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OM0302

Rev 2/December 2020

Page 62 of 107

Alternate Procedure:

The alternative procedure to determine inflow velocity uses a thermo anemometer in a constricted window access

opening of 3 inches (76mm) with the armrest removed. Inflow air velocity is measured in the center of the constricted

opening 1-1/2 inches (38mm) above the work access opening on the following specified grid. Use the correction factor

table to calculate the inflow velocity.

c. Test Data - Inches (mm):

1. DIM Measurement

Inflow Volume

ft.

/min.(m

/s)

Access Opening

ft.

)

Inflow Velocity

ft./min(m/s)

2. Constricted 3 inch (76mm) high access opening measurement - Inches (mm):

400

(102)

8.264

(210)

12.528

(318)

16.792

(426)

21.056

(535)

25.320

(643)

29.584

(751)

33.848

(860)

38.112

(968)

42.375

(1076)

500

(102)

8.198

(208)

12.396

(315)

16.594

(421)

20.792

(528)

24.990

(635)

29.188

(741)

33.386

(848)

37.584

(955)

41.782

(1061)

45.980

(1168)

50.178

(1274)

54.375

(1381)

600

(102)

8.158

(207)

12.316

(313)

16.474

(481)

20.632

(524)

24.790

(630)

28.948

(735)

33.106

(841)

37.264

(946)

41.422

(1052)

45.580

(1158)

49.738

(1263)

53.896

(1369)

58.054

(1475)

62.212

(1580)

66.375

(1686)

Number of Readings:

Average Velocity of Constricted Area

ft./min.(ms)

Average Velocity of Constricted Area

fpm (m/s)

2. X Constricted Access Area

)

3. = Constricted Area Volume

CFM(m

/s)

Constricted Area Volume

CFM(m

/s)

10”(254) or 8” (203) Access Window Area

)

6. = Average Velocity of 10”(254) or 8” (203) Access Window Area

fpm (m/s)

Average Velocity of

10”(254) or 8” (203)

Access Window Area

fpm (m/s)

8. X Correction Factor for Window Height

9. = Average Inflow Velocity

fpm (m/s)

d. Acceptance Criteria: Access Opening Inflow Velocity = 100 to 110 fpm (.51 to .56 m/s)

Areas/Correction Factors for Calculations

Cabinet Size

Window Access Opening

Correction Factor For

3" (76mm)

Constricted

10" (305mm)

8" (305mm)

10"(305mm)

Window

8"(305mm)

Window

400

.97

(.090)

3.22

(.299)

2.58

(.240)

1.21

1.20

500

1.22

(.113)

4.05

(.376)

3.24

(.301)

1.20

1.18

600

1.47

(.137)

4.89

(.454)

3.91

(.363)

1.15

1.18

Содержание LabGard NU-581-400

Страница 1: ...inar Flow Biosafety Cabinet Models NU 581 400 500 600 Bench Console Operation and Maintenance Manual December 2020 Revision 2 Series B Manufactured By Nuaire Inc 2100 Fernbrook Lane Plymouth MN 55447...

Страница 2: ...ction from particulate contaminants as prescribed in NSF ANSI 49 Please read this manual carefully to familiarize yourself with proper installation maintenance and operation of the cabinet Other refer...

Страница 3: ...rawings are appended in the back flap of this manual along with any modifications and or additions to procedures as outlined in this manual A copy of the original factory test report is also appended...

Страница 4: ...stallation Assessment Test 7 9 Cleanliness Classification Test for Pharmacy Application 7 10 Main Control Board Description and Replacement 7 11 Digital Airflow Sensor Description and Replacement Sect...

Страница 5: ...mental controls as required in the field of biological research or Hazardous Drug chemical containment The LFBSC when used with proper technique is an effective primary engineering control in obtainin...

Страница 6: ...ply while specific agents are used o which measures are to be taken in the case of an accident Repairs to the device must be carried out only by trained and authorized expert personnel Keep these oper...

Страница 7: ...OM0302 Rev 2 December 2020 Page 7 of 107...

Страница 8: ...0302 Rev 2 December 2020 Page 8 of 107 2 0 Models and Features The model NU 581 LabGard ES HD Class II Type A2 Laminar Flow Biosafety Cabinet is manufactured in four sizes 4 ft 1 2m 5 ft 1 5m 6 ft 1 8...

