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CASY TT Operator‘s Guide
1.2.2 Pulse Area Analysis
The measuring signal, for example the electrical pulse generated by a cell when passing the
measuring pore, is scanned by the CASY Cell Counter and Analyzer at high frequency (1 MHz). As
well as the amplitude of the measuring signal, it also records the shape and time course of the signal
as a whole (Figure 2).
The CASY Cell Counter and Analyzer calculates the integral of the measuring signal from the individ-
ual readings. The calculated signal areas are evaluated by a standardized method and accumulated
in a multi-channel analyzer. From the original volume-linear size distribution with a resolution of
524,288 measuring channels finally a diameter-linear size distribution with a resolution of 400
display channels is calculated.
Electrodes
Measuring pore
1 million measures/sec.
Assigned in 512.00 channels
⇒
Signal analysis in area,
height, width and course
of time
Signal Analysis
Figure 2: Pulse Area Analysis.
Measuring Principle
Pulse Area Analysis
