38
CASY TT Operator‘s Guide
Preparing Samples
6. PERFORMING AND EVALUATING
MEASUREMENTS
Working with your CASY System is very simple. The system is ready to start a measurement as
soon as it is switched on. However, please observe the following before you start to measure your
samples:
+
On initial start-up or after moving the system, check the points cited in Chapter 3.1 (
Choosing the
Location
) and Chapter 3.2 (
Cabling
).
+
Follow the steps described in Chapter 3.4 (
Preparing for Measurement
). Specifically:
+
Fitting (see Chapter 2.2) and selecting (see Chapter 5.5) the required measuring capillary
where necessary.
+
Installing the protection shield (see Chapter 3.4 and the
CASY TT Customer Training Guide
)
+
Selecting an appropriate setup (see Chapter 5.3) or manually inputting the para meters (see
Chapter 5.4).
+
Background monitoring (see Chapter 3.4).
Please note that after power-up, the system will automatically load the previously used setup for the
previously used measuring capillary. Further information on how to manually specify parameters or
create a new setup can be found in Chapter 6.5.
6.1 Preparing Samples
+
Fill a CASY cup with 10 ml filtered CASY ton.
+
Add an aliquot of the cell suspension to be examined (normally between 10 and 200 µl).
+
Secure the lid and mix the sample by tilting the CASY cup three times. Carefully avoid the forma-
tion of bubbles or foam!
+
Your CASY System can measure only the samples you provide. The quality of the measuring
result depends on how well you prepare your samples. Standardize the sample pre paration pro-
cedure as far as possible (used cell culture media, timing of sample preparation, duration and
intensity of cell re-suspension, carrying out of the required dilution steps etc.)
+
You should always freshly prepare your samples just before the measurement. Depending on
the sensitivity of the cell type, there is no guarantee that your cells in CASY ton will remain
unchanged in terms of cell count, cell volume, aggregation and viability over long periods. If a
sample remains in CASY ton for longer than two minutes, you must remix it before performing
the measurement.
+
Always use new CASY cups. This ensures that your measuring results are affected by high back-
ground caused by contaminated measuring cups.