Страница 9: ...OM0302 Rev 2 December 2020 Page 9 of 107...

Страница 10: ...OM0302 Rev 2 December 2020 Page 10 of 107...

Страница 11: ...OM0302 Rev 2 December 2020 Page 11 of 107...

Страница 12: ...ed Shipments 4 1 1 Terms are factory unless stated otherwise Therefore it is important to check each shipment before acceptance 4 1 2 If there is visible damage the material can be accepted after the...

Страница 13: ...accessible for maintenance purposes Do not position the cabinet to prevent access to the power cord The power cord plug serves as the disconnect and should remain readily accessible If the outlet is...

Страница 14: ...lve stem to gain clearance for valve body rotation Add Loctite 242 furnished to the threads and rotate valve body until secure with the valve stem for handle on the left side 5 2 4 Gas Service Nuaire...

Страница 15: ...ries per cabinet size reference Electrical Environmental Requirements It is recommended that power to the cabinet whether hardwired or plug connected be on its own branch circuit protected with a circ...

Страница 16: ...installation assessment tests The testing methods and equipment required are specified on the factory inspection report included with this manual see insert in back cover NOTE IT IS RECOMMENDED THAT T...

Страница 17: ...ith Control Center And Armrest Removed 86 7 8 2207 92 7 8 2359 79 3 8 2016 54 7 8 1394 33 838 86 7 8 2207 92 7 8 2359 79 3 8 2016 66 7 8 1699 33 838 86 7 8 2207 92 7 8 2359 79 3 8 2016 78 7 8 2003 33...

Страница 18: ...y compensates for filter loading voltage variances and other environmental effects A second digital dual thermistor airflow sensor in the exhaust airstream monitors for inflow velocity Both downflow a...

Страница 19: ...icon may also be accessed for additional user menus 6 1 3 Run Mode Any time the blower icon is selected the RUN MODE screen will appear The Run Mode screen will display airflow setpoints and warm up...

Страница 20: ...nto a ring back cycle of once every 2 seconds Pressing the alarm silence icon will silence the audible alarm for 15 minutes then into a ring back cycle of once every 2 seconds Alarm Types The middle o...

Страница 21: ...essed will provide a list of menu items for various TouchLink functions 6 1 6 1 Setpoint Limits Setpoint Limits This menu screen will indicate the Biosafety Cabinet airflow control setpoints Access is...

Страница 22: ...ions A description of each timer function is provided below Pressing the time area on the menu will bring up an keypad for time entry for each function Timer Functions Purge Timer This timer controls...

Страница 23: ...ressed to turn on the outlet If timer is zero the outlet will stay on until turned OFF UV Light Timer This timer controls how long the UV light will remain on after the UV light icon has been pressed...

Страница 24: ...ll automatically turn on and off together on a programmed schedule Once into the auto timer menu select the desired day for the auto timer to function If multiple days are desired each day will be req...

Страница 25: ...6 1 6 3 Historical Performance When press a historical performance text the system displays downflow and inflow history Downflow History press downflow history the system displays the week log data i...

Страница 26: ...OM0302 Rev 2 December 2020 Page 26 of 107 Inflow History press downflow history the system displays the week log data in graphical form of Inflow versus days...

Страница 27: ...ovides the ability to alter display features and set date time Display Background colors of White Green Blue and Gray can be selected Touch display tone audible feedback can turn on off Display bright...

Страница 28: ...et the time and date displayed on the LCD screen Time displayed is real time and will not automatically adjust for day light saving time 6 1 6 6 Change User Password This menu item provides the abilit...

Страница 29: ...OM0302 Rev 2 December 2020 Page 29 of 107 Set New Password Retype new password...

Страница 30: ...e position The power window also incorporates a safety auto reverse feature to eliminate any pinch hazard Operating the power window is performed by pressing either the UP or DOWN window icon The trav...

Страница 31: ...e evaluated by appropriate safety personnel 6 2 2 Minimize Penetration of Air Curtain The minimum number of items necessary should be placed into the cabinet to prevent overloading but the work should...

Страница 32: ...his precaution is merely an extension of good aseptic technique as practiced on open bench tops The good laboratory practices designed to minimize creation and or release of aerosols to the environmen...

