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Chapter 3  Individual Operations 

 

3.13  Centering the Objective 

42 

3.13 

Centering the Objective 

 

 

 

To perform the polarization microscopy, the center of the objective optical path must be aligned 
to the rotation center of the circular graduated stage. This product comes with the centering 
nosepiece. You can perform the centering adjustment for each objective. 

Required tools:

  two centering tools (provided with the nosepiece) 

 

 

(1) 

Before centering the objecives, focus on a 
specimen using the 10X objective.

 

(2) 

Bring an appropriate target such as granules 
that can be easily used as a marker in the 
specimen to the center of the crosshairs of the 
eyepiece.

 

(3) 

Insert the centering tools into the centering 
screws on the nosepiece.

 

(4) 

Rotate the stage about 180°. Move the 
objective using the centering tools so that the 
center of the crosshairs moves by one-half the 
amount of movement of the target.

 

(5) 

Move the specimen and bring the target to the 
center of the crosshairs.

 

(6) 

Repeat this procedure several times. Carry out 
this centering procedure for each objective.

 

 

 

 

 

 

 

 

 

 

 

Target

180° 

rotation

 

Middle point 
of the 
movement

 

Special tools are provided with 
the nosepiece. Insert the special 
tools into the centering screw 
holes on both sides to center the 

objective.

 

Содержание Eclipse 50i POL

Страница 1: ...Polarizing Microscope ECLIPSE 50i POL Instructions M362E 05 8 NF 1 ...

Страница 2: ......

Страница 3: ...this system the super high pressure mercury lamp power supply high intensity light source and such WARNING and CAUTION Symbols Although Nikon products are designed to provide the utmost safety during use incorrect usage or failure to follow the safety instructions provided may cause personal injury or property damage Damage or injury that may occur due to mishandling is unwarranted To ensure corre...

Страница 4: ...he danger of biohazard To avoid biohazard contamination do not touch the contaminated portion with your bare hands Decontaminate the contaminated portion according to the standard procedure of your facility Caution for heat This marking on the lamphouse of the Eclipse 50i POL calls your attention on the following The lamp and its surroundings including the lamphouse become very hot during and imme...

Страница 5: ... ground terminal protective earth terminal Refer to Chapter 8 for the specified power cord To prevent electrical shock always turn off the main power switch press is to the O position of the product before attaching or detaching the power cord 5 Cautions about heat from the light source The lamp and its surroundings including the lamphouse become very hot during and immediately after lighting Do n...

Страница 6: ...ions Specified lamp 6V 30W PHILIPS 5761 4 Prevent contact with moisture Never allow water to come into contact with the product And do not use the product in circumstances where the product is splashed with water Water splashed onto any component of this product may cause short circuits resulting in malfunction or abnormal over heat If the product is subject to contact with water turn off the powe...

Страница 7: ...0 RH max no condensation 8 Remove any covers from the product before switching on Do not use the product while covered with a piece of cloth or so on as this will result in abnormal heat and fire hazards 9 Cautions at to concerning long sustained observations To relieve fatigue resulting from long observation sessions limit continuous observations to one hour Take at least 10 to 15 minute breaks b...

Страница 8: ... following factors when selecting an installation location Select a vibration free location Install the product on a level surface Install the product at least 10 cm away from walls Choose a location less exposed to hazards in the event of collisions earthquakes or other potential disasters To keep the product from falling use strong wire or other means if necessary to secure it to the working des...

Страница 9: ...e Doing so may damage the product Turning the coarse focus knob past its farthest point will damage the product Never use excessive force when turning the knob 7 Protect the ports from dust and extraneous light when the trinocular eyepiece tube is attached To keep out extraneous light and dust always attach the supplied cap to any port not currently in use ...

