Horiba Scientific DeltaMyc J810018, Руководство по эксплуатации

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DeltaMyc Operation Manual J810018 (7 Oct 2014) Operation
3-2
Controlling the hardware
Control of the hardware is in the lower part of the tree in the DataStation main window.
Neutral-density (ND) filters
Neutral-density filters are manually selected.
Pinholes
The system is fitted with your choice of pinholes. The fluorescence emission beam is
focused on one of these pinholes, in order to filter out the background fluorescence.
Pinholes are manually selected.
Microscope mode
The Microscope mode item in the tree allows switching between Imaging mode and
Time-resolved mode. These two modes define the two possible optical paths in the life-
time head, which allow:

Imaging
In this mode, the top CCD camera can be used to display live video im-
ages of the sample. The microscope lamp is switched on and its intensi-
ty can be varied. You can select a suitable filter-cube in the illuminator
and use the binoculars/top camera to view/image the sample. The laser
is only on when the camera-only position. Note that, in this position, the
laser beam is blocked before reaching the sample, so there is no laser
safety hazard when viewing through the binoculars. An interlock cable
goes to the DeltaDiode controller. If this cable is removed, the laser will
not operate.

Time-
resolved
In this position, the laser beam is directed to the sample (view through
the binoculars/camera is blocked). The emission beam is directed to the
TCSPC detector. Time-resolved acquisitions are possible.
Note that the factory default is that the first position in the illuminator contains no filter
cube, and the second position contains a half-mirror cube. Therefore position
1
is suita-
ble for time-resolved measurements and position
2
is suitable for imaging mode.
Caution:
Attenuate or block any unnecessary light
sources to avoid damage to the detector.