DeltaMyc Operation Manual J810018 (7 Oct 2014)
Operation
3-31
7
If the results are reasonable, you can click the
Use for all button to continue the fit with the oth-
er decays with similar parameters.
The time required to fit the data depends on the number of decays and the num-
ber of exponential components selected.
8
Speed up the process by deactivating the Dis-
play updates checkbox.
When the fit completes, it appears in the fit tree. You can view the fit results in
the left bottom part of the screen. (To get a better view of the values, select
Open in window.)
9
Assess the goodness of fit.
This is best done in terms of the weighted residuals (indicates how the fitted
function differs from the data) and a reduced
χ
2
value:
CHISQ
or
XSQ
. Other
indicators are also provided.
The usual values of interest are the lifetime (
T
value), its error (recommended to
be taken as 3
σ
, i.e., three standard deviations) and the pre-exponential (
B
) value,
which indicates the relative weight of the lifetime. This pre-exponential can be
represented in two ways: as a relative amplitude (
Rel. Ampl.
), in which the pre-
exponential is weighted by the lifetime: useful when comparing with steady
state spectra; and by normalizing these (
B
) values: useful when performing in
depth analysis on multi-component systems. Finally, the goodness of fit, repre-
sented by the
CHISQ
value.
10
Display the fit-parameter maps by clicking the
Map icon at the top of the main window.
The
2D Microscope Map
window opens.
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