS 264C voltages 98 19 How to insert a slide into a tray 99 19 1 How to insert a tray into the VS200 scanner 99 19 2 Insert a tray into the VS200 loader 100 20 Calibrate V...

Страница 6: ...8 23 1 OlyVIA 178 23 2 VS200 ASW Desktop 178 23 3 NetImage Server SQL NIS SQL and Webinterface 178 24 Troubleshooting 179 24 1 Hardware not available 179 24 2 No camera error 179 24 3 Tray not active...

Страница 7: ...configuring a liquid dispenser and upgrading to a VS200 loader are not in the scope of this document These must only be installed and adjusted by technical service of Olympus 1 1 Notes on qualificati...

Страница 8: ...anuals for the components of the system Take special note of the safety instructions they contain 1 4 Symbols in this documentation Tools required for performing the steps Preconditions for the subseq...

Страница 9: ...o the product can occur if ignored Useful tips and important information This symbol indicates useful notes tips and important information 2 2 Caution labels Indicates a non specific general hazard Th...

Страница 10: ...n 1 Abnormal state of an image 2 Abnormal state of file property such as a file name and file date and time 3 Disappearance of an image Confirm edit or save the image for backup through care of the ob...

Страница 11: ...id hardware states when forcing application to shut down The application has no means of resisting a forced shutdown e g due to a forced Windows Update or application kill via the Task Manager power l...

Страница 12: ...work environment note the following points Make sure that the network has appropriate virus protection The installation of operating system service packs security patches or third party software updat...

Страница 13: ...is The X Cite Turbo light source is not available in all countries Follow the safety precautions at all times during operation and maintenance of this product Non observance may result in eye injury o...

Страница 14: ...representative has to be contacted first 3 1 Betriebs und Lagerbedingungen Positioning the system Only use the system indoors and in a laboratory or laboratory like environment Elevation 2000m maximum...

Страница 15: ...e conditions The maximum permissible range for the ambient temperature for storage of the VS200 system is 25 to 55 C The maximum permissible relative humidity range for storage is 10 to 95 The maximum...

Страница 16: ...4 Tools and accessories 2 5 mm 3 mm with ball end 4 mm 5 mm hex keys 16 Instructions for use 4 Tools and accessories OLYMPUS VS200 System...

Страница 17: ...ront Housing panel top back Housing beam splitter cover 4 handles to carry the system PLN2x UPLANXAPO20x 1 1x3 inch tray VS200 standard computer with mouse and keyboard HP TFT 27 inch Instruction manu...

Страница 18: ...filter 1x Glas filter U FFWO T3 U FFWR X Cite Xylis Fluorescence kit C The Fluorescence kit C is not available in all countries Box IX3 RFACA IX3 RFALFE UPFLN4x U FF filter cube 2x Black out filter 1...

Страница 19: ...5 Scope of supply Box IX3 RFACA IX3 RFALFE UPFLN4x U FF filter cube TV1XC X Cite Turbo 19 Instructions for use OLYMPUS VS200 System...

Страница 20: ...0 ST23 VS200 SlideTray 2 x3 VS20 ST34 VS200 SlideTray 3 x4 VS20 ST45 VS200 SlideTray 4 x5 7 Fluorescence Cameras Fluorescence Solution Additional Tray Solutions Additional Objective Solutions VS200 sy...

Страница 21: ...nal Filter sets Component A B C D Recommended filter set single band exciter multi band emit ter single band exciter single band emit ter single band exciter single band emit ter multi band emit ter M...

Страница 22: ...200 Tray types The VS200 systems can be equipped with four different tray types for different slide formats All trays can be either used with the VS200 ST Single Tray Load system or VS200 MTL Multi Tr...

Страница 23: ...7 Trays Additional tray types 3 slides in 2x3 inch 76 x 52 mm 1 slide in 3x4 inch 76 3 x 103 mm 1 slide in 4x5 inch 76 3 x 128 mm Add image 23 Instructions for use OLYMPUS VS200 System...

Страница 24: ...17 mm Optical frame Illuminator Built in Koehler illumination for transmitted light Objectives Compatible objectives 2x 4x 10x 20x 30x 40x 60x and 100x selected oil and silicon immersion objectives Ph...

