iota Sciences isoHub, Руководство пользователя

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Non-clonal – Chamber contains either no cells or multiple cells. This is the default
state – a chamber is assumed to be
non-clonal
until it is marked as
clonal unfilled
via the Identify program.
Clonal unfilled – Chamber contains a single cell (or its derivative monoclonal
colony). Chamber volume is 200nl, indicating that the
Fill
program on the isoCell
has not yet been run on the dish.
Clonal filled – Chamber contains a single cell (or its derivative monoclonal colony).
Chamber volume is 600nl, indicating that the
Fill
program on the isoCell has been
run on the dish.
Detached – Dissociation reagent has been administered to the chamber using the
Detach
program on the isoCell.
Harvested – The colony has been harvested from this chamber using the
Extract
program on the isoCell.
(Most recently) Harvested – The harvested chambers produced by the most recent
run of the
Extract
program on the isoCell are distinguished by a small circle in their
corner. These chambers are the only ones that can be selected in the Prep
Redetach program.
Inactive – The cell or colony in this chamber is no longer active in the single-cell
cloning workflow. Chambers can be marked as
Inactive
in the Deactivate program.
Unknown – The chamber is in an unknown state. This can happen if, for example,
a program is cancelled while operating on the chamber. The
Recover
program on
the isoCell can be used to attempt to return these chambers to a
clonal filled
state.
4.5 Looking around a chamber
Whilst in a program, the joystick on the controller can be used to move to defined points in the
chamber to allow viewing the extremities of the chamber. When the joystick is released, the dish
holder will move back so that the chamber is centred in the field of view again.
The chamber should be centred in the field of view. If this is not the case, then you will
need to run through centre calibration (see section
4.10.3 ).