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Multitron Standard - Operating Manual

Operation

Page

70

of

114

17 March 2021

One-time change from phase 1 to 2

If the device is programmed using this method, the setpoint settings
for the cultivation process parameters will change when the specified
time expires. The device will then keep running using the setpoints
specified for the second phase until the device is stopped by deac-
tivating the parameters.

Example applications:



Starting the cultivation process on a time delay



Induction of a protein expression



Stopping or slowing down a cultivation process after a defined
period of time

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Содержание Multitron Standard

Страница 1: ...Multitron Standard Operating Manual 2021 03 17 We bring life to your laboratory...

Страница 2: ...Shaker Doc Nr 79325 EN V 05 00 Original This operating manual can also be found online at https www infors ht com en multitron standard Infors AG Headoffice Switzerland Rittergasse 27 CH 4103 Bottmin...

Страница 3: ...sons 13 2 5 Responsibility of the Provider 13 2 6 General Hazards 13 2 6 1 Electrical Current 14 2 6 2 Danger due to Moving Parts 14 2 6 3 Unauthorised Spare Parts and Accessories 14 2 7 Particular Ha...

Страница 4: ...2 Pre Fitted Trays 33 4 1 3 Tray with Sticky Stuff 38 4 2 Clamps and Other Holders 41 4 2 1 Clamps 41 4 2 2 Test Tube Holders 42 4 3 Box for Microtitre Plates 43 4 4 eve 46 5 Installation 47 5 1 Oper...

Страница 5: ...he Operating Hours Counter 79 6 7 Switching on the Interior Lighting 80 6 8 Switching off the Device 80 6 9 Behaviour in Case of Interrupted Power Supply 81 7 Rectifying Faults 82 7 1 Messages in the...

Страница 6: ...Interior Lighting 103 11 2 4 Materials 103 11 2 5 Emissions 103 11 2 6 Operating Conditions 104 11 2 7 Protection Type 104 11 2 8 Operating and Auxiliary Materials 105 11 3 Specifications of Paramete...

Страница 7: ...nt part of the device It must be kept near to the device and must be accessible to the operators at all times The users must read the operating manual thoroughly and fully un derstand its contents bef...

Страница 8: ...ded WARNING The signal word WARNING indicates a potentially dangerous sit uation that may result in severe or even fatal injuries if not avoided CAUTION The signal word CAUTION indicates a potentially...

Страница 9: ...2006 42 EC EMC Directive 2014 30 EU The Declaration of Conformity according to EC Machinery Directive 2006 42 EC annex II 1 A is attached to the operating manual see chapter 12 EC Declaration of Conf...

Страница 10: ...awareness of the associ ated risks 2 1 Intended Use Incorrect Use and Misuse Depending on its features the device is designed to be used as an incubation shaker for cultivating microorganisms or cell...

Страница 11: ...ve gases as well as operating the device in the Ex area are therefore not permitted The device is not designed to sufficiently protect its users if path ogenic organisms escape uncontrollably Cultivat...

Страница 12: ...rganisms of risk category 2 When cultivating pathogenic organisms of risk category 2 special measures must be taken to stop the organisms from escaping The user is responsible for this When using the...

Страница 13: ...rm user applies to all persons who come into contact with the device in any way and perform work on or with it This primarily applies to the following activities which can be performed by the manufact...

Страница 14: ...istry or biochemistry Life scientists biologists with special education in cytology bac teriology molecular biology genetics etc Lab assistants lab technicians from various fields In order to be class...

Страница 15: ...gulations applicable in a biotechnology laboratory are observed The provider must ensure that the device remains in safe and proper working condition throughout its entire term of use The provider mus...

Страница 16: ...ype Keep moisture away from live parts It may lead to a short cir cuit Never remove covers from the casing 2 6 2 Danger due to Moving Parts There is a risk of injury from moving parts Body parts can b...