Страница 25: ...Software Image format vsi JPEG JP2 TIFF zooming while scanning annotations automatic sample detec tion Z stack extended focus imaging screen cap ture step less zooming synchronized multi images displa...

Страница 26: ...anner or other components might drop when you are moving them Injury to your feet or damage to the device can result Make sure that all handles for transportation are screwed in completely and tight T...

Страница 27: ...ohne dass zuvor die Trans portsicherungen vollst ndig demontiert wurden kann es zu Ger tesch den kommen Demontieren Sie vor Inbetriebnahme des Scanners unbedingt alle Trans portsicherungen 9 2 1 Remov...

Страница 28: ...he customer the operations are not ensured ATTENTION The transportation locks must be remounted each time before the unit is trans ported Make absolutely sure not to lose the transportation locks and...

Страница 29: ...ansportation lock from the transmitted light condenser To do so remove the 2 hex screws size 3 indicated in the figure 3 Pull the transportation lock forwards and down to remove it 29 Instructions for...

Страница 30: ...ift the loader system onto a table 1 Remove the outer cardboard packaging and take all small boxes out of the package 2 Take the top panel back panel and the two front panels out of the boxes 3 Unpack...

Страница 31: ...tape stripes for the grounding flags as well as the tape that secures the front door 10 Remove the tape at the bottom left corner and take out the bubble foil pocket which contains the cable for the l...

Страница 32: ...itor The units described below must be assembled and adjusted by Olympus If these units are assembled or adjusted by the customer the operations are not ensured 1 Take the PC out of it s cardboard pac...

Страница 33: ...nahme des Loaders unbedingt alle Trans portsicherungen ATTENTION The transportation locks must be remounted each time before the unit is trans ported Make absolutely sure not to lose the transportatio...

Страница 34: ...size 3 mm 1 Remove the red transportation lock from the VS200 loader s tray hotel To do so loosen the 4 hex screws size 3 mm indicated in the figure 9 4 2 Removing transportation lock from SCARA robot...

Страница 35: ...river size 3 mm 1 Remove the red two part transportation locks from the SCARA robot arm To do so loosen the 8 hex screws size 3 mm indicated in the figure 9 4 3 Removing transportation lock from SCARA...

Страница 36: ...ting material Hex screwdriver size 3 mm 1 Remove the red transportation lock from the brass colored counterweight of the SCARA robot arm To do this loosen the 3 hex screws size 3 mm indicated in the f...

Страница 37: ...tives should be mounted into fixed positions as per the list below You can rotate the nosepiece revolver in any direction so that it is easier to mount an objective By default a VS200 system is shippe...

Страница 38: ...ation methods In this case refer to chapter Device settings objectives auf Seite 87 and Device customization auf Seite 90 If you have a brightfield system only please continue with chapter Assembly of...

Страница 39: ...stem can be equipped with an automatic liquid dispenser capable of dispensing different types of immersion medium Once a particlular type of immer sion medium has been used you can no longer switch to...

Страница 40: ...ed to setup fluorescence for the VS200 system Carefully unbox all components and fol low the instructions 11 1 IX3 RFACA and IX3 RFALFE The units described below must be assembled and adjusted by Olym...

Страница 41: ...s pushed in completely 4 Goto the scanner and open the front door 5 Remove the six 2 5 mm hex socket screws which hold the black tamper pro tection plate like shown in the image below 6 Remove the two...

Страница 42: ...g bracket which is attached to the frame at the back of the system 9 Insert the IX3 RFACA IX3 RFALFE unit into the system Make sure that it fits into the rails on the left and the right side and flip...

Страница 43: ...hten the two 3 mm hex socket screws to fix the brackets 11 Attach the RFALFE supporting bracket again at the back of the system s frame Insert the two 4 mm hex socket screws but don t tighten them 43...

Страница 44: ...et screws 13 Make sure the power is switched off and attach the IX3 RFACA cable to the socket at the back of the IX3 RFACA 14 Attach the black tamper protection plate again and tighten the six hex soc...

