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Preface

UNO-COMBINED-CONTROLLER together with a stage top Electric chamber (preventing CO2

of the desired concentration from dissipating away from the sample) is a compact solution for

long term time lapse imaging. We will refer to UNO-COMBINED–CONTROLLER as ‘UNO’ from

here on. ‘UNO’ is designed to maintain all the required thermal and gas conditions for keeping

cells alive for the duration of the time lapse imaging session. “UNO” has a temperature control

range of 25-50°C. The system includes a Temperature Control Unit, a manual Gas Mixer -with

floating ball flow meters-, and a passive Humidity Module with its own heater. The UNO-

COMBINED-CONTROLLER can be connected to a stage top Electric Environmental Chamber

from here on referred to as ‘Incubator’. As OPTIONS a fine gauge thermocouple can be

added to monitor the temperature of a Reference Well located in close proximity to the

experimental one. An Objective Heater is REQUIRED when using oil immersion objectives.

Additionally, UNO-COMBINED-CONTROLLER can be equipped with an optional air pump: OKO-

AP, which is a plug and play solution for Air inlet and a convenient alternative to 100% Air

tanks/compressed Air lines. All the heated components of the ‘Incubator’ are heated by 24V

DC and their temperatures monitored by High-Accuracy thermistors. Temperature sensors with

0.1°C accuracy are embedded into the following components: lid and base of the ‘Incubator’,

humidity module and objective-heating collar. A single thermal controller regulates the

temperature of the individual components above in a coordinated fashion avoiding

overshooting the target temperature. Each unit undergoes a careful factory calibration

guaranteeing that the sample temperature, inside the chamber, is kept at the desired value

within the range 25 – 50 °C. The system is equipped with visual and sound alarm alert in case

the temperature limit is reached. The unit shows current and set point temperature in the

same display. ‘UNO’ includes an integrated manual CO

mixer allowing regulating Carbon

Dioxide Concentration in Air within the range 1 – 20%. Before use please read this manual and

familiarize yourself with the functions and the operation of the device.

Содержание UNO-COMBINED-CONTROLLER

Страница 1: ...1 UNO COMBINED CONTROLLER CO2 Microscope Electric Top Stage Incubation System Manual Vers 03 16 ...

Страница 2: ...2 This page was left blank ...

Страница 3: ...er connection ports 15 Tubing Connections 16 Pressure Gauge set up and use 19 Assembling Instruction of the Bubbling Column 21 Cables connection 21 Incubation chamber 24 Fine gauge thermocouple Free Sensor optional 27 10 CONTROL UNIT FRONT PANEL 28 11 TEMPERATURE CONTROL MECHANISM IN STANDARD CONFIGURATION 29 12 TEMPERATURE CONTROL MECHANISM IN CUSTOM CONFIGURATION AT 37 0 C 31 13 UNIT OPERATION T...

Страница 4: ...47 UNO TS software Active Message 48 14 OBJECTIVE HEATER MANUAL CALIBRATION 48 15 FLOW METER REGULATION 49 16 MAINTENANCE 51 17 SUPPORT 52 Web Conference For Assistance And Training 52 Troubleshooting 53 Technical Support 54 ItalyTechnical Specifications 54 18 FIGURES 55 ...

Страница 5: ... 1 Revision table Revision Number Additions or changes Date Editor 01 Edited November 2014 Amelia Liccardo 02 Review March 2015 Silvia Foppiano Amelia Liccardo 03 Minor Review June 2016 Francesco Marino ...

Страница 6: ...INED CONTROLLER can be equipped with an optional air pump OKO AP which is a plug and play solution for Air inlet and a convenient alternative to 100 Air tanks compressed Air lines All the heated components of the Incubator are heated by 24V DC and their temperatures monitored by High Accuracy thermistors Temperature sensors with 0 1 C accuracy are embedded into the following components lid and bas...

Страница 7: ... product must not be disposed as urban solid waste This symbol indicates the protection degree against ingress of solids or liquids inside the product 4 Safety Notes Before operating the equipment please read carefully the instructions and the safety notes If you have any questions please contact Okolab The equipment must only be used as intended and as described in this Manual Technically qualifi...

Страница 8: ...rd objects as it may be damaged Power cord of unit should be unplugged from electrical outlet when left unused for long period of time VENTILATION please make sure the vents on the unit are not blocked in any way either partially or fully Unit should be situated away from heat sources such as radiators heat registers stoves or other appliances or processes that produce heat Do not start up the equ...