Страница 17: ...of burns on hot surfaces in the interior or on the cultivation vessels For applications with temperatures over 55 C wear heat re sistant protective gloves 2 7 2 Dangerous Gases The use or production...

Страница 18: ...e it is nonethe less possible for pathogenic organisms and viruses to be cultivated Contact with pathogenic organisms bears a significant health risk Hence users are responsible for ensuring adequate...

Страница 19: ...l out the power plug before commencing any work on the electrical system Position On the left hand side of the top cooling system or on the front of the 31 mm base when using the base cooling system M...

Страница 20: ...contaminated before sending to the manufacturer The provider is therefore required to completely and truthfully fill out a declaration of decontamination and have it signed by the person responsible T...

Страница 21: ...3 1 Setup of the Basic Unit Outside 1 Casing 2 Operating panel 3 Serial interface RS 232 not visible 4 Door handle 5 Identification plate 2x 6 Door with window opens downwards 7 Discharge outlet 8 Rub...

Страница 22: ...ate micro organisms in a laboratory environment The basic version of the de vice is fitted with a shaker drive and a heater Depending on the ver sion of the device it will come with either a 25 mm or...

Страница 23: ...throw of 25 or 50 mm and with or without a cooling system The tables below provide an overview of the configurations that are available as standard Individual Units Individual unit with 25 or 50 mm th...

Страница 24: ...its stacked with 25 or 50 mm throw with out cooling base 31 cm Two units stacked with 25 or 50 mm throw with side cooling system base 31 cm Three units stacked Three units stacked with 25 or 50 mm thr...

Страница 25: ...tion flasks the drive is switched off automatically as soon as the door is opened The counterweight used to balance the mass is fitted under the table Depending on the design the deflection of the cir...

Страница 26: ...ading to increased wear on the bearings and joints The permissible load weights depend on the position of the device in the stack the throw and the shaking speed see chapter 6 2 6 Tips Tricks for Load...

Страница 27: ...tures below the ambient temperature the device can be fitted with an optional cooling system see chapter 3 3 3 Op tional Cooling Function page 26 A Pt100 temperature sensor behind the rear wall 2 not...

Страница 28: ...its the cooling unit is installed in the 31 mm base The cooling unit is a 2 circuit system with a cooling liquid tank which must be filled with cooling liquid by INFORS HT prior to commission ing Side...

Страница 29: ...ins connection is located on the left side of the device to wards the rear Two different versions of the device are available for different mains voltages 230 V 50 60 Hz 115 V 60 Hz Two fuses immediat...

Страница 30: ...l status 4 Reserve 1 7 Alarm and power failure 5 GND 2 RX receiver 8 Reserve 3 TX transmitter 9 Reserve 3 6 Openings 3 6 1 Discharge Outlet The opening for discharging leaked liquids detergents or con...

Страница 31: ...bber Feet 3 cm Individual units placed on a bench have a substructure comprising an earthing plate with 7 rubber feet The rubber feet are bolted onto the earthing plate at a fixed height and cannot be...

Страница 32: ...iately when it is switched on via the power switch When the device is switched off using the power switch the pa rameter setpoints are stored for approx one month 3 8 2 Operating Panel All device func...

Страница 33: ...d side of the device next to the mains connection A second identification plate is located at the bottom of the door For information on the data specified on the identification plate see chapter 1 3 D...

Страница 34: ...al Table Tray The M tray referred to as universal table tray with the dimensions 85 x 47 cm features drill holes on a grid so that it can be fitted as de sired For fitting various clamps and test tube...

Страница 35: ...heir holders cannot be changed Pre fitted trays feature a capacity that is up to 20 higher than an individually equipped universal table tray Since no drill holes have to be taken into account in the...

Страница 36: ...2000 ml 13 3000 ml 9 4000 ml 8 5000 ml 6 Tray with pin holders For applications in which large numbers of microtitre or deep well plates need to be incubated various trays with pin holders are availa...

