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Rotors and accessories

35

Handling micro plates

Remove the appropriate plate holder from
the carrier body for loading and unloading
micro plates in the Highplate

®

Rotor

7500 6444.

Before loading, ensure that the rubber bottom is placed
in the cut-outs of the bottom of the plate holder. Deep-
well plates can also be inserted directly to the carrier
without plate holder.

Make sure the rotor is balanced!

The plate carriers are inserted as shown in the illustra-
tion below.

High

conic

®

- Rotor 7500 3057

In case longer tubes shall be centrifuged
precluding the complete locking of the rotor
cover, it is allowed to operate the rotor
without cover up to a maximum speed of
4000 rpm.

Above 4000 rpm the rotor is not
allowed to be operated without
cover in any case. Otherwise the
rotor could be destroyed.

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Содержание Sorvall Legend RT

Страница 1: ...SORVALL Legend T RT Instruction Manual QUIKset...

Страница 2: ...nnot provide protection against improper handling and thus against damage to persons and objects The manual comprises chapters on Safety regulations Instrument description Rotor program and accessorie...

Страница 3: ...located on the right hand side panel Pressed up ON I Pressed down OFF 0 only for instruments with refrigeration unit temperature Bucket selection rpm RCF switch key display Before switching on the cen...

Страница 4: ...a run open lid open lid possible only with the instrument switched on Quick run short term operation of the centrifuge as long as key remains pressed rpm RCF switch switching between rpm and RCF disp...

Страница 5: ...eration 36 Checking of aerosol tight bio containment 39 Operation 41 Switching on the centrifuge 41 Actuating the lid 41 Opening the lid 41 Closing the lid 41 Installing the rotor 42 Loading the rotor...

Страница 6: ...m 54 Turning the centrifuge off 55 Maintenance and care 57 Maintenance to be performed by the customer 57 Cleaning 57 Disinfection 58 Decontamination 60 Autoclaving 60 The KENDRO service offer 61 Warr...

Страница 7: ...s When damages at the power cord or at casing were noticed the centrifuge has to be set out of operation Proper use The centrifuge is designed to separate liquid suspended materials having different d...

Страница 8: ...nal accessories for the centrifuge The only exception are common glass or plastic centri fuge tubes if they are approved for the rotor speed and RCF values Never use the centrifuge unless the rotor is...

Страница 9: ...in this manual This symbol denotes potential hazards to persons This symbol denotes potential damage to the centrifuge or parts in its immediate surroundings General hints are marked with this symbol...

Страница 10: ...For your safety 6 Notes...

Страница 11: ...r RCFs more rapidly than competitive instruments and can process nomi nally three liters of sample in a single run There are various rotors available that can achieve high RCFs and accommodate a wide...

Страница 12: ...e to a stop You can start the centrifuge only if the lid is properly locked Automatic rotor identification Electronic imbalance detection as a function of rotor SMARTspinTM Do not tamper with the safe...

Страница 13: ...sy care continuous surface Control Microprocessor driven by QUIKset Main memory Recalls last run parameters Advanced features RCF programming quick run pretemp temperature control during standby Decel...

Страница 14: ...n of RCF switch the RCF value can then be entered Time selection adjustable in minutes from 1 min to 9 h 59 min or extended time mode from 1 min to 99 h hld mode continuous operation Run time display...

Страница 15: ...vates maximum acceleration up to the maximum permissible speed of rotor upon key release centrifuge stops with maximum deceleration power Diagnostic messages alternating display Rotor maximum speed or...

Страница 16: ...Description of the 12 Notes...

Страница 17: ...and secure it properly Handle the centrifuge carefully Proper location The centrifuge may only be used indoors Its location must meet the following criteria A safety zone of at least 30 cm 12 inches...

Страница 18: ...n connection Connect the centrifuge only to an grounded main power supply Make sure that the power cord is com patible with the safety regulations valid and that your main voltage and frequency corres...

Страница 19: ...urrent sales documentation For more information you can visit our web site at http www Kendro com Legend T air cooled During centrifugation heat is generated by air friction between the rapidly spinni...

Страница 20: ...croplates 7500 6449 Maximum permissible load g 4 x 800 4 x 750 4 x 500 Maximum speed nmax rpm 4150 4350 3750 4350 4150 4350 Maximum RCF value at nmax 3696 4083 2986 4020 3061 3364 Radius max cm 19 2 1...

