Nikon Eclipse 50i POL, Инструкция по эксплуатации

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Chapter 2 Microscopy
2.2 Orthoscopic Observation
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2.2
Orthoscopic Observation
This section describes the orthoscopic observation procedure. This is the characteristic
observation method of polarizing microscopes. In this method, the specimen is observed with
the polarizer and the analyzer placed in the optical path.
The shape of the specimen in the direction of the optical axis and its optical properties in the
direction of the thickness can be observed. The vibration direction of the light and the property
of the refraction can be measured with observations of light extinction or interference colors of
specimens and rotations of the stage.
1
Fosus on the specimen with the diascopic bright-field
microscopy. (Refer to “2.1 Diascopic Bright-field
Microscopy.”)
2
Pull the analyzer slider of the
intermediate tube to move the
analyzer into the optical path.
Set the scale for the analyzer to the “0” position.
And, remove the Bertrand lens from the optical path.
3
Set the polarizer to the bottom of
the condenser.
4
Adjust the orientation of the
polarizer and the analyzer.
⇒ P.47
After the adjustment, set the specimen again and focus
on it.
5
Perform the orthoscopic microscopy.
•
You can measure the retardation in various range with the P-CL 1/4 λ & tint plate.
（⇒
P.49
）
•
The condenser aperture diaphragm and the field diaphragm must be adjusted in the same
ways for the bright-field microscopy.
•
The top lens of the P swing-out condenser is used depending on the magnification of the
objective.
Objective Top lens position of the swing-out condenser
10X or higher IN
4X or lower OUT
Move the analyzer
into the optical path.
Set the polarizer.