Страница 37: ...dividual plates or stacks of plates Quickload spacers are long plastic strips 4 mm high that span across several plates thus allowing you to save time while loading ATTENTION The spacers are made of p...

Страница 38: ...s on the edge 1 for positioning microtitre plates or deep well plates Tray with clamping assemblies tilted at an angle of 20 angled incu bation The tilted clamping assembly is a wedge shaped metal she...

Страница 39: ...lid ing bars can be affixed to any point on the support bars This also al lows you to affix bottles with vertical walls and a curved base The bars are sheathed in a soft material and arranged on two d...

Страница 40: ...in the fridge warm up to ambient temperature first before placing them on the ad hesive matting If you are looking for a very flexible option for equipping the tray Sticky Stuff offers an ideal alter...

Страница 41: ...e short neck Fernbach flasks in particular can be difficult to remove If necessary cover part of the adhesive matting with the protective foil provided The adhesive power can deteriorate over time due...

Страница 42: ...ing the maximum permissible speed is limited when using adhesive mat ting INFORMATION The following guidelines only apply to undamaged completely dry adhesive matting and flasks free of grease If old...

Страница 43: ...mounted on the universal table tray Stainless steel clamps The following stainless steel clamps are available For flask type Volume Erlenmeyer 25 mL 500 mL 4000 mL 50 mL 1000 mL 5000 mL 100 mL 2000 m...

Страница 44: ...ng The following test tube holders are available details and special types on request For long test tubes 8 bis 30 mm For short test tubes 12 bis 18 mm For plastic tubes with ventilation lid 16 and 30...

Страница 45: ...ich a replaceable paper filter is integrated A microtitre insert with screw mounted pins is used for mounting mi crotitre plates and deep well plates For shaking the box for microtitre plates can be s...

Страница 46: ...wing that the filter can be inserted and the filter washer can be put back in its place Sterilising the box for microtitre plates The box for microtitre plates can be autoclaved recommendation 20 min...

Страница 47: ...bular Screws for microtitre box M4 x 12 Phillips head Material Aluminium autoclavable Detergent Mild dishwashing liquid or neutral cleaning agent Quickload spacer Description Value Dimension 412 x 14...

Страница 48: ...can be generated In addition to INFORS HT products biotech equipment and analysis devices from third party manufacturers can also be integrated In this way bioprocesses can be controlled monitored an...

Страница 49: ...ty Only the manufacturer s expert personnel or person s author ised by the manufacturer may install and initially commission the device The manufacturer must also be consulted if the device is sub seq...

Страница 50: ...the desired temperature in the incubation chamber the ambient temperature directly at the device is deci sive This can be considerably higher than the temperature at other positions in the room due to...

Страница 51: ...en placing the device on a bench observe the following points To prevent the bench from vibrating use a heavy bench with diagonal braces as the minimum ideally use a stable labora tory bench Alternati...

Страница 52: ...bench to ensure that heat pro duced by the device can escape The minimum distance between the device and the underside of the bench top is 100 mm ATTENTION Failure to comply with the required minimum...

Страница 53: ...ll automatically run a self test The letters INI initialisation will appear in the view box on the right and light up briefly Once the initialisation process is complete the Power supply on off functi...

Страница 54: ...N If the door is opened when the shaker drive is running the table can cause injury due to its significant momentum As such take care when opening the door while the shaker drive is running and wait u...

Страница 55: ...cylindrical ball casters inside them 1 which are located on the inside of the door Two screw mounted pins 3 in the slide rails limit how far the tray can be pulled out When pulled out all the way the...

Страница 56: ...uide rails 3 over the front stop bar 2 and up to the stop 1 in the incubation chamber keeping it straight The tray must audibly click into place behind the front stop bar 5 Check whether the tray is s...

Страница 57: ...l not possible to insert the tray correctly contact your nearest INFORS HT representative 6 2 4 Handling Cultivation Flasks without Removing the Tray The cultivation flasks can also be handled without...