Страница 9: ...ries We reserve the right to make technical variations IN NO EVENT SHALL OKOLAB S R L BE LIABLE FOR ANY DIRECT INCIDENTAL OR CONSEQUENTIAL DAMAGES OF ANY NATURE FINANCIAL OR OTHER LOSSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE OF ANY PRODUCT 5 AC section wiring diagram Figure 1 shows the alternating current AC section designed to connect internal power supplies see Figure 1 label C in para...

Страница 10: ...mq BLUE 1mmq BROWN 1 5mm G V 1mm BLACK 1mm RED 5V DC 0V Power Supply TDK Lambda ZWS150 24 85 265V c a 24V c c 6 5 A Power Supply TDK Lambda ZWS5 5 85 265V c a 5V c c 1 A POSITIVE CLAMPS 24 V DC NEGATIVE CLAMPS 24V DC 0V 0V 0V 1 5mm G V 5V DC 0V 0V 0V M 24V DC 0 5mm BLACK 0 5mm RED BLUE RED 0V 0V 24V DC 0V A B B Figure 1 AC section wiring diagram ...

Страница 11: ...in Control Unit Humidifying Module with Humidifier Module Heater Incubating Chamber Objective Heater Optional When working with Premixed 5 CO2 remember connecting the rigid tube coming from pressure gauge to Air port labeled Air In on rear panel of the Control Unit In this working modality Okolab recommends setting a flow rate of 0 3 L min using the floating ball flow meter on the front Panel of t...

Страница 12: ...d to the Humidity Module 7 TUBE H 1 5 m long tube composed of 4mm ID silicon transparent tube 0 5 m long connected to 2mm ID silicon transparent tube 1 m long both wrapped in an insulating material Use insulated tubing in point 8 to connect the Humidity module to the Incubating chamber The following items are required but sold separately UNO must be equipped with 8 Incubating chamber model dependi...

Страница 13: ...dule A unique algorithm maintains these temperatures constant A careful calibration See Table 1 performed in our laboratories guarantees that sample temperature is maintained at the desired value by selecting the Standard Mode See paragraph 13 5 1 working modality An external Fine Gauge Thermocouple i e a thin flexible green wire temperature sensor optional can be used to read the temperature of a...

Страница 14: ...14 Humidity Regulation Humidifying and pre heating modules prevent medium evaporation and avoid water condensation on glass and plastic surfaces ...

Страница 15: ...efully all the instructions and safety notes Digital Temperature Controller connection ports 1 2 5 6 3 4 7 9 10 13 14 15 16 8 11 12 Figure 3 Control Unit Rear Panel Overview 1 CO2 and Air mixture output 2 RS232 serial port Software UNO TS can be used as optional to read incubator temperature and store the data in computer memory 3 R T Fine gauge thermocouple port 4 Time Lag Fuses 5x20 x2 see Power...

Страница 16: ...ying module heating connector 12 Fuse 3 1 6 A Fast Fuse 13 Obj Heater Objective Heater Connector 14 Fuse 4 1 6 A Fast Fuse 15 Pure Air or Premixed Air 5 CO2 Push to fit Input Connector for 6 mm O D rigid tubing 16 Pure CO2 Push to fit Input Connector for 6 mm O D rigid tubing Input Tubing Connections Figure 4 Complete gas lines scheme ...

Страница 17: ...lease contact Okolab Secure the Pressure Gauge for CO2 with regulator to the wall by using the supplied assembling stirrup supplied accessories A filter is added to the Pressure Gauge Filtering degree is 20μm CO2 stream will be FILTERED and at STABLE Pressure Do not fill the filtering container with oil Connect the rigid tube 6mm O D coming from the pressure regulator of the CO2 tank not supplied ...

Страница 18: ...t connector on the rear panel of the Control Unit to the Inlet barb connector on the Humidifier bottle as illustrated in Figure 4 and Figure 5 6 Use TUBE H supplied see paragraph 7 1 assembled as follows 0 5 m long 4mm ID silicon transparent tube 1 m long 2mm ID silicon transparent tube wrapped in insulating material to connect the Outlet barb connector on the Humidifier bottle to the miniature ba...

Страница 19: ...tion It is recommended to periodically check tubing connections and gas flow Pressure Gauge set up and use Follow the direction of arrow on the rear of the Pressure Gauge for the correct Gas In Out See Figure 7 below GAS IN GAS OUT Figure 7 Input and Output gas ports See symbol on the rear of Pressure Gauge On the bottom of the unit there is a purging valve Close it before operating the Pressure G...

Страница 20: ...close the gauge pull the knob up and rotate it counterclockwise Push the knob down to lock it Open valve on gas supply tank Adjust Pressure Gauge by pulling the knob up and rotating it clockwise until the pressure reaches the desired value See Figure 9 A B C Figure 9 Pressure gauge using ...