Страница 33: ...in some installations an additional advantage is obtained from purification filtration of the room air circulated through the equipment Because of this characteristic contributing to the quality of th...

Страница 34: ...e performed prior to removing the needle from the stopper of the vial e A non splash collection vessel should be available in the Biosafety cabinet to discard excess drug solutions 6 3 6 Terminal Purg...

Страница 35: ...unctionality and general good housekeeping The information provided below is intended to aid the development of facility Standard Operating Procedures SOP s for cleaning the equipment It is strongly r...

Страница 36: ...perforated metal diffuser screen from the underside of the supply HEPA filter and place on the cabinet work tray NOTE Depending on the model the diffuser screen is secured to the cabinet by 8 32 scre...

Страница 37: ...ust be performed only by specially trained and authorized service personnel in accordance with applicable national safety regulations The formaldehyde is vaporized within the tightly sealed sample cha...

Страница 38: ...is started by accessing the CABINET DECONTAMINATION on the calibration service menu AUTO DECON H2O2 CD The Auto Decon process requires the use of Front and Top Seal Plates Seal plates for BSC s are so...

Страница 39: ...ic via the upper and lower fasteners Refer to BCD 12281 At this point the sliding window assembly can be removed 4 Remove exhaust sensor shrouds 5 Remove the diffuser screen some methods of manual dec...

Страница 40: ...be wiped clean with a suitable tissue and water after each use 3 Gowns A protective garment must be worn The garment should be made of lint free low permeability fabric and must have a closed front l...

Страница 41: ...during the entire decon cycle To validate two decon processes and associated cabinet set up procedure Nuaire utilized a Bioquell Clarus II H O and a Steris X10 The following process was performed by...

Страница 42: ...OM0302 Rev 2 December 2020 Page 42 of 107...

Страница 43: ...Edges of the front window sealed with a low residue tape 6 Tubing connected to the inlet and outlet of X10 7 Insertion of Hydrogen Peroxide H2O2 sterilant cup and desiccant cartridge to X10 8 Current...

Страница 44: ...OM0302 Rev 2 December 2020 Page 44 of 107 Auto Decon Set Up Select AUTO DECON SET UP to enter the specific Decon cycle parameters Select DECON FULL CYCLE TIME...

Страница 45: ...wer run time at each interval as required by the automated decon process used Decon blower duty cycle allows the cabinet blower to run at a lower speed when used during the decontamination process The...

Страница 46: ...o Decon Run To initiate the auto decon run cycle select and enter AUTO DECON RUN To prevent unauthorized entry into the auto decon cycle the password must be entered The password would be the same as...

Страница 47: ...e auto decon H2O2 CD cycle Once the cycle has started it is desirable to let the cycle run until finished The display will indicate what portion of the cycle it s in and the time remaining It is possi...

Страница 48: ...ident a puncture do not need replacement until the efflux velocity cannot be maintained or the access inflow velocity cannot be maintained at 100 LFPM 51 m s min This may permit the average downflow v...

Страница 49: ...a Rotate both of the prefilter release clamps 90 cleaner and place wipe in prefilter Install prefilter seal over the used prefilter 6b Pull up and out prefilter of its position and lay on its Side to...

Страница 50: ...damaged i e torn replace it Nuaire part number A 3071 25 Proceed to install HEPA prefilter making sure filter is seated level onto the gasket NOTE Only use same type of HEPA prefilter to assure proper...

Страница 51: ...s handles to remove the panel NOTE Screws are used in lieu of acorn nuts and lock washers The screws have O rings and should be replaced if damaged or badly deformed The interior of the cabinet is now...

Страница 52: ...ons to the motor remove the HEPEX pressure plenum and unbolt the motor blower assembly from the roof of the cabinet 4 places Always inspect the rubber isolation motor mounts and replace those that are...

Страница 53: ...e of fall if a window counterbalance experiences a fault is also required to assure proper window function see Sliding Window Retention Verification in the Inspection Report 7 5 Airflow Control System...

Страница 54: ...pe The cabinet type can be verified in the control system and is factory set and shouldn t require alteration The cabinet type default information controls unit of measure setpoints and limits based o...

Страница 55: ...ress SET MOTOR TYPE to verify correct setting Upon a MASTER RESET the motor type is defaulted to DC ECM Single The NU 581 Series 1 115Vac requires the motor type selected to be DC ECM Single Always ve...