Страница 58: ...ter plates on the Sticky Stuff adhesive matting Fitting a holder Clamps test tubes and boxes for microtiter plates are fixed to the tray using screws The device is delivered with pre fitted screws 2 T...

Страница 59: ...pecification below Steel clamps Flask size Screw Flat gasket 25 up to 50 mL Flat head screw with Phil lips head M4 x 6 A4 D 3 2 x 12 x 0 5 100 up to 5000 mL Flat head screw with Phil lips head M4 x 8...

Страница 60: ...If any of the vessels protrude over the edge of the tray they could collide with the inner wall resulting in the device being damaged or vessels breaking As such always ensure that the cultivation ve...

Страница 61: ...ls into the following ranges mass incl tray clamps flasks and filling Throw Speed min 1 Up to 250 250 or higher Up to 350 350 or higher 25 mm 9 19 kg 12 16 kg 50 mm 9 19 kg 12 16 kg The loads listed a...

Страница 62: ...function button 6 Plus button 7 On Off button 8 Minus button 9 FT time delay function button 10 Timer parameter symbol 11 Temperature parameter symbol 12 Setpoint speed parameter symbol 13 External EX...

Страница 63: ...FT button The FT button can be used to select the device s parameters one after the other so that they can be adjusted for the next phase The FT but ton is only ever used in conjunction with the time...

Страница 64: ...r function length of time unit can be adjusted External EX Indicates that the device is being accessed by an external device This applies when the device is being controlled using the operating panel...

Страница 65: ...operation the current actual value for the Speed RPM parameter is displayed whenever this parameter is active Oth erwise the display will alternate between the set setpoint and the message OFF Right...

Страница 66: ...tes to 9 hours 50 minutes The time is displayed in the following format hours h minutes x10 Example 9 hours 50 minutes From 10 to 95 hours The time is displayed in hours h Example 95 hours From 4 days...

Страница 67: ...rature setpoint in degrees Celsius is displayed to one decimal point The adjustment range is between 0 and 65 C INFORMATION The temperatures that can actually be achieved depend on a vari ety of facto...

Страница 68: ...the pa rameter If you do not input anything within 2 seconds the dis play will alternate between the set setpoint and the messages ON and OFF 2 Press the Plus or Minus button to set the setpoint you...

Страница 69: ...tpoint and the message OFF 2 Press the On Off button 3 to activate the parameter The parameter will be activated immediately The right hand dis play will alternate between the current actual value for...

Страница 70: ...peed RPM parameter is activated or deactivated the following messages will appear in the right hand view box The Sta Start message appears when the shaker drive is switched on and goes out when the sp...

Страница 71: ...ot activated the device runs until it is stopped by manually deactivating the parameters 6 5 1 Overview The timer function can be used to set defined sequences Two differ ent programming modes are ava...

Страница 72: ...ion process parameters will change when the specified time expires The device will then keep running using the setpoints specified for the second phase until the device is stopped by deac tivating the...

Страница 73: ...f the device is programmed using this method two different param eter settings will be repeated on an endless precisely timed cycle The two intervals phase 1 and phase 2 alternate until the cultivatio...

Страница 74: ...T button and activate the parameters c Set and activate the timer using the F button Setting the Setpoints for Phase 1 F Button Procedure 1 Press the F button 1 to select the parameter in question RPM...

Страница 75: ...on 3 to set the setpoint you want to use for the selected parameter 7 Press the On Off button 4 to activate the parameter 8 Repeat steps 5 7 as necessary for the other parameters Setting the Timer F B...

Страница 76: ...or the second phase until it is switched off manually or the setpoints are modified INFORMATION The timer function can be deactivated at any time by switching off the timer The device will then contin...

Страница 77: ...tton 3 to activate the parameter 4 Repeat steps 1 3 as necessary for the other parameters INFORMATION For details of how to set the parameters see chapter 6 4 Adjust ing Activating and Deactivating Pa...