Страница 21: ...carriers 20 x 10 15 ml 7500 6471 max tube dimension d 1 x l mm 61 x 180 14 x 120 17 x 140 Maximum permissible load g 4 x 325 4 x 400 25 x 16 4 x 520 20 x 26 Maximum speed nmax rpm 3750 4350 3750 4350...

Страница 22: ...ml 7500 2023 Multiple carriers 7 x 50 ml 7500 2024 max tube dimension d 1 x l mm 17 x 120 29 5 x 120 Maximum permissible load g 17 x 29 7 x 85 Maximum speed nmax rpm 3750 4350 3750 4350 Maximum RCF v...

Страница 23: ...500 6444 Maximum permissible load g 4 x 600 2 x 500 Maximum speed nmax rpm 5350 5250 Maximum RCF value at nmax 5824 5083 Radius max cm 18 2 16 5 Acceleration deceleration time s 145 70 85 70 110 55 85...

Страница 24: ...50 ml 7500 3057 Maximum permissible load g 6 x 140 6 x 130 Maximum speed nmax rpm 10 500 8500 Maximum RCF value at nmax 12 449 10 016 Radius max min cm 10 1 6 3 12 4 6 0 Tube angle 26 45 Acceleration...

Страница 25: ...500 6449 Maximum permissible load g 4 x 800 4 x 750 4 x 500 Maximum speed nmax rpm 4150 4600 3750 4600 4150 4600 Maximum RCF value at nmax 3696 4566 2986 4494 3061 3761 Radius max cm 19 2 19 0 15 9 Ac...

Страница 26: ...x 10 15 ml 7500 6471 max tube dimensions d 1 x l mm 61 x 180 14 x 120 17 x 140 Maximum permissible load g 4 x 325 4 x 400 25 x 16 4 x 520 20 x 26 Maximum speed nmax rpm 3750 4600 3750 4600 3750 4600 M...

Страница 27: ...0 2023 Multiple carriers 7 x 50 ml 7500 2024 max tube dimensions d 1 x l mm 17 x 120 29 5 x 120 Maximum permissible load g 17 x 29 7 x 85 Maximum speed nmax rpm 3750 4600 3750 4600 Maximum RCF value a...

Страница 28: ...permissible load g 4 x 600 2 x 500 Maximum speed nmax rpm 5350 5850 5250 5650 Maximum RCF value at nmax 5824 6963 5083 5888 Radius max cm 18 2 16 5 Acceleration deceleration time s 135 60 95 75 135 60...

Страница 29: ...3057 Maximum permissible load g 24 x 4 6 x 140 6 x 130 Maximum speed nmax rpm 15 000 12 000 8500 Maximum RCF value at nmax 21 885 16 260 10 016 Radius max min cm 8 7 5 9 10 1 6 3 12 4 6 0 Tube angle 4...

Страница 30: ...wn 7500 8137 12 x 14 15 ml with flange 18 3 x 125 21 5 48 brown 7500 6494 7 x 25 ml DIN 25 0 x 125 31 0 28 orange 7500 8138 7 x 45 50 ml flat and round bottom 29 5 x 125 31 0 28 blue 7500 6493 4 x 50...

Страница 31: ...14 15 ml with flange 18 3 x 117 25 0 36 brown 7500 6492 6 x 25 ml DIN 25 0 x 117 28 0 24 orange 7500 6457 4 x 25 50 ml universal container 25 5 x 117 32 0 16 blue green 7500 6459 4 x 45 50 ml flat and...

Страница 32: ...US urine 16 5 x 120 24 0 36 olive brown 7500 6456 9 x 14 15 ml with flange 18 3 x 117 25 0 36 brown 7500 6492 6 x 25 ml DIN 25 0 x 117 28 0 24 orange 7500 6457 4 x 25 50 ml universal container 25 5 x...

Страница 33: ...tor 7500 3057 max tube dimensions d 1 x length mm number per adapter number per rotor color order no 1 5 ml micro tube 11 x 58 4 24 nature 7600 2905 3 5 ml 11 x 103 4 24 nature 7500 3091 6 5 ml 13 x 1...