Страница 56: ...setpoint establishes the airflow values that are to be maintained The high low limits establish the alarm boundaries from the nominal setpoint The default values have been established based upon the p...

Страница 57: ...while the choke adjusts or balances the exhaust airflow as well as makes up for filter resistance tolerances Since it has been Nuaire s experience that the filters may not load evenly both adjustment...

Страница 58: ...one minute or until downflow readings are steady Step 3 Place a velometer in the cabinet workzone on the horizontal plane 4 inches 102mm above the bottom edge of the window Spot check several points...

Страница 59: ...S RELATED TO THE AIRFLOW MONITOR SENSOR CALIBRATIONS Step 1 Access sensor setup menu Step 2 Now measure the inflow velocity using the recommended procedure found in Table 7 0 If necessary adjust the e...

Страница 60: ...desired a spot check in the downflow velocity may be performed if felt necessary Once entire cabinet has been balanced tighten liquid tight fasteners around choke adjustment shaft 7 5 3 3 Alarm Verif...

Страница 61: ...9 19 620 498 19 380 492 Number of Readings Average Velocity ft min m s d Acceptance Criteria 1 Average downflow velocity 55 to 65 fpm 28 to 33 m s 2 Individual readings must be within 20 or 16fpm 0 08...

Страница 62: ...980 1168 50 178 1274 54 375 1381 600 4 102 8 158 207 12 316 313 16 474 481 20 632 524 24 790 630 28 948 735 33 106 841 37 264 946 41 422 1052 45 580 1158 49 738 1263 53 896 1369 58 054 1475 62 212 158...

Страница 63: ...of hazardous drug residue or appropriate precautions are taken per facility Standard Operating Procedures SOP S NOTE Cabinet should be on and running throughout the filter replacement process Hazardou...

Страница 64: ...th duct tape or other suitable means to prevent contaminated air from migrating into the exhaust efflux All exhaust blowers fans should be turned off during the check The exhaust sensor shroud can als...

Страница 65: ...sed from one end of the cabinet to the other 1 inch 25mm just inside the view screen at a height 6 inches 152mm above the top of the access opening 3 Pass a smoke source along the edges of the entire...

Страница 66: ...control test points using a voltmeter Step 2 Using the primary or secondary inflow test method lower the speed control voltage to reduce the inflow by 20 from the certified testing value Step 3 Verify...

Страница 67: ...ic compounding manipulations At Rest Particle Count Test Take 5 test points in 1 minute intervals on a grid in a horizontal plane as measured approximately 6 inches 152mm above the worksurface The gri...

Страница 68: ...1 4 INCH 5 X 20MM 5 X 20MM 5 X 20MM NU 581 400 15 AMPS 3 AMPS 2 2 AMPS 1 AMP NU 581 500 15 AMPS 3 AMPS 2 2 AMPS 1 AMP NU 581 600 15 AMPS 3 AMPS 2 2 AMPS 1 AMP 7 10 3 Main Control Board Replacement No...

Страница 69: ...an error in operation Cabinet type motor type and calibration data will not be affected with this reset Master Reset Resets all calibration cabinet type motor type sensor data and options settings ba...

Страница 70: ...2 Rev 2 December 2020 Page 70 of 107 Select desired function from menu Perform either reset function as selected below Once factory reset is complete return to Calibration Service menu to enter any op...

Страница 71: ...main menu At this point the cabinet MUST be turned off to complete the process Either unplug the cabinet or use the power switch within the control center to turn off the cabinet Turn the cabinet back...

Страница 72: ...any maintenance action The airflow sensors are removed by turning the locking ring counterclockwise and gently pulling the sensor away from the connector To reattach the airflow sensor turn sensor in...

Страница 73: ...n main control bd Display indicates Remote Override Active Indicates that the remote override is activated preventing the usage of the cabinet Power Loss Alert Indicates a power interruption has occur...

Страница 74: ...adjust display brightness screen Run Time Failure Indicates a temporary microprocessor issue Clean alarm a see it reoccurs check line voltage to control board replace control board Recertification Due...

Страница 75: ...ing the Calibration Service menu item a password screen will appear The default password is 9876 Once the service password is entered the Calibration Service menu will appear To exit either press the...

