69
Leica CM1860/CM1860 UV
Troubleshooting
8
Problem
Cause
Remedy
Thick/thin sections.
•
Temperature incorrect for the
tissue to be sectioned.
•
Select the correct temperature
and wait until the correct
temperature is reached.
• Knife profile being used
is inappropriate for this
specimen.
• Use knife with different profile
(c or d) or switch to disposable
blades if necessary.
•
Ice formation on back of knife.
•
Remove ice.
•
Handwheel speed not uniform
or turned at incorrect speed.
•
Adapt speed.
•
Knife not clamped tightly
enough.
•
Check clamping.
•
Specimen holder not clamped
tightly.
•
Check clamping.
•
Cryocompound applied to cold
specimen disc; specimen can
detach from the disc after
freezing.
•
Apply cryocompound on
lukewarm disc; mount
specimen in cryochamber and
freeze it.
•
Dull knife.
•
Use a different part of the
knife.
•
Incorrect clearance angle.
•
Set the correct clearance
angle.
•
Dried specimen.
•
Prepare new specimen.
Tissue adheres to the anti-roll
plate.
•
Anti-roll plate is too warm or
incorrectly positioned.
•
Cool down anti-roll plate or
reposition correctly.
•
Static charge.
•
Remove static charge.
•
Grease on the corner or edge
of anti-roll plate.
•
Remove grease using alcohol.
•
Rust on the knife.
•
Remove rust.
Flattened sections curl up when
anti-roll plate is folded up.
•
Static charge or air current.
•
Remove static charge.
•
Anti-roll plate too warm.
•
Cool down anti-roll plate.
Sections tear or separate.
•
Temperature too low for the
tissue being sectioned.
•
Select higher temperature and
wait.
•
Dull part, dirt, dust, frost or
rust on the knife.
•
Remove cause, clean.
•
Top edge of the anti-roll plate
damaged.
•
Replace anti-roll plate.
•
Hard particles in the tissue.
•
If the application permits, cut
deeper during sectioning.
•
Dirt on back of knife.
•
Clean.
•
Nick in the cutting edge of the
blade or knife.
•
Use a different part of the
blade/knife.
Inconsistent or inaccurate
specimen feed.
•
Microtome defective.
•
Call technical service.