Страница 34: ...r per adapter number per rotor color order no 1 5 ml micro tubes 11 x 51 4 24 nature 7600 2905 3 5 ml 11 x 95 4 24 nature 7500 3091 6 5 ml 13 x 108 2 12 nature 7500 3092 12 ml 16 x 89 2 12 nature 7500...

Страница 35: ...rectangular buckets belonging to BIOshield Ro tors All positions have to be loaded with identical carrier buckets always The various swinging buckets are split up into weight categories These can be i...

Страница 36: ...maximum loading height is 60 mm with a base of 127 x 85 mm Tissue culture bucket 7500 6497 Large volume tissue culture tubes of 175 to 250 ml capacity available from various manufacturers can be used...

Страница 37: ...urning the cover locking knob Store the BIOshield Rotor with the cover removed after cleaning This will enable the rotor to dry thoroughly The rotor has to be retired upon reaching the end of its life...

Страница 38: ...ear 5 years 7 years average use 7 runs day 220 days year 14 years 20 years Always maintain the rotor in the recom mended manner The rotor and accessories must be clean and inspected regularly do not u...

Страница 39: ...lates can also be inserted directly to the carrier without plate holder Make sure the rotor is balanced The plate carriers are inserted as shown in the illustra tion below Highconic Rotor 7500 3057 In...

Страница 40: ...equisites for aerosol bio containment Before use the seals in the rotors and rotor covers as well as the aerosol tight caps have to be checked for abrasion or damage and slightly greased Replace damag...

Страница 41: ...he centrifuge e g in a safety work bench one has to care that during the installation into the centrifuge the rotor should be kept in a vertical position Closing the aerosol tight bio containment Afte...

Страница 42: ...d loosen the lid of the fixed angle rotor in order to achieve secure closing insert the hexagon through the hole in the screw cap Please mind the maximum permissible filling volume during centrifugati...

Страница 43: ...there is the possibility to check the aerosol tight buckets and fixed angle rotors according to the following procedure grease slightly all seals Fill the bucket or rotor with approx 50 ml carbon diox...

Страница 44: ...Rotors and accessories 40 Notes...

Страница 45: ...remaining run time and speed should both read 0 The display of the deceleration curve depends on the last set value The following figure gives an example of possible read ings A detailed description...

Страница 46: ...loosen the rotor seat using the socket wrench supplied 3 Place the rotor on top of the collet chuck so that the rotor is located precisely above the center 4 The rotor must glide freely down the coll...

Страница 47: ...er this safety system presupposes that the maximum permissible load of the rotor is not exceeded Please note the data about the maximum permissible loads and maximum speeds in chapter Rotor and access...

Страница 48: ...specially for the highest load speed temperature have a limited life time only and must to be replaced as recommended by the manufacturer Maximum permissible load difference The smaller the imbalance...

Страница 49: ...balance detection shut down as well as introduce significant detrimental wear to the motor and drive system When only partially loading the rotor ensure that oppo site bores receive tubes of equal wei...

Страница 50: ...Operation 46 Swinging bucket rotors These examples are to be applied to the other rotors in an analogous manner Proper loading Improper loading...

Страница 51: ...selection key to switch speed entry and display between rpm and RCF Bucket selection for swinging bucket rotors When running a swinging bucket rotor the automatic rotor identification feature will rec...

Страница 52: ...n the speed minimum 10 min 1 per snapping 3 After the required value has been achieved the decimal place is flashing several seconds and the display changes to the present value The speed has been sto...

Страница 53: ...es and adapters used You may take this into account when calculating the RCF value for your application Selecting run time There are two time modes standard and extended In the standard time mode you...

Страница 54: ...ates until you terminate it manually with Stop key Extended time mode As an option there is the possibility to change to the extended time mode Switching on respectively off this option is activated...

Страница 55: ...knob once again like for the speed selection see there Starting the centrifuge Once the rotor is properly installed the main switch turned on and the lid is closed you can start the centri fuge Press...

Страница 56: ...set run time Normally the run time has been selected and all you have to do is wait until the centrifuge terminates the run automatically at the end of the set time As soon as the speed reaches zero t...

Страница 57: ...of up to 4 K unacceptable you have to start the centrifuge for a short period of time until the rotor is identified Quick Run For short term operation the Legend is equipped with a Quick Run function...