Страница 45: ...he customer the operations are not ensured Depending on the customer s configuration you either have to mount only one or two fast filter wheels The following chapter describes how to mount the differ...

Страница 46: ...FITC 3 CY3 4 CY5 5 CY7 6 Black out filter 7 Black out filter 8 Black out filter Refer to the U FFWR instruction manual to the chapter for filter insertion for more information The orientation for Sem...

Страница 47: ...U FFWO connect the terminator to the second plug at the U FFWR 11 2 2 Add or replace filter U FFWR 1 Make sure that the power of the VS200 system is switched off 2 Remove the backside top panel See T...

Страница 48: ...as you will need it later 6 Configure the filter in the VS200 ASW software To do this open the Device Settings dialog box You can open this dialog box in the Manual control layout 7 Click the Previous...

Страница 49: ...its are assembled or adjusted by the customer the operations are not ensured Hex key 2 0 to lock the tubus Hex key 3 0 to open the filterwheel Small spanner to adjust the height of the tubus Spanner t...

Страница 50: ...on 25 mm emission filter 1 DAPI 432 36 2 FITC 515 30 3 CY3 595 31 4 CY5 680 42 5 CY7 6 Black out filter 7 Black out filter 8 25 mm Glass 2 Refer to the U FFWO instruction manual to chapter Inserting t...

Страница 51: ...1 2 4 Add or replace filter U FFWO Hex key 3 mm 1 Make sure that the power of the VS200 system is switched off 2 To add or replace a 25 mm emission filter in the U FFWO open the cover of the filter wh...

Страница 52: ...nav igation bar on the VS200 ASW software s start page 7 At the top right on the menu bar click the Manual control button 8 Select the Acquire Devices Device Settings command to open the Device Settin...

Страница 53: ...monochrome camera auf Seite 53 for the ori entation of the camera 11 3 1 Orientation of the monochrome camera The units described below must be assembled and adjusted by Olympus If these units are as...

Страница 54: ...e attachment of VS304M iDS and ORCA Fusion VS200 is identical Mounting a camera to the U FFWO Refer to the U FFWO instruction manual to chapter Mounting a camera to the U FFWO for more information 1 C...

Страница 55: ...the large wrench tool as indicated below 1 fasten clockwise 2 large wrench small side 4 Hand tighten the camera and C mount adapter to the U FFWO Be aware to tighten the camera orientation lock first...

Страница 56: ...justment How to adjust the camera adapter tubus is described in chapter Camera adapter auf Seite 139 11 4 X Cite adapter The units described below must be assembled and adjusted by Olympus If these un...

Страница 57: ...e overview images the UPFLN4X has to be mounted on position 6 of the IX3 nosepiece See Mounting of components auf Seite 37 for installation instructions 11 6 LED light source The units described below...

Страница 58: ...Image 3 Insert the other end of the light guide into the LED light source see man ufacturer s operating manual for detailed information 11 7 Filter set The units described below must be assembled and...

Страница 59: ...ace filter cube IX3 1 Make sure that the power of the VS200 system is switched off 2 To add or replace an IX3 filter cube into the IX3 RFACA slide the door of the IX3 RFACA to the left 3 Insert the fi...

Страница 60: ...placed into position 8 of the IX3 RFACA because this position is configured in the default device settings 1 Insert the U FDICT into position 8 as shown in the image It is between the index 5 and 6 R...

Страница 61: ...e units are assembled or adjusted by the customer the operations are not ensured The excitation filters in the X Cite Turbo have to be placed as indicated in the boxes in the picture below the arrows...

Страница 62: ...dles Follow the correct order for mounting the elements of the housing so that you don t run into any trouble 12 1 Left panel The units described below must be assembled and adjusted by Olympus If the...

Страница 63: ...locks sockets 1 at the top and two small pins 2 close to the bottom as well as two grounding flag connectors with cables attached 3 1 Place the panel next to the system and connect the two grounding...

Страница 64: ...ng of the right side panel is similar to the left panel There are two rubber sockets quick locks at the top and two small pins close to the bottom of the VS200 scanner and three grounding flags 1 Firs...

