Nikon ECLIPSE Ni-E, Инструкция по эксплуатации

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Chapter 2
Microscopy Operations
38
Ep
i-fluo
resce
n
ce
Micro
sco
p
y
Preparation Focusing Microscopy
Epi-fluorescence
Microscopy Procedure
1 2 3 4 5 6 7 8 9 10 11 12 13 14 ■ ■ ■ ■ ■
Focus and Optical System Adjustment
8
Focus on the sample.
Focus on the sample by rotating the focus knob. (See
Chapter 3 “4.1 Proper Focusing Procedure”.)
Z-RESET
The Z-axis coordinate value can be reset to zero.
This is useful when you wish to use the current
position as the reference position in adjusting the
focus. (See Chapter 3 “4.3 Resetting the Z-axis
Coordinate”.)
Refocusing
This operation moves the Z-axis position to the
retracting position or returns it to the original position.
(See Chapter 3 “4.4 Refocusing”.)
Focusing using the focus knob
P F _ 4 0 x _ _ _ _ Z : _ _ _ _ _ 7 . 1 2 3 u m
U V - 2 B
'
O _ _ _ _ N D _ 1
'
O _ _ E P I : O
Z-axis coordinate
Note on controlling the focus knobs
Avoid the following action, which can cause equipment
malfunction.
•
Rotating the right and left focus knobs in opposite
directions.
Do not turn the focus knobs in opposite direction!
9
Center the field diaphragm of the epi-fluorescence attachment.
(1) Use the field diaphragm lever on the epi-fluorescence
attachment to close the field diaphragm, and then move
the field diaphragm image to the center of the field of
view by turning the field diaphragm centering screws
using a hex driver (2 mm across flats).
(2) Open the field diaphragm to fit the field of view, and
once again, turn the centering screw to move the field
diaphragm image to the center of the field of view.
Changing magnification
While it is not absolutely essential to center the field
diaphragm each time you change the objective, you
should still perform a quick check and make
adjustments as necessary.
F. STO
P
A. ST
OP
EX. ADJ
Centering the field diaphragm
Field diaphragm
image
Eyepiece
field ring
Field diaphragm
image
Eyepiece
field ring
Field diaphragm
lever