Страница 58: ...then open the rotor e g in a safety work bench and decontami nate it Audible alarm Accompanying all error messages a warning signal is given out which only is silenced upon pressing any key You have t...

Страница 59: ...tion the cen trifuge is turned off The main power switch should be turned off after a complete centrifugation run Without motor deceleration it takes much more time until the rotor comes to a halt The...

Страница 60: ...Operation 56 Notes...

Страница 61: ...ake sure by consulting the manufacturer that the procedure foreseen does not cause any dam ages to the instrument Cleaning Pull mains plug before cleaning the instrument Clean the casing the rotor cha...

Страница 62: ...e disconnect the centrifuge from the mains supply Please pull mains plug Disinfection If a centrifuge tube containing infectious material leaks during a run you have to disinfect the centrifuge im med...

Страница 63: ...mains plug 2 Unscrew the rotor chuck 3 Grab the rotor with both hands and pull it perpen dicularly off the drive shaft 4 Remove the centrifuge tubes and adapters and disinfect them or dispose of them...

Страница 64: ...y autoclave the rotor and the adapters at 121 C Maximum permissible autoclaving cycle 20 min at 121 C The rotor must be cleaned and rinsed with distilled water before being autoclaved Remove the rotor...

Страница 65: ...ring it Inspection costs are charged as flat rate con tracts Necessary repairs are carried out free of cost within the warranty conditions and against payment after expira tion of the warranty period...

Страница 66: ...Maintenance and care 62 Notes...

Страница 67: ...Without power the brake does not function and deceleration takes much longer than normal Proceed as follows 1 Make sure that the rotor is at a stand still observe through window in the cover During a...

Страница 68: ...ord back into the instru ment and reinserting the plastic plug Once the power is restored you can connect the in strument to the main supply and turn it on Following the self test of the centrifuge th...

Страница 69: ...connection is OK contact KENDRO service Displays fail briefly The drive stops suddenly The rotor stops without deceleration Brief interruption of main supply 1 Turn off main switch 2 Check whether the...

Страница 70: ...or malfunction is responsible for the noise If you cannot locate and solve the problem yourself contact KENDRO service Message bAl appears in display Rotor stops with deceleration Imbalance switch act...

Страница 71: ...tion you must wait until the rotor has stopped By opening and closing the lid you reset the message rotor After entering a permissible speed you can press start Display OPEN appears although lid is cl...

Страница 72: ...sts contact KENDRO service E 15 E 16 Rotor stops with deceleration to standstill Instrument cannot be operated Temperature measurement error Switch the instrument off and on again If the problem persi...

Страница 73: ...be permitted for the operation in the respective rotor Please note the hints of chapter Bucket selection of swinging bucket rotors page 47 and compare the permitted rotors and rotor buckets in table 1...

Страница 74: ...ain switch E 22 E 23 Rotor stops without brake to standstill Instrument cannot be operated Error in speed measurement Switch the instrument off and on again If the error persists contact KENDRO servic...

Страница 75: ...r stops without brake to standstill or does not start Overtemperature in the motor 1 Turn instrument off and unplug mains power cord 2 Check and clean ventilation slots if necessary 3 After about 60 m...

Страница 76: ...iate circuit Switch the instrument off and on again If the error persists contact KENDRO service E 35 Rotor stops without brake to standstill or does not start Overcurrent in the intermediate circuit...

Страница 77: ...standstill Instrument cannot be operated Acceleration of rotor too slow Open the lid and check the load Significant imbalance caused by missing bucket Tubes or adapter do not fit rotor is rubbing the...

Страница 78: ...ument off 2 Keep the buckets selection key pressed and switch the instrument on For about 1 sec all displays read Subsequently the following readings will be dis played for 5 seconds each numbers are...

Страница 79: ...C 36 F to 95 F run time standard mode 1min 9 h 59 min hold permanent extended mode 1min 99 h hold maximum speed nmax 15 000 rpm rotor dependent adjustable in steps of 10 minimum speed nmin 300 rpm ma...

Страница 80: ...d T Legend RT 89 kg 196 lb 131 kg 288 lb Testing standards all devices manufactured and examined in agreement also for 120 V only for 230 V only IEC 61010 1 1990 amendment 1 1992 amendment 2 1995 IEC...