Страница 21: ...ure 11 in order to connect the cables to Control Unit 1 Connect the base of the incubation chamber red labeled to the port labeled Heater 1 on the rear panel of the Control Unit see Figure 11 2 Connect the lid of the incubation chamber yellow labeled to the port labeled Heater 2 on the rear panel of the Control Unit see Figure 11 3 Connect the humidifying module heater green labeled to the port la...

Страница 22: ... COMBINED CONTROLLER Base Incubating Chamber To PC Figure 11 Cables connection Standard configuration 7 NOTE an Objective Heater not included purchased separately is required when using oil immersion objectives The Objective Band Heater kit is designed to fit around any type of microscope objective for upright and inverted scopes It includes three Heating Bands and three Velcro tapes of different ...

Страница 23: ...bjective cover it with the Velcro tape and fix the Velcro Tape as illustrated in Figure 13 Then install the Objective to the microscope nosepiece rotate the Objective carefully avoiding damaging the wires or the flexible heating band Avoid holding the Objective Heater Band by lead wires Do not modify or cut in any way the Objective Heating Band in the attempt to fit objective of different size Mak...

Страница 24: ...according to the incubating chamber model and sample adapter you have ordered You can see the current base lid and HM offsets on the display See paragraph 13 5 1 Note Thermal control settings are a function of the adapter in use Hence every time you change adapter you must input the correct thermal settings using the procedure described in paragraph 13 5 1 In case you purchase additional incubatin...

Страница 25: ...M offsets are automatically calculated when you insert the base offset If your sample adapter is not present in Table 1 you can use the Custom configuration and calibrate the chamber in your lab See paragraph 13 5 2 Tip For a list of all available models and specs of each chamber and adapter see www oko lab com ...

Страница 26: ...LABTEK II 35 M LABTEK 35 NZ500 LABTEK II 35 NZ500 GS35 PRIOR NZ500 2 9 H301 NIKON TI SR H301 OLYMPUS IX SUSP 1x35 M single 1x60 single 1 4 1xGS M single 1xLABTEK M single 1xLABTEK II M single 2 6 H301 EC LG BL H301 EC LG 1x35 H301 EC LG 1xGS H301 EC LG 1x60 3 0 H301 EC LG UP BL H301 EC LG UP 1x35 2 2 H301 EC LG UP 1x60 1 9 H301 EC LG UP 1xGS H301 EC LG UP 1xLABTEK H301 EC LG UP 1xLABTEK II 2 1 H30...

Страница 27: ...e incubating system can be equipped with an external Free Sensor Fine Gauge Thermocouple Optional added to the system with the purchase of UNO TS software which allows measuring the temperature inside a reference well Use the Fine Gauge Thermocouple to calibrate the chamber s temperature or simply to check the specimen temperature once the steady state is reached ...

Страница 28: ...re of the chamber s base plate plus the reading offset 2 LID is the actual temperature of the chamber s transparent heated lid plus the reading offset 3 HM is the actual temperature of the Humidity module plus the reading offset Tip The HM temperature appears only if the HM is active see paragraph 13 7 4 OBH is the actual temperature of the Objective Heater plus the reading offset Tip The Objectiv...

Страница 29: ... reads and controls the temperatures of the incubation chamber s parts adding a reading offset to the BASE LID and HM temperatures in order to have the sample at the set point temperature To show the offsets on the display select Offset Mode as Standard Enter the Offset Submenu see paragraph 13 5 1 Reading Offsets were evaluated with a careful calibration performed by Okolab at ambient temperature...

Страница 30: ...so allows maintaining the sample temperature at the Set Point Temperature without the need of any additional calibration The Reading Offset values vary with the Set Point temperature and with the chamber and sample adapter in use Advantages of this approach The incubating system is Easy to use Fast experiment start up Well suited for multi users Sample temperature accuracy of 0 3 C from the Set Po...

Страница 31: ...uple into the chamber by threading the wire through one of the perfusion holes on the chamber riser 3 Secure the end of the Fine Gauge Thermocouple to the bottom of a 35mm or similar Petri dish using some tape making sure to avoid covering the head of the probe see Figure 16 Figure 16 Free Sensor attached on the internal base of the Petri Dish 4 Fill the Petri dish with distilled water to a level ...

Страница 32: ...ning system in the room from the incubation system See paragraph 13 5 2 for instructions on how to use the push button display to calibrate the chamber From the main screen press the Menu key on the instrument panel Press move the cursor using the up and down arrows to Offset mode and press Enter Press Enter with the cursor on Mode Press Up down until you see Custom Press Enter to select Custom as...

