Contents
v
5.2
Operating the Motorized XY Stage................................................................... 98
6 Adjusting
the
Diopter...............................................................................................99
7
Focusing and Centering the Condenser ...............................................................100
8
Adjusting the Aperture Diaphragm ........................................................................101
9
Using the Condenser ............................................................................................103
9.1
Compatibility of Condensers with the Magnification of Objectives ................ 103
9.2
Using the NI-CUD-E Motorized Universal Condenser (Dry) .......................... 104
9.3
Using the NI-CUD Universal Condenser (Dry)............................................... 105
9.4
Using the D-CUO DIC Condenser (Oil) ......................................................... 107
10 Adjusting
the
Field
Diaphragm ..............................................................................108
11 Switching
the
Objective.........................................................................................109
11.1 Motorized
Nosepiece Operation..................................................................... 109
11.2 Intelligent/Manual
Nosepiece Operation .........................................................111
12 Switching the Optical Path of the Tube ................................................................. 112
12.1
Optical Path Switching of the Motorized Quadrocular Tilting Tube.................112
12.2
Switching the Optical Path in a Manually-Operated Tube ..............................113
13 Adjusting the Binocular Section ............................................................................ 114
13.1
Adjusting with the Binocular Section of the Tube............................................114
13.2
Using the Eyelevel Riser .................................................................................114
14 Oil/Water
Immersion.............................................................................................. 115
15 Dark-field
Microscopy............................................................................................ 117
16 Epi-fluorescence
Microscopy ................................................................................ 118
16.1
Epi-fluorescence Cube Turret Operation (Switching Excitation Methods)......118
16.2 Selecting
Filters.............................................................................................. 120
16.3
Protecting the Sample and Preventing It from Decoloration
(Using the Shutter) ......................................................................................... 122
16.4
Adjusting the Brightness of the Fluorescent Image (Using ND Filters
and the Aperture Diaphragm)......................................................................... 124
16.5
Restricting the Illumination to the Area of the Specimen being Viewed
(Centering and Adjusting the Field Diaphragm)............................................. 126
16.6
Changing the Waveform Characteristics of the Excitation Light (D-FB
Excitation Balancer) ........................................................................................ 127
16.7
Switching the Excitation Filter (Motorized Excitation Filter Wheel)................ 128
16.8
Switching the Barrier Filter (Motorized Barrier Filter Wheel) ......................... 129
16.9
Other Notes on Epi-fluorescence Microscopy................................................ 129
17 Differential Interference Contrast Microscopy .......................................................130
17.1
Tips for Differential Interference Contrast Microscopy................................... 130
17.2 Using
Optical Elements.................................................................................. 131
18 Phase Contrast Microscopy ..................................................................................132
18.1
Tips for Phase Contrast Microscopy .............................................................. 132
18.2 Using
Optical Elements.................................................................................. 133
19 Useful
Functions ...................................................................................................134
19.1 MODE............................................................................................................. 134
19.2 Interlocking Function ...................................................................................... 138
20 Using the Ergo Controller......................................................................................141
21 Using the Joystick Controller.................................................................................144
22 Capturing
Images..................................................................................................145
23 Operation on DS-L3 ..............................................................................................150
23.1
Setting Up the Microscope ............................................................................. 150
23.2 Controlling
the Microscope............................................................................. 166
Содержание ECLIPSE Ni-E
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Страница 31: ...Chapter 2 Microscopy Operations 17 Before Microscopy Operation Flowchart ...