Страница 65: ...ting the top panels It is fixed with four knurled head screws The three knurled head screws at the top are part of the top panel and are tightened later 1 Guide the three cables from the backside of t...

Страница 66: ...front and two guiding holes in the back 2 Additionally there are two grounding connectors 3 where you have to connect the grounding flags coming from the VS200 frame On each side of the VS200 scanner...

Страница 67: ...this Mount the beam splitter cover In case you have a brightfield system only you have to mount the beam splitter cover now top view bottom view 1 Put the round cover on top of the beam splitter flang...

Страница 68: ...ock pins 2 1 To make the assembly easier connect the two grounding flags already to the grounding plugs 3 of the top panel 2 Open the front door and put the short top panel parallel on the scanner 3 G...

Страница 69: ...uided through the housing like in the image below If the top panels are mounted correctly it should look like this 5 Connect the two grounding flags to their counter plugs on the right and left side l...

Страница 70: ...les It is important to connect all cables according to the connection scheme as otherwise components might not work correctly Please refer to the following table Cable Type Location PC position VS 364...

Страница 71: ...Xylis Power cord Back of Xylis U FFWR CAN Bus VS200 internal See chapter Fluor escence filter wheels or camera adapter for monochrome camera auf Seite 45 U FFWO CAN Bus VS200 internal HP LCD screen Di...

Страница 72: ...re not ensured 1 Place the top panel over the system 1 Attach the two grounding flags at the backside of the loader to the con nectors of the panel like shown in the image below 2 Position the panel s...

Страница 73: ...embled or adjusted by the customer the operations are not ensured 1 Mount the horizontal and vertical front panel as described in the following steps Before After 2 Connect the two grounding flags to...

Страница 74: ...g to the vertical panel and push the panel towards the system so that the quick lock sockets of the panel fit onto the quick lock pins of the loader 74 Instructions for use 14 Assembly of the loader h...

Страница 75: ...and the VS200 loader Hex screwdriver size 5 mm 1 Switch the VS200 system off using the main power switch and disconnect the system from the power supply 2 Place the VS200 loader to the right of the VS...

Страница 76: ...size 5 mm which attach the connector of the VS200 scanner to the VS200 loader 8 Remove the remaining handles from the VS200 scanner and the VS200 loader 9 Reconnect the VS200 system to the power suppl...

Страница 77: ...to change the language of the operating system follow the instructions below Japanese and Chinese are pre installed on the VS200 PC 1 Open the Windows Settings and select Time Language 2 Click on the...

Страница 78: ...chapter describes the installation of VS200 ASW on a VS200 system PC 1 Turn on the PC 2 To start the setup process go to D OLYMPUS_SERVICE_ONLY_DO_NOT_ DELETE SetupMain and run the Setup exe 3 Click Y...

Страница 79: ...he configuration of your kit Available license keys are VS200 ASW 3 1 VS FLUO VS CNV 6 Accept the license agreement To activate the licences at customer site the PC needs internet access If no interne...

Страница 80: ...oceed with Next 8 Change installation location not recommended Proceed with Next 9 A change of the temporary file location D Temp is not recommended Proceed with Next 80 Instructions for use 17 VS200...

Страница 81: ...e camera than the IDS VS 304M select either ORCA Flash4 0 or ORCA Fusion VS200 and proceed with Next 11 Select Add icon to the desktop and Add manuals shortcut to the desktop and proceed with Next 12...

Страница 82: ...y clicking the Finish button 14 In the following dialog box click the Close button and do not install any example data or tools 15 Turn on the VS200 system 82 Instructions for use 17 VS200 ASW softwar...

Страница 83: ...ent and proceed with Continue The following splash screen is displayed while the VS200 system is ini tializing The initialization process might need up to two minutes Error messages If you see the err...

Страница 84: ...nge or adapt the VS200 device configuration If you see the following error message it means that you are using a VS200 loader which is not yet configured in the device list Refer to chapter VS200 devi...

Страница 85: ...2 In the Device Settings dialog box click on the Device List button to open the Device List dialog box 3 VS200 ASW offers five predefined device configurations to choose from Depending on the configu...