Страница 10: ...intuple centering nosepiece Nosepiece or centering nosepiece P swing out condenser P swing out condenser PT polarizer Dia polarizer LV UEPI universal epi illuminator LV UEPI or epi illuminator YM PO polarizer Epi polarizer LV LH50PC halogen precentered lamphouse Lamphouse Power supply device for the lamp Lamp power supply P ANH attachable mechanical stage Attachable mechanical stage Y TV TV vertic...

Страница 11: ... 3 Individual Operations 17 3 1 Power ON OFF 29 3 1 1 Power Supply for the Microscope 29 3 1 2 Power Supply for the Epi Illuminator 29 3 2 Brightness Adjustment 30 3 2 1 Adjusting the Brightness Control Knob 30 3 2 2 Adjustment Using the Preset Switch 31 3 2 3 Adjustment Using the ND Filter IN OUT Lever 31 3 2 4 Adjustment Using the Brightness Control Knob on the Power Supply for the Illuminator 3...

Страница 12: ...14 Oil Immersion Operation 43 3 15 Water Immersion Operation 44 3 16 Polarization Microscopy 45 3 16 1 Operation of the Polarizers 45 3 16 2 Operation of the Analyzer 46 3 16 3 Azimuth adjustment of the polarizer and analyzer 47 3 16 4 Bertrand Lens Operation 48 3 16 5 P CL 1 4λ Tint Plate 49 3 17 Episcopic Microscopy 51 3 17 1 Switching the Episcopic Illumination 51 3 17 2 Field Diaphragm in the ...

Страница 13: ... 6 2 Mechanical System 67 6 3 Electrical System 67 7 Care and Maintenance 30 7 1 Lens Cleaning 68 7 2 Cleaning the Product 68 7 3 Disinfecting the Product 68 7 4 Storage 69 7 5 Periodical Inspection Fee Charged 69 8 Specifications 32 8 1 Specifications 70 ...

Страница 14: ...piece Objective Vernier Stage rotation clamp screw Stage clamp screw Condenser P swing out condenser Dia polarizer bottom of the condenser Orientation plate Trinocular eyepiece tube Polarizing intermediate tube C mount adapter option TV vertical tube adapter option Microscope main body Condenser focus knob Coarse focus torque adjustment knob Coarse focus knob Fine focus knob Power switch Binocular...

Страница 15: ...s both sides Diopter adjustment i Bertrand lens turret Bertrand lens focus ring Specimen holders 2 Circular graduated stage Condenser aperture scale Condenser aperture diaphragm ring Condenser centering screws Field lens part Microscope main body Condenser focus knob Coarse focus stopper ring Coarse focus knob Fine focus knob ND filter IN OUT levers Brightness control knob Preset brightness contro...

Страница 16: ...wer supply for the epi illuminator is also required Lamphouse 3 Filter slider Dummy slider Epi illuminator LV UEPI Aperture diaphragm open close lever Field diaphragm centering screws both sides Field diaphragm open close lever Polarizer slider or dummy slider Illumination selection lever bright field dark field ...

Страница 17: ...er 1 Part Names 1 3 Rear Side 15 1 3 Rear Side 1 3 1 Rear Side of the Microscope 1 3 2 When the Lamphouse Cover Is Opened Halogen lamp Tool Handle Lamphouse AC inlet Input voltage indication Lamphouse cover ...

Страница 18: ...ff the episcopic illuminator And then pull the illumination selection lever to set the dark field position for it P 51 3 Rotate the brightness control knob to adjust the brightness Or push the preset switch to apply the voltage that wil make good color reproducibility P 30 4 Push in the analyzer slider on the polarizing intermadiate tube to remove the analyzer from the optical path P 46 Remove the...

Страница 19: ... the field diaphragm and the condenser aperture diaphragm 10 Set the optical path to make 100 light go into the binocular part when using the trinocular eyepiece tube P 33 Remove the Bertrand lens from the optical path Set the optical path to make 100 light go into the binocular part Set the 10X objective Set the specimen with the specimen holders Raise the condenser with the condenser focus knob ...