Страница 76: ...mode This means with no controls or alarms activated When the manual control is on the downflow and inflow displays will indicate nominal setpoints Airflow adjustments can be made in the manual mode...

Страница 77: ...off the blower may be turned on and off without restriction Night Setback setup The night setback setup menu item provides an access to night setback option and night setback Calibration Night setback...

Страница 78: ...setback calibration The night setback calibration is calibrated as a percentage of the nominal setpoint and should be adjusted during the certification process to the desired level The default for nig...

Страница 79: ...ice The service setup menu allows a QUALIFIED TECHNICIAN to configure calibrate and obtain functional service data Each parameter submenu will be described as well as the display will indicate present...

Страница 80: ...0302 Rev 2 December 2020 Page 80 of 107 UV Light Run Time UV light run time value Reset if replacing the UV lamp Blower Motor Run Time Blower Motor Run time or enter old value if replacing control boa...

Страница 81: ...fication time period Filter Maintenance This parameter allows the service technician to set the filter status data used to predict filter life availability Filter status is based on maximum RPM minus...

Страница 82: ...ry Setup Option Setup The option set up menu allows A QUALIFIED TECHNICIAN to configure several different optional parameters per the menu below Each parameter sub menu will be described as well as th...

Страница 83: ...he designated alarm silence time i e default time is 15 minutes When the alarm silence function is off airflow alarms cannot be silenced The alarm silence key will only appear for window high alarms t...

Страница 84: ...This parameter allows for the selection of time to determine how many continuous seconds of an alarm condition occurs before activating an audible and visual alarm The time is displayed in seconds wi...

Страница 85: ...terlock is on the window must be closed for the UV light to operate When the window interlock is off the UV light can be turned on regardless of the window position NOTE In addition to the TOUCHLINK s...

Страница 86: ...e to run when the window is closed o Fan Relay Interlock This parameter allows for the selection of the fan relay interlock operation When the fans relay interlock is on and the blower switch is pendi...

Страница 87: ...blower When the blower FL light interlock is on the LED light operation will be interlocked to the blower When the blower FL light interlock is off the LED light can be turned on at any time o Blower...

Страница 88: ...indow closure Once the sliding window is closed the LED light will be disabled and the airflow will be reduced to a percentage of the normal setpoint NOTE Blower window interlock must be turned off to...

Страница 89: ...be identical to the fan relay When the AUX relay function is off the AUX relay function provides delay On Off option o Accessory Outlet This parameter allows for the selection of the power connection...

Страница 90: ...display menu to indicate the power window up and down icons The power window option functions using a 24 Vdc motor and internal feedback encoder to determine window position The power window also inco...

Страница 91: ...nostics The General input and output diagnostics menu allows A QUALIFIED TECHNICIAN to exercise the control system s inputs and outputs Blower Sensor Diagnostic Blower sensor diagnostics reports the c...

Страница 92: ...n points or move into desired access opening height default window height is 10 inches 254mm Then the Auto Cal can be used to lock in the window position based on the activation points of the window h...

Страница 93: ...3mm past switch deactivation Bottom Middle Window Low Alarm Continues to travel up for 1 7 8 48mm past switch deactivation Used only with power window option Bottom Window Closed Stops when deactivat...

Страница 94: ...and then remove This action will cause the cabinet to go into a downflow alarm condition The exhaust airflow reading should increase during this test However the downflow reading should go down to zer...

Страница 95: ...and normally closed contact closure outputs which are activated whenever the bower is turned on However the AUX relay does have some conditional logic programmed The relay will activate whenever the...

Страница 96: ...located on the front panel The sliding window is interlocked to the ultraviolet light so when the sliding window is raised the ultraviolet light will turn off If operational time duration is known th...

Страница 97: ...Strep hermolyticus alpha type 5 500 Staphylococcus aureus 6 600 Staphylococcus albus 5 720 Micrococcus sphaeroides 15 400 Sarcina lutea 26 400 Pseudomonas fluorescens 7 040 Escherichia coli 7 040 Pro...

Страница 98: ...pital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply 11 5 Pollution Degree 2 0 Pollution degre...

Страница 99: ...ll Stainless Steel Worksurface Stainless Steel Window Faring Stainless Steel Window Glides HDPE Window Safety Glass Window Frame Stainless Steel Front Service Panel Painted Steel Front Decorative Pane...

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