Страница 86: ...polarizer is switched off 1 Use the Acquire Devices Device List command to open the Device List dialog box 2 Select the Transmitted light path tab and select the Polarization Angle Changer check box...

Страница 87: ...ously Used Layout button at the top right in the nav igation bar on the software s start page 2 At the top right on the menu bar click the Manual control button 3 Select the Acquire Devices Device Set...

Страница 88: ...s dialog box 1 Use the Previously Used Layout button to go to a different layout You can find the Previously Used Layout button at the top right in the nav igation bar on the VS200 ASW software s star...

Страница 89: ...riate position and selecting the name of the filter 5 Click the OK button to save the changes 6 Subsequently you have to configure an observation method in the Device Customization dialog box to be ab...

Страница 90: ...n the Device Settings dialog box To open the Device Settings dialog box use the Acquire Devices Device Settings command As none of the ORCA cameras are part of the default device configurations each o...

Страница 91: ...the BFMono observation method OM 3 Unlock the BFMono observation method by clicking on the lock icon 4 In the Status picklist set the Orca camera to the status Use 5 In the Image type picklist change...

Страница 92: ...ire Devices Device Customization command to open the Device Customization dialog box 2 Select the BFMono observation method 3 Use the Copy Observation Method button to make a copy of the observation m...

Страница 93: ...to the correct magnification Objective magnification BX3 condenser PH insert 10x U PH1 S 20x U PH1 S 40x U PH2 S 60x U PH3 S 100x U PH3 S 8 Select the BX3 Top Lens entry from the Available components...

Страница 94: ...x select the BF observation method 3 Use the Copy Observation Method button to make a copy of the observation method 4 Type in a name e g Polarization 5 In the Type picklist select Polarized 6 Select...

Страница 95: ...on method OM e g for fluorescence image acquisition is to copy an existing one and adjust the settings according to your needs in the Device Customization dialog box Open the Device Customization dial...

Страница 96: ...ts list select the Fluorochromes entry 5 Select the Use entry from the Status list 6 In the Fluorochrome list select the fluorochrome that fits to your new filter combination 7 In the Available Compon...

Страница 97: ...20x UPlanXApo 0 5 not defined 40x UPlanXApo 0 7 not defined 40xUplanXApoS 60x UPlanXApoO not defined 100x UPlanXApoO not defined 18 7 2 Orca Fusion voltages Observation method Objective BFMono AS 75...

Страница 98: ...lanXApoO not defined 100 100x UPlanXApoO not defined 100 18 7 4 VS 264C voltages Observation method Objective BF AS 75 DF AS max POL AS 75 2x PLN 60 not defined 100 4x UPLFLN 100 100 10x UPlanXApo 65...

Страница 99: ...ays in den VS200 Scanner Durch den motorisierten Antrieb der Einschubklappe am Scanner besteht Quetschgefahr H nde und Finger k nnen beim Einsetzen der Trays gequetscht werden Achten Sie darauf dass k...

Страница 100: ...and insert the tray horizontally following the ori entation which is printed on the tray Insert this way into an empty pos ition 3 Push it all the way in until the tray indicator LED on the left side...

Страница 101: ...ion wizard for all necessary cal ibrations Theimageshows the Calibrations dialog box In order to get optimal results we recommend using the VS Olympus Calibration Slide v2 0 to calibrate the system La...

Страница 102: ...lick the Select slide for calibration button 4 Load the tray either manually or using the loader by clicking the Load Slide and Calibrate button The software switches to the Manual control layout 5 In...

Страница 103: ...in However the Z axis calibration is dependent on the glass slide thickness Therefore it is neces sary to calibrate the Z axis 1 In the Calibrations dialog box select the Stage Limits entry and click...

Страница 104: ...utton to switch to live mode 7 Use the stage navigator to move to the center of the slide 8 Use the Ctrl mouse wheel combination to focus the sample If you have trouble getting the image into focus us...

Страница 105: ...20 Calibrate VS200 using the Olympus Calibration Slide 10 Click the Finish button to finalize the calibration process 105 Instructions for use OLYMPUS VS200 System...

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