Страница 20: ...P 37 13 Center the objective P 42 14 Focus and center the condenser P 38 Move the condenser with the condenser focus knob Center the condenser with the condenser centering screws Focus on the specimen using the coarse focus knob and the fine focus knob Special tools are provided with the nosepiece Insert the special tools into the centering screw holes on both sides to center the objective ...

Страница 21: ...ser aperture diaphragm should be 70 to 80 of the numerical aperture of the objective P 39 P 41 Focus on the specimen again using the fine focus knob or the coarse focus knob P 34 To use an oil immersion type objective or a water immersion type objective perform the oil immersion or water immersion 16 Turn off the power after completing the observation Condenser aperture diaphragm knob Switch to an...

Страница 22: ...opy Refer to 2 1 Diascopic Bright field Microscopy 2 Pull the analyzer slider of the intermediate tube to move the analyzer into the optical path Set the scale for the analyzer to the 0 position And remove the Bertrand lens from the optical path 3 Set the polarizer to the bottom of the condenser 4 Adjust the orientation of the polarizer and the analyzer P 47 After the adjustment set the specimen a...

Страница 23: ...imen 1 Perform the orthoscopic observation Refer to 2 2 Orthoscopic observation 2 Rotate the Bertrand lens turret to the B position to move the Bertrand lens into the optical path 3 Focus and center the Bertrand lens P 48 4 Perform the conoscopic microscopy The P CL 1 4 λ tint plate is not used in this microscopy Move it to the vacant position Select an objective having a large numerical aperture ...

Страница 24: ...r of the microscope is supplied the diascopic illumination will be lit If you wish to use the epi illumination and the diascopic illumination simultaneously see 2 1 Diascopic Bright field Microscopy to adjust the diascopic illumination 2 Set the optical path to make 100 light go into the binocular eyepiece when using the trinocular eyepiece tube 3 Push in the analyzer slider on the polarizing inte...

Страница 25: ...he bright field position for it 6 Turn on the lamp power supply for the epi illumination Rotate the brightness control knob to adjust the brightness 7 Move the 10X objective into the optical path 8 Set the specimen in place with the cover glass facing up Set the specimen with the specimen holders Adjust the brightness Turn on the power supply Push in the illumination selection lever BF Set the 10X...

Страница 26: ...ath 11 Adjust the brightness with the ND filters 12 Focus on the specimen Fully loosen the coarse focus stopper ring P 34 Focus on the specimen using the coarse focus knob and the fine focus knob Move the open close lever to upper position to fully open the field diaphragm and the aperture diaphragm Move the NCB 11 filter into the optical path And adjust the brightness with ND filters ...

Страница 27: ...r into the place on the side of the illuminator and place the analyzer on the intermediate tube into the optical path The polarizer azimuth should be adjusted with the ring on the polarizer slider For the adjustment method see P 45 To perform the conoscopic microscopy move the Bertrand lens into the optical path See 2 3 Conoscopic Observation 15 To return to the bright field microscopy Turn off th...

Страница 28: ...that moving the stage left right moves the image on the monitor in the opposite direction After making the appropriate adjustments tighten the screws firmly Adjust the directions A and B 3 Make camera settings For detailed information refer to the operating manual provided with the camera When using the DS L1 you must choose and enter at least the following information Folder for data storage Name...

Страница 29: ... the focus Adjust the image brightness using the camera exposure compensation function Check the image using the Freeze button When the image is acceptable press the CAPT button to save the image The operating procedure differs if DF FL scene mode is selected For details see the manual for the camera ...

Страница 30: ...ondenser focus knob condenser centering screws 3 9 Adjusting the aperture diaphragm Condenser aperture diaphragm objective 3 10 Selecting a condenser Condenser 3 11 Adjusting the field diaphragm Field diaphragm control 3 12 Setting a filter on the field lens NCB filter ND 16 filter GIF filter 3 13 Centering the objective Centering nosepiece objective 3 14 Oil immersion operation Oil immersion type...