Страница 78: ...the setpoint you want to use for the selected parameter 10 Press the On Off button 4 to activate the parameter 11 Repeat steps 8 10 as necessary for the other parameters Setting the Timer for Phase 2...

Страница 79: ...sh for the duration INFORMATION The timer function can be deactivated at any time by switching off the timer The device will then continue running with the last ac tive parameter settings 6 5 4 Displa...

Страница 80: ...ymbol 2 will light up The remaining time will be displayed in the right hand view box 2 Deactivate the timer using the On Off button 4 The Timer active function symbol 1 will go out The device will no...

Страница 81: ...and view box displays hours 0 to 999 To activate the operating hours display proceed as follows Procedure 1 Press and hold the F and FT buttons 2 and 3 at the same time The Timer active function symbo...

Страница 82: ...s To do this press and hold the F button When the F button is released the lighting will switch off again 6 8 Switching off the Device ATTENTION If the parameters are not deactivated before the device...

Страница 83: ...ce is interrupted during a cultivation process e g by flicking the power switch or in case of a power fail ure all the parameters and timer setpoints will be stored together with the time remaining on...

Страница 84: ...to technical faults in the device Depend ing on the type of the error in question either the device or indi vidual components will be brought to an immediate stop In such cases it will not be possibl...

Страница 85: ...rature meas urement Check whether the Pt100 sensor is working properly In case of a defect contact your INFORS HT representative Operator Heater fan not working Contact your local INFORS HT representa...

Страница 86: ...d Switch the device off and then on again Operator Control system defective Contact your local INFORS HT representative INFORS HT service tech nician or licensed dealer Alarm message Meaning The power...

Страница 87: ...ally and visually If an error occurs the text Err Err1 or Err2 will be displayed in alter nation with the actual value of the parameter Error messages can be confirmed by pressing the F button The fol...

Страница 88: ...INFORS HT service tech nician or licensed dealer Error message Meaning No value shown on temperature display Possible cause Measures Personnel Pt100 sensor or sensor cable defective Contact your local...

Страница 89: ...again Operator Door mechanism defective Contact your local INFORS HT representative INFORS HT service tech nician or licensed dealer Fault The tray is not released when the door is opened Possible cau...

Страница 90: ...ler The drive control system is de fective Contact your local INFORS HT representative INFORS HT service tech nician or licensed dealer Fault Strong vibrations occur Possible cause Measures Personnel...

Страница 91: ...nt temperature and correct if nec essary see chapter 5 1 Operating Conditions at the Installation Location page 47 Operator Door not fully closed Close the door completely Operator Cooling fins dusty...

Страница 92: ...e the defective fuse 4 Insert a new fuse with the correct number of Amperes 5 Push the plug as far back in the opening as possible until it snaps in 6 Re establish the power supply to the device 7 3 2...

Страница 93: ...ce department of the local dealer or the manufacturer on site diagnosis and or repair is not possible INFORMATION When returning the device the component part or accessory for repair it is required fo...

Страница 94: ...more or less corrosive effects on the metal parts When using particularly ag gressive substances more frequent checks are required in order to maintain smooth device operation Contact the manufacturer...

Страница 95: ...2 ATTENTION Insufficient cleaning and disinfection can lead to damage to cul tures due to contamination 8 2 1 Cleaning Detergent Mild detergents e g dishwashing liquid or neutral cleaning agents are s...

Страница 96: ...follows ATTENTION After being folded up the table must be held in position by hand If the table is not secured properly there is a risk of it folding back down again This can cause injuries If possibl...

Страница 97: ...ed make sure that they are always replaced using original screws M5x16 hexagonal socket 8 2 2 Disinfection Only use quaternary ammonium compounds for wipe down disinfec tion As a tried as tested disin...

Страница 98: ...e device transport fasteners rubber wedges must be inserted to prevent uncontrolled movements of the ta ble Always work in pairs and use suitable auxiliary equipment when transporting the unit Especia...

