31
If ambient temperature differs from 23°C of more than 2°C, consider calibrating the chamber
as described in the next paragraph.
12
Temperature control mechanism in custom configuration at 37.0°C
The goal is to identify the correct offset (i.e. increase or decrease the controlled base plate and
transparent lid temperature manually) of Base and Lid of the chamber, so that the sample
cultured in a 35 mm Petri Dish, is at the desired temperature (eg. 37.0 °C). By measuring the
sample temperature with the Free Sensor (Fine Gauge Thermocouple), during the steady
state, the offset values can be estimated, as described below:
1.
Plug the green Fine Gauge Thermocouple in the port labeled “Reference Temperature”
located in the rear panel of the Temperature Control Unit, as illustrated in Figure 14.
2.
Insert the Fine Gauge Thermocouple into the chamber by threading the wire through
one of the perfusion holes on the chamber riser.
3.
Secure the end of the Fine Gauge Thermocouple to the bottom of a 35mm or similar
Petri dish using some tape making sure to avoid covering the head of the probe (see
Figure 16. Free Sensor attached on the internal base of the Petri Dish.
4.
Fill the Petri dish with distilled water to a level sufficient to fully immerse the Fine Gauge
Thermocouple. This is a Reference Well simulating experimental conditions. Place the lid
on the Petri dish, and insert the dish in the incubation chamber. You can also use
SENSOR LID-# to close the dish (see the “Tip” below).
Tip
►
It is recommended using
an
Okolab SENSOR LID (available for purchase) to secure the
Fine Gauge Thermocouple in place in the Reference Well during calibration (See Figure 17).
Sensor lids are available for the following: 35 mm, 60 mm Petri-dish, chamber slide and
chambered cover glass. Sensor Lids are ordered separately using the part numbers in Table 2
below.
