Chapter 3
Individual Operations
92
Indiv
idua
l
Op
eratio
n
s
4
Focusing on the Sample (Vertical Stage Movement)
Focus adjustment is performed using the focus knobs on the Ni-E main body, on the ergo controller or on the joystick
controller. The following instructions assume the use of the focus knobs on the main body. For details on using the ergo
controller or joystick controller, see “20 Using the Ergo Controller” and “21 Using the Joystick Controller”.
“Resetting the Z-axis coordinate”, “Refocusing,” and other useful functions are available for focus adjustment.
4.1
Proper Focusing Procedure
Notes on controlling the focus knobs
When operating the focus knobs on the main body, avoid the
following action, which can cause equipment malfunctions.
Rotating the right and left focus knobs in opposite directions.
Do not turn the focus knob!
Using an objective of high magnification may cause the
specimen to be pushed against the objective, damaging the
objective. Follow the procedure below to focus on the specimen
to avoid breaking the cover glass or damaging the objective.
(1) Bring the 10x objective into the optical path, and turn the
coarse focus knob to raise the stage to the upper limit.
(2) Direct 100% of the light to the binocular section.
(3) Adjust the brightness of dia-illumination.
(4) Look into the eyepiece and turn the coarse focus knob to
lower the stage and focus on the specimen.
(5) Turn the fine focus knob to further adjust the focus.
Focusing by the focus knob
P A
_ _
2 x
_ _ _ _
Z :
_ _
1 2 3 4 . 5 6 7 u m
U V - 2 B
'
C
_
Z M : 1
'
0 x
_
N D :
_
0 . 0 %
The value increases when the stage is raised,
and decreases when it is lowered.
Tips for focusing
When moving the stage with the coarse focus knob, move your eyes away from the eyepiece and operate the
microscope while looking at it from the side.
When working with the coarse focus knob while looking into the eyepiece, you should only turn the knob in the
direction for lowering the stage.
First use an objective of low magnification to adjust the focus, and then switch to an objective of higher
magnification.
As a 10x or 4x objective has a wider operating distance, the specimen never touches the tip of the objective even if
the stage is raised to the upper limit, provided that a slide and a cover glass of standard thickness are used.
(Standard thickness is 1.2 mm for the slide glass, and 0.17 mm for the cover glass.)
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