Страница 31: ...croscope is on the power indicator on the right side of the microscope is lit 3 1 2 Power Supply for the Epi Illuminator To turn on the power supply press the power switch to the I position The lamp will be lit To turn off the power supply press the power switch to the O position The lamp will be turned off When the power to the power supply is on the power indicator on the front of the power supp...

Страница 32: ...iscopic images Power switch for the microscope 3 2 6 Monitor image Camera adjustment Application software for camera control display mode exposure mode exposure compensation camera gain adjustment and so on 3 2 7 3 2 1 Adjusting the Brightness Control Knob With the preset switch in the out position rotate the brightness control knob The brightness control knob is disabled if the preset switch is d...

Страница 33: ...t switch to the depressed position enables the brightness level set with the preset brightness volume control 3 2 3 Adjustment Using the ND Filter IN OUT Lever Pushing in the upper lever moves the ND filter light intensity adjustment filter into the optical path and reduces brightness The color balance of the image remains unaffected 3 2 4 Adjustment Using the Brightness Control Knob on the Power ...

Страница 34: ...16 1 Out Out 1 4 In Out 1 16 Out In 1 64 In In Out out of the optical path In in the optical path 3 2 6 Transmitted Image in the Episcopic Microscopy To perform the episcopic microscopy with the epi illuminator turn off the power switch of the microscope to extinct the transmitted image in normal times 3 2 7 Camera Adjustment Adjusting the Brightness of the Image on the Monitor When observing imag...

Страница 35: ...nted on the vertical tube section of the trinocular eyepiece tube To mount the adapter remove the cap from the vertical tube section of the trinocular eyepiece tube insert the vertical tube adapter and fix it with three screws on the vertical tube section with the attached tool 3 3 3 Disabling the Clicking of the Optical Path Selection Lever The trinocular eyepiece tube has a NO CLICK switch on th...

Страница 36: ...focus knob located on both sides of the microscope You can move the stage up down to focus on the specimen by rotating these knobs To lower the stage Turn the knob toward the front To raise the stage Turn the knob toward the back 3 4 3 Number of Knob Turns and Distance of Stage Travel Number of knob turns Distance of stage travel vertical direction Coarse focus knob 1 turn Approx 13 8 mm Fine focu...

Страница 37: ... move the stage any higher Movement of the stage by the fine focus knob is not restricted If you must change specimens and move the stage up down repeatedly it will save your time for focusing that the coarse focus stopper ring is fixed at the focus point Example usage With the sample in focus turn the coarse focus stopper ring as far as it goes in the direction of the arrow labeled CLAMP When cha...

Страница 38: ...chanical stage is installed by inserting the two pins on the bottom into the two pinholes on the stage surface Tighten the clamp screw using the supplied hexagonal wrench To move the specimen position under observation rotate the knob on the stage You can adjust the position in the X direction and Y direction individually Travel range 35 x 25 mm The travel amount can be read to 0 1 mm with the two...

Страница 39: ...ve the right eyepiece with the right eye Turn the fine coarse focus adjustment knob to bring the specimen on the stage into focus 3 Observe the left eyepiece with the left eye Turn the diopter adjustment ring on the eyepiece to bring the specimen into focus not the fine coarse focus adjustment knob 3 7 Interpupillary Distance Adjustment Interpupillary adjustment improves the ease of binocular obse...

Страница 40: ...4 Turn the condenser centering screws so that the field diaphragm image is positioned in the center of the view field 5 Move the 40X objective into the optical path Turn the coarse fine focus knobs and focus on the specimen 6 Turn the condenser focus knob to form the field diaphragm image on the specimen surface 7 Turn the condenser centering screws so that the field diaphragm image is positioned ...