Страница 33: ...he Reading Offset of Lid temperature Tip Set the Offset of the Lid at a value higher than the Offset of the Base by 2 C Eg If offset of the Base is 5 C then set offset of Lid to 7 C As a result the Lid will be at a temperature 2 C higher than the Base This is done to avoid water condensation on the observation window With these assumptions the ONLY parameter that needs to be experimentally identif...

Страница 34: ... temperature differs more than 2 C from factory calibration temperature of 23 C Offset values are valid only for one Set Point temperature The Offset values must be experimentally re evaluated for each different set point If the sample adapter changes please modify the Offset and re calibrate the chamber to ensure that the sample will be at the correct temperature Offset values are evaluated accor...

Страница 35: ...cal or logical value is flashing Press down or up to move the cursor along the Main Menu items Press down or up to move the cursor along the parameters of a Sub Menu Press up to silence for 30 seconds the audible alarm when active Enter button Press Enter to have access to a Sub Menu from the Main Menu Press Enter after modifying a value to confirm it Press Enter to change the set point temperatur...

Страница 36: ...et Mode Offset Objective Heater Menu 2 3 àHumidity Module Alarm Settings Screen Settings â Go to main Page Menu 3 3 àProduct Info â Figure 19 Main Page and Main Menu Navigation Description To enter the configuration Main Menu Press Menu button Press Up or Down to advance navigate to the next menu item To Exit from the Main Menu press Menu button ...

Страница 37: ... temperature Main Page can display temperature of a Free Sensor Optional Free Sensor can be used to monitor the temperature inside the Petri dish to calibrate the chamber and to check the sample temperature during an experiment Figure 21 shows the Main Page when working with Free Sensor connected to the Control Unit Set Point 37 0 C Free Sensor 37 0 C Base 37 0 HM 37 0 Lid 37 0 OBH 37 0 Figure 21 ...

Страница 38: ...the incubating chamber model and sample adapter you have ordered and in standard working mode You can read the base offset in use on the display See Figure 22 You can also see all the current offsets lid base and HM offsets in Offset submenu See Figure 23 Set Point 37 0 C Base 37 0 HM 37 0 Lid 37 0 OBH 37 0 Menu 1 3 àOffset Mode Offset Objective Heater â Go to main Page Menu 1 3 Offset Mode àOffse...

Страница 39: ...Tip Keep in mind that thermal control settings change when changing the adapter in use Hence every time you change adapter you must input the correct base offset using the procedure of Figure 24 and the offset values reported in Table 1 In case you purchase additional incubating chamber models make sure to input the correct base offset prior to your experiment Menu 1 3 àOffset Mode Offset Objectiv...

Страница 40: ...ting the chamber following the procedure reported in paragraph 12 Menu 1 3 àOffset Mode Offset Objective Heater 8 Offset Mode àMode Custom Ref 37 C 1 3 Offset Mode Mode Custom 8 Enter to confirm Exit 8 Figure 25 How to choose the Custom Offset Mode From the main menu press the Menu key on the front panel Press Enter with the cursor on Offset mode Press Enter with the cursor on Mode Press Up down u...

Страница 41: ...tive Heater 1 2 àEnabled OFF Offset 0 0 Ramp C min 0 5 Change the value Exit 8 Confirm new value â á 8 Select the value to be changed â á Move the cursor to the Parameter to be changed Figure 27 Objective Heater Settings Menu From the main menu press the Menu key on the front panel Move the cursor to the item Objective Heater with the Arrow Keys Press Enter with the cursor on the item Objective He...

Страница 42: ...eset to factory 8 Click on Reset To Factory Reset to factory ready to be changed with flashing Item Confirm or Exit 8 â Figure 29 Reset to factory default In this Submenu the User can set a manual offset for Objective Heater as described in Figure 30 and following the procedure described in paragraph 14 Menu 1 2 Select an Adapter Offset àObjective Heater 8 Objective Heater 1 2 Enabled OFF àOffset ...

Страница 43: ...e Ramp Rate Value see Figure 31 Menu 1 2 Select an Adapter Offset àObjective Heater 8 Objective Heater 1 2 Enabled OFF Offset 0 0 àRamp C min 0 5 8 Enter to change the Ramp Value Confirm or Exit 8 â á Up Down until to desired Ramp Value Figure 31 Ramp rate changing From the main menu press the Menu key on the front panel Move the cursor to Objective Heater using the Arrow Keys Press Enter Move the...

Страница 44: ...dity Module Enabling From the main menu press the Menu key on the front panel Move the cursor to Humidity Module using the Arrow Keys Press Enter Press Enter with the cursor on Enabled and then press the Up Down Arrow keys until you see On Press Enter to confirm Press the Menu key TWICE to return to Main page When the Humidity Module is disabled the main page display will look as in Figure 36 Set ...

