DeltaMyc Operation Manual J810018 (7 Oct 2014)
Operation
3-22
If you stored named positions during a previous single-point sequence, you can
reach them again by selecting the appropriate position in the list and clicking
the
Go to position
button.
7
Save the live picture as a bitmap file at any time
by clicking the Save picture to BMP file button.
8
When the sample is properly positioned, click
OK to validate and return to the main DataSta-
tion window.
Now the measurement tree is initialized, and shows a
Photos
folder and a
Click
here to begin
item (Fig. 22). The
Photos
folder contains the picture of the
sample in its anchor location.
Fig. 22. Measurement tree after the anchor has been selected.
Run the microscope scan wizard.
The set-up parameters and the measurement locations on the anchored sample are con-
figured using the microscope scan wizard.
1
Click the Click here to begin item in the meas-
urement tree.
The wizard window is displayed (Fig. 23).
Figure 23: The microscope scan wizard – Measurement parameters
Prompt parameters
You may measure one prompt if you will analyze the decay data later using re-
convolution. You can measure the prompt after or before the decays. The Ac-
quisition presets define the duration of the measurement. At least one preset
must selected. For the prompt acquisition, it is appropriate to select
None
or
Beamsplitter
in the edge-filter drop-down list.
Decay parameters
Similarly, the acquisition preset must be specified for the decays. If you are un-
sure how long a measurement will take, we recommend a runtime preset, be-
cause some points of the sample may be of low intensity. Select the appropriate
edge and emission filters for your sample.
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