Страница 41: ...are reduced It is not possible to adjust one pair of characteristics without affecting the other Generally a satisfactory image with appropriate contrast can be obtained with an aperture setting that is 70 to 80 of the numerical aperture of the objective The numerical aperture is indicated on the barrel of each objective An indication of 40 0 65 means that the magnification is 40 and the numerical...

Страница 42: ...ve may not be achieved For example when an objective with an N A of 1 4 is used the maximum aperture of the P swing out condenser will be only about 65 of the objective s N A even when the condenser aperture diaphragm is fully open How to use the P swing out condenser The top lens of the P swing out condenser can be moved outside the optical path with the swing out knob During normal bright field ...

Страница 43: ... of the specimen Appropriate field diaphragm settings are particularly important for photomicrography and digital image capturing In general good results will be obtained by stopping down the field diaphragm to settings slightly wider than the area to be reproduced within the photo frame or monitor display For the field diaphragm of the epi illuminator refer to 3 16 Episcopic microscopy 3 12 Setti...

Страница 44: ... target such as granules that can be easily used as a marker in the specimen to the center of the crosshairs of the eyepiece 3 Insert the centering tools into the centering screws on the nosepiece 4 Rotate the stage about 180 Move the objective using the centering tools so that the center of the crosshairs moves by one half the amount of movement of the target 5 Move the specimen and bring the tar...

Страница 45: ...lace it with new oil Use as little oil as possible just enough to fill the space between the tip of the objective and the specimen or between the tip of the condenser and the specimen If too much oil is applied the excess oil will flow onto the stage or around the condenser Wipe off oil Any oil remaining on the oil immersion objective or adhering to the dry type objective will noticeably degrade i...

Страница 46: ...opy procedures are the same as for oil immersion type objectives Since water evaporates readily monitor the immersion water during observation Avoid using too much water since excess water will flow onto the stage and around the condenser promoting corrosion Wipe off water After use wipe off water from the tip of the objective and condenser then follow up by wiping with absolute alcohol If you obs...

Страница 47: ... for the episcopic microscopy To perform episcopic polarization microscopy attach the polarizer for the episcopic microscopy into the epi illuminator Insert the polarizer slider with the orientation index facing front side eyepiece side Pushing the polarizer slider in to the first clickstop position inserts the empty hole into the optical path Pushing it further in to the second clickstop position...

Страница 48: ...s scale displays the opposite way Be careful Slider Analyzer Pulled out IN Push in OUT Rotate the analyzer The analyzer slider has a rotating dial The orientation of the analyzer can be rotated with it To adjust the analyzer loosen the analyzer rotation clamp screw and rotate the rotating dial The angle of rotation can be read from 0 to 180 degrees in steps of 0 1 degrees with the two vernier scal...

Страница 49: ...microscope base the polarizer P is in the X direction and the analyzer A is in the Y direction 0 10 OUT IN 0 1 2 0 1 1 0 10 0 1 3 0 3 0 20 10 4 0 8 0 7 0 6 0 50 90 170 1 6 0 1 5 0 140 0 A It should be noted that the X direction is explained as that of the analyzer and Y direction as that of the polarizer in some commercially available technical manuals and reference books 1 Push in the analyzer se...

Страница 50: ...ive Note that you need not center the Bertrand lens each time if you have centered the objective already P 42 In this adjustment the aperture diaphragm image is used in the same manner as the condenser lens adjustment Do as follows 1 Refer to 2 1 Diascopic Bright Field Microscopy to focus on the specimen And then focus and center the condenser 2 Move the Bertrand lens into the optical path 3 Stop ...

Страница 51: ... path This plate is used for recognition of very weak birefringence and the determination of X and Z of the specimen You can measure retardation of light up to 1λ with the following steps P CS Senarmont compensator Remove the P CL 1 4λ tint plate from the slot of the intermediated tube and insert the P CS Senarmont compensator into the slot You can measure retardation of light up to 1λ with the fo...