Страница 45: ...er turning the device on or right after changing the set point temperature the Alarm will be in standby for 30 minutes allowing the incubator to reach stable conditions After 30 minutes the Alarm will activate and start operating The Alarm function will check for alarm conditions An exclamation mark will appear next to the component of the chamber with temperature different from the set point more...

Страница 46: ...creen Settings Submenu Menu 2 3 Humidity Module Alarm Settings àScreen Settings 8 Screen Settings àContrast 50 Brightness 08 Reset to factory Change the value Exit 8 Confirm new value â á Select the value to be changed â á Move the cursor to the Parameter to be changed Figure 37 Screen Settings Menu The Screen Settings Sub Menu enables the operator to adjust the instrument screen contrast and brig...

Страница 47: ... the controller From the main menu press the Menu key on the front panel Move the cursor to Product Info using the Arrow Keys Press Enter Stop Mode Controller in Stop mode Click Enter to Exit Set Point 37 0 C Base 37 0 HM 37 0 Lid 37 0 OBH 37 0 Exit 8 8 Press Menu and Enter together Figure 39 Stop Mode display In the Run Mode press Menu and Enter at the same time to enable Standby Mode The control...

Страница 48: ...rature e g 37 0 C once the oil immersion objective is in contact with the bottom of the well This can be achieved measuring the sample temperature with the Fine Gauge Thermocouple during steady state and estimating the offset values as follows 1 Plug the green Fine Gauge Thermocouple into the port labeled Reference Temperature located in the rear panel of the Control Unit as illustrated in Figure ...

Страница 49: ...ends starting with an offset value corresponding to the difference between the set point temperature and the actual temperature measured by the Fine Gauge Thermocouple Press Enter and wait until the temperature measured by the Fine Gauge Thermocouple is stable If the temperature measured by the Fine Gauge Thermocouple is now close to the set point then the Manual Calibration is complete If the tem...

Страница 50: ...ween CO2 consumption condensation normally it decreases if Total gas stream flow rate increases and medium evaporation normally it decreases if Total gas stream flow rate decreases In the example below Figure 44 the gas flows on the second line in Table 4 are shown Set the flow meters keeping in mind that the correct position of the indicator floating ball is about in its middle The gas stream mix...

Страница 51: ...t Before cleaning the unit disconnect the unit from the power source Keep away from water Never use thinners benzene solvents on or near the devices since these could corrode their surfaces To clean the Stage Incubator and the Humidifying Module if it is present you can use distilled water or alcohol Verify the status of all cables and if some cable is damaged contact Okolab to receive assistance ...

Страница 52: ...llow these guidelines to facilitate setting up your web support Webcam installation instruction and Cdrom included Updated Skype software installed www skype com Register yourself on www skype com to have an account Skype_ID Set the audio and video and test them using Skype Contact our technical support sibillo oko lab com by e mail to set an appointment for web assistance ...

Страница 53: ...o receive assistance Err_4 Temperature read by Thermal plate sensor too high or too low Contact Okolab to receive assistance Err_5 Board Error Contact Okolab to receive assistance Err_6 Generic error Contact Okolab to receive assistance Sample temperature differs from the set point Ambient temperature too low or too high Regulate the ambient temperature Change the Offset value manually Chamber Bas...

Страница 54: ...BINED CONTROLLER Technical Specifications Temperature Chamber Temperature Range 25 50 C Sample Temperature range 3 C above ambient temperature to 50 C Step size 0 1 C Accuracy 0 3 C if room temperature remains within 1 C Regime temperature time about 30 min Operating Temperature 0 C 55 C Storage Temperature 5 C 60 C Operating Humidity 0 70 Power Consumption 115V AC 60 Hz Fuse 2 5 AT 250V 5x20 230V...

Страница 55: ... 35 mm Petri dish 32 Figure 18 Controller Display Main Page and function buttons 34 Figure 19 Main Page and Main Menu Navigation Description 36 Figure 20 Main Menu Display Changing Set Points Description 37 Figure 21 Main Page when working with Free Sensor connected to the Control Unit 37 Figure 22 Offset Mode Selecting 38 Figure 23 How to see the current offsets in standard mode 38 Figure 24 How ...

Страница 56: ... that result from operation not according to the instructions listed in the operation manual This warranty does not cover or involve any other equipment that may be used along with the Okolab System i e mini incubators any gas tank etc whose usage should be considered independent and performed according to their own operational instructions Okolab S r l warrants only the parts manufactured by it w...

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