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bright field observation, or the
pre-centred phase contrast slider
for phase contrast observation
(see section
7.1 ).
The aperture iris diaphragm is
closed or narrowed down too
much. Open the aperture iris diaphragm
(see section
7.2 ).
The dish holder or well plate
holder is blocking the light path. Move the dish holder or well plate
holder out of the way of the light
path (see section
4.2 ).
The objective is not correctly
engaged in the light path. Engage the objective in the light
path correctly (see section
7.3 ).
The light path is set to the
camera when trying to view
through the eyepieces, or vice
versa. Switch the light path to the visual
or camera light path (see section
7.4 ).
The umbra shield is down. Move the umbra shield up out of
the light path.
(Fluorescence observation) The
fluorescence mirror unit is not
properly engaged in the light
path. Engage the fluorescence mirror
unit in the light path correctly.
(Fluorescence observation) The
shutter for reflected light
illumination or the attenuator
filter is engaged in the light path. Engage the empty hole of the
filter slider for reflected light
illumination in the light path.
The fluorescence mirror unit in
use does not match the
specimen. Use the fluorescence mirror unit
matching the specimen.
The image is poor, or
the contrast of the
image is low, or the
resolution of the image
is poor, or the image
blurs. The container is fogged. Eliminate the fog.
The objective is dirty. Clean the objective.
The phase contrast slider is not
correctly engaged in the light
path. Match the phase contrast slider
correctly with the light path
position (see section
7.1 ).
(Fluorescence observation) The
umbra shield (ambient light Engage the umbra shield in the
light path.