Страница 52: ... Insert the P CQ quartz wedge into the slot of the intermediate tube and confirm that the interference color of the section of the specimen to be measured changes toward the lower order side If the interference color changes toward the higher order side rotate the stage another 90 3 Measurement Move the section of the specimen to be measured to the center of the crosshairs of the eyepiece Next sli...

Страница 53: ...ic illumination The dark field microscopy is not available 3 17 2 Field Diaphragm in the Epi Illuminator The field diaphragm restricts illumination to the area of the specimen that is being viewed Operating the field diaphragm open close lever changes the size of the field diaphragm For normal observation the size of the diaphragm should be such that it is just outside or inside the edge of the vi...

Страница 54: ...ragm image circumscribes the view field 3 17 3 Aperture Diaphragm in the Epi Illuminator Since the aperture diaphragm is for adjusting the numerical aperture of the illumination system this diaphragm is related to the resolution contrast and depth of focus of the optical image The aperture diaphragm open close lever will change the opening of the aperture diaphragm Remove one of the eyepieces and ...

Страница 55: ...You can use suitable filters by operating the filter sliders Filters Usage NCB11 color balancing filter For color balance adjustment and color photomicrography ND4 ND filter Brightness adjustment transmittance 25 ND16 ND filter Brightness adjustment transmittance 6 GIF green interference filter Contrast adjustment IF interference filter For interference Filter sliders ...

Страница 56: ...ess control knob to a midpoint setting and use the ND filters to make brightness adjustments Filter Place a commercially available color compensation filter on the filter pocket at the microscope base as necessary 3 18 2 Adjusting the Condenser Focus and center the condenser always Center the annular diaphragm for the phase contrast microscopy For normal operations set the diaphragm aperture to 70...

Страница 57: ...ghtly wider than the area shown on the monitor Eyepiece Cover the eyepiece with a piece of cloth 3 18 6 Anti vibration Measures If the exposure is less than 1 8 of a second reduce light intensity with ND filters to make exposures longer than 1 8 of a second If accurate color reproduction is not important you can use the brightness control knob to reduce light intensity ...

Страница 58: ...Polarizing intermediate tube with analyzer slider P CL 1 4λ tint plate P CS Senarmont compensator P CQ quartz wedge 12V 50W halogen lamp Lamphouse Quintuple centering nosepiece DIN slot Objectives Specimen holders x2 or Attachable mechanical stage Epi illuminator Lamp cable Power cord Lamp power supply Lamp cover Power cord 6V 30W halogen lamp 50i POL main unit Circular graduated stage P swing out...

Страница 59: ...ew must be located facing the front side 4 If necessary attach the specimen holders into the hole on the stage For information about the optional attachable mechanical stage see 3 5 2 Attachable Mechanical Stage Option 3 Attaching the condenser To perform the polarization microscopy the P swing out condenser must be attached 1 Turn the coarse focus knob until the elevating section is raised to the...

Страница 60: ... stage completely and hold each objective using both hands so that it does not fall during the removal 6 Attaching the epi illuminator for the episcopic microscopy The LV UEPI universal epi illuminator can be attached to this microscope 1 Loosen sufficiently the illuminator clamp screw on the side of the microscope arm 2 Mount the illuminator onto the microscope arm and fix it by tightening the il...

Страница 61: ...e top side of the lamphouse connector by using the hexagonal screwdriver 2 Mount the lamphouse to the connection port on the rear of the illuminator and press the lamphouse as far as it goes 3 Using the hexagonal screwdriver supplied with the microscope tighten the clamp screw on the top of the connection port of the lamphouse to secure it 4 Connect the cable of the lamphouse with the connector on...

Страница 62: ...ube 1 Completely loosen the eyepiece tube clamp screw on the intermediate tube with the provided tool 2 Fit the circular dovetail of the eyepiece tube into the circular dovetail groove of the intermediate tube When fitting insert the positioning pin on the eyepiece tube in the receiving groove on the intermediate tube 3 Secure the eyepiece tube by tightening the clamp screw Remove any looseness be...