Страница 99: ...nt Refer to main chapter Safety and Responsibility chapter Decla ration of Contamination for details 10 1 Disassembly Prior to disassembly Switch off the device and lock any isolation switch in the of...

Страница 100: ...materials supplies are subject to hazardous waste regulations and may only be disposed of by registered specialist disposal firms For disposal the system units are to be disassembled and dismantled in...

Страница 101: ...andard Operating Manual Technical Data and Specifications 17 March 2021 Page 99 of 114 11 Technical Data and Specifications 11 1 Dimension Drawings Individual unit with table feet Individual unit with...

Страница 102: ...andard Operating Manual Technical Data and Specifications Page 100 of 114 17 March 2021 Two units stacked with 31 cm base and side mounted cooling system Three units stacked with 13 cm base and top co...

Страница 103: ...h door closed 870 mm Depth with door open 1160 mm Height 530 mm Height with rubber feet 550 mm Height with 13 cm base 665 mm Height with 31 cm base 845 mm Exterior dimensions for two units stacked Des...

Страница 104: ...scription Type 230 V Type 115 V Unit Value Voltage 230 115 VAC Frequency range 50 60 60 Hz Electrical performance values Description Type 230 V Type 115 V Unit Value Power max 880 880 W Power consumpt...

Страница 105: ...115 V 60 Hz Power max 220 220 200 W Power consumption 1 2 1 2 2 6 A Fuses Two 5 x 20 mm fuses time lag 10 10 16 A 11 2 3 Interior Lighting Description Value Unit Type 2 halogen lamps with G4 pin base...

Страница 106: ...ocation max 2000 m a s l Pollution degree according to EN 61010 1 2 Min distance from walls ceilings and other devices 100 mm INFORMATION The specified temperature range refers to the temperature dire...

Страница 107: ...ample Antifrogen L General detergent Mild neutral cleaning agent Detergent for adhesive matting Mild neutral cleaning agent Dishwashing detergent Disinfectant Quaternary ammonium com pounds 11 3 Speci...

Страница 108: ...ystem Sensor type Pt100 Measurement range of the sensor 4 80 C Capillary thermostat control preci sion 2 5 C Default thermo switch setting for capillary thermostat 70 C Bi metallic heating switch appr...

Страница 109: ...lower than for single units Individual units Throw Value 25 mm 20 to 400 min 1 50 mm 20 to 350 min 1 Two units stacked Device 25 mm throw 50 mm throw Bottom unit 20 to 400 min 1 20 to 350 min 1 Top un...

Страница 110: ...n the stack As such the following guideline values only apply to individual units no guarantees In or der to reach these guideline values the device must be free standing and the heat it generates mus...

Страница 111: ......

Страница 112: ...astic 42 stainless steel 41 Cleaning base tray 94 Sticky Stuff 39 Commissioning 47 Conditions at the installation location 47 at the storage location 96 Connection values electrical 102 Coolant 105 10...

Страница 113: ...5 Fault messages 85 Faults 82 general faults 87 shaker drive 85 88 temperature control 86 89 Fernbach flasks requirements 9 Flasks 9 Flip switch 30 Frame 29 FT button 61 Functional description cooling...

Страница 114: ...es 49 Minus button 61 Misuse 8 Motor operating hours 79 specification 105 Moving 47 O On Off button 61 Opening the door 52 Operating conditions 47 104 Operating elements 61 Operating hours counter 79...

Страница 115: ...two units stacked 107 with microtitre box 44 with Sticky Stuff 40 RS 232 28 Rubber feet 29 S Safety features 16 Screw sizes holders 57 table 94 Serial interface 28 Servicing 92 Setpoint achievable tem...

Страница 116: ...5 Time information 64 Timer function aborting 78 cyclical change 71 displaying the remaining time 77 interrupted power supply 81 one time change 70 programming a cyclical change 74 programming a one t...

Страница 117: ...tform Software Able to do more than just plan control and analyze your bioprocesses eve software integrates workflows devices bioprocess information and big data in a platform that lets you organize y...

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