Nikon Eclipse 50i POL, Инструкция по эксплуатации

Страница: 49 / 73

Chapter 3 Individual Operations
3.16 Polarization Microscopy
47
3.16.3
Azimuth adjustment of the polarizer and analyzer
For the diascopic observation
(1) Push in the analyzer setting knob to move the
analyzer out of the optical path.
(2) Focus on the specimen.
(3) Pull out the analyzer setting knob to move the
analyzer into the optical path.
(4) Turn the analyzer rotation dial and align at
the “0” position on the scale.
(5) Move the dia polarizer into the optical path.
(6) Move the specimen out of the optical path.
(7) Move the Bertrand lens into the optical path.
The pupil of the objective will then be visible
through the eyepiece. Turn the dia polarizer
and adjust so that the dark cross image
appears in the pupil as shown in the figure.
This is so-called the crossed Nicols position,
where the orientations of the polarizer and
analyzer coincide with those of the orientation
plate on the top of the microscope base (the
polarizer, P, is in the X direction and the
analyzer, A, is in the Y direction).
0 10
OUT IN
0.
120
110
100
130
30
20
10
40
80
70
60
50
90
170
160
150
140
0
A
It should be noted that the X direction is explained as that of the analyzer and Y
direction as that of the polarizer in some commercially available technical
manuals and reference books.
(1) Push in the analyzer setting knob to move the analyzer out of the optical path.
(2) Place a dummy specimen. It must have high reflectance with optical isotropy, for
example a mirror. And then, focus on the specimen.
(3) Pull out the analyzer setting knob to move the analyzer into the optical path.
(4) Turn the analyzer rotation dial and align at the “0” position on the scale.
(5) Push in the polarizer slider on the epi illuminator to place the epi polarizer into the
optical path.
(6) Move the Bertrand lens into the optical path. Turn the polarizer rotating ring on the
epi polarizer and adjust so that the dark cross image appears in the pupil as shown in
the figure.
Dia polarizer
Orientation
plate
Analyzer
scale: 0
Dark cross