Страница 63: ...itable adapter beforehand 1 Mount the TV vertical tube adapter or the TV vertical tube adapter 0 55X onto the vertical tube section of the trinocular eyepiece tube 2 Attach the suitable adapter for the camera onto the tip of the TV vertical tube adapter 3 Attach the camera head to the adapter tip 4 Attach the camera cable to the camera head Adjust the camera head position before using the camera 1...

Страница 64: ...nds Soiling will reduce the service life of the lamp Replacing the lamp for the diascopic illumination 1 Remove the lamphouse cover on the back of the microscope Remove the old lamp 2 Attach a new lamp Avoid touching the bare lamp bulb with your bare hands 3 Replace the cover Engage the cover hook in the slot on the rear of the unit in the direction indicated by the arrow in the figure Be sure to ...

Страница 65: ...placing the lamp Use the Nikon LV HL50W 12V 50W LONGLIFE halogen lamp or non Nikon 12V 50W SHORTLIFE halogen lamp model OSRAM HLX 64610 OSRAM HLX 64611 or PHILIPS 7027 for the lamp If you wish to buy these lamps please contact your nearest Nikon representative Do not touch the glass surface of the lamp with bare hands Fingerprints or grease on the bulb surface will reduce the illumination intensit...

Страница 66: ...s of a new lamp into the pin holes of the socket Press the lamp as far as it goes and then release the lamp clamp lever to secure the lamp Be careful not to touch the glass surface with bare hands When releasing the lamp clamp lever use care so that the lamp does not tilt 5 Close the lamphouse cover and secure it by tightening the clamp screw Be sure to attach the lamphouse cover Failure to do so ...

Страница 67: ...tted or uneven in brightness The P CL P CS or P CQ plate is not inserted correctly Move it to the correct position Dirt or dust exists on the lens or other optical element or on the container Clean off the dirt Use a clean container Dirt or dust is seen in the viewfield The field diaphragm image is not focused on the specimen surface Focus and center the condenser Dirt or dust exists on the lens o...

Страница 68: ...he condenser has not been centered Center the condenser The image is elongated The stage has been attached in out of perpendicular Install the stage correctly The lamp voltage is too low Increase the lamp voltage with the brightness control knob Or push the preset switch The image is tinged yellow No NCB filter is placed in the optical path Place it into the optical path No ND filter is placed in ...

Страница 69: ...tance When viewing through the binocular eyepiece the image does not resolve into a single image The diopter adjustment has not been performed Perform the diopter adjustment The interpupillary distance has not been adjusted Adjust the interpupillary distance The diopter adjustment has not been performed Perform the diopter adjustment Eye strain develops while viewing The brightness is not suitable...

Страница 70: ...face repeatedly Usually three or four times are sufficient to clean the lenses Never use petroleum benzine to clean the entrance lens of the eyepiece tube or prism surface of the eyepiece tube Absolute alcohol and petroleum benzine are highly flammable Be careful when handling it when around open flames when turning the power switch on off etc When using petroleum benzine or absolute alcohol alway...

Страница 71: ...he dust proof cover over this product to protect it from dust Before putting on the dust proof cover turn off the power switch of the microscope flip it to the O position and wait until the lamphouse gets cool sufficiently 7 5 Periodical Inspection Fee Charged Periodical inspections expenses charged of this product are recommended in order to maintain peak performance Contact your nearest Nikon re...

Страница 72: ...diascopic illumination optics Provided with a preset switch and a preset brightness control Provided with a brightness control knob Lamp ratings 6V 30W halogen lamp Philips 5761 Average service life of the lamp 100 hours Input ratings 100 120VAC 230VAC 10 50 60Hz 0 9A 0 5A Power cord When used in 100 120V areas UL listed detachable power cord set 3 conductor grounding Type SVT AWG 18 3 m long maxi...

Страница 73: ...is equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense This Class A digital apparatu...

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