Страница 81: ...of instrument safety fuse thermic excess current release Fuse protection of building Legend T 7500 4366 120 V 60 Hz 10 2 A 900 W 15 A 15 AT Legend T 7500 4362 230 V 50 60 Hz 8 6 A 1250 W 10 A 16 AT Le...

Страница 82: ...78 Notes...

Страница 83: ...79 Appendix Deceleration profiles On the following pages you find deceleration profiles for each rotor type respectively...

Страница 84: ...80 0 1000 2000 3000 4000 5000 0 60 120 180 Deceleration profiles Swinging bucket rotor with rectangu lar buckets 7500 6446 speed rpm time s 1 3 2...

Страница 85: ...81 0 1000 2000 3000 4000 5000 6000 0 60 120 180 Deceleration profiles BIOshield Rotor 7500 6435 Highplate Rotor 7500 6444 speed rpm time s 1 3 2...

Страница 86: ...82 0 2000 4000 6000 8000 10000 12000 14000 16000 0 60 120 180 240 Deceleration profiles Micro liter Rotor 7500 3332 speed rpm time s 1 3 2...

Страница 87: ...83 0 1000 2000 3000 4000 5000 6000 7000 8000 9000 0 60 120 180 Deceleration profiles Highconic Rotor 7500 3057 speed rpm 2 3 1 time s...

Страница 88: ...84...

Страница 89: ...inging Bucket Rotor 7500 6445 7500 6434 10 100 1000 10000 100 1000 10000 RCF x g speed rpm Micro plate swing nmax 4600 rpm RCFmax 3761 Round bucket Rectangular bucket Multiple carrier nmax 4600 rpm RC...

Страница 90: ...86 10 100 1000 10000 100 1000 10000 nmax 5850 rpm RCF rmax nmax 6956 Speed RCF diagram BIOshield Rotor 7500 6435 RCF x g speed rpm...

Страница 91: ...87 10 100 1000 10000 100 1000 10000 nmax 5650 rpm RCF rmax nmax 5806 Speed RCF diagram Highplate Rotor 7500 6444 RCF x g speed rpm...

Страница 92: ...88 1 10 100 1000 10000 100000 100 1000 10000 100000 rmax 8 7 cm rmin 5 9 cm nmax 15 000 rpm RCF rmax nmax 21 885 Speed RCF diagram Micro liter Rotor 7500 3332 RCF x g speed rpm...

Страница 93: ...89 1 10 100 1000 10000 100000 100 1000 10000 100000 RCF x g speed rpm nmax 12 000 rpm RCF rmax nmax 16 260 rmax 10 1 cm rmin 6 3 cm Speed RCF diagram Angle Rotor 7500 3334...

Страница 94: ...90 1 10 100 1000 10000 100000 100 1000 10000 nmax 8500 rpm RCF rmax nmax 10 016 rmax 12 4 cm rmin 6 0 cm Speed RCF diagram Highconic Rotor 7500 3057 RCF x g speed rpm...

Страница 95: ...llation 13 changing settings during run 52 cleaning 57 closing the lid 41 collet chuck 42 common glass or plastic centrifuge tubes 4 conditions of warranty 61 contamination necessary measures 58 contr...

Страница 96: ...E 40 68 extendet time mode 50 F filling the centrifuge tubes 44 G general view 7 8 15 16 21 goes forward 51 H handling the centrifuge 4 hazardous substances 3 hazards symbols 5 hints symbol 5 hLd 49 I...

Страница 97: ...ermissible filling volume 38 maximum permissible RCF 51 maximum permissible speed 51 Maximum RCF value 16 maximum sample density 3 microtest plates 31 35 minimum distance from wall 13 O opening the li...

Страница 98: ...or identification 48 and RCF display 48 Rotor program for the Multifuge 16 rotors approved 42 run profile 7 run time range 49 selection 49 Run time selection 49 S safety instructions 3 safety measures...

Страница 99: ...ls for hazards and dangers 5 T Technical Data 75 test aerosol tightness 39 The printed documents consist of the delivery notes and this Manual 8 time counted forward in quick run mode 53 time mode ext...

Страница 100: ...96...

Страница 101: ...ro Laboratory Products International Sales Asheville NC USA Tel 1 828 658 2711 Fax 1 828 645 9466 info kendro spx com North America Kendro Laboratory Products Asheville NC USA Tel 1 800 879 7767 Fax 1...

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