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Prime System… Fluid Settings:
The values entered in this section specify the volume, source, and rate
of flow when using the functions found in the following section “Pump Operations”. The Fluid volume
value is limited by the size of the currently installed syringe. The Fluid source can be toggled between
the flow cell and the waste container. The Flow rate is determined by the user and is in milliliters per
minute.
Prime System… Pump Operations:
The Aspirate and Dispense functions will either draw from or
send to the specified fluid source the entered fluid volume at the specified flow rate. The Fine Control
section Aspirate and Dispense functions operate in a similar manner except that the fluid volume is
preset to the minimum volume allowed by the configuration of the syringe pump. The “Stop Pump”
function will cause the syringe pump to immediately stop the movement of the syringe plunger. The
“Evacuate Syringe” function will cause the syringe pump to fully empty the contents of the syringe to
the waste container.
Flush System:
The user can enter the Number of Cycles and the software will calculate the Time
Remaining to complete the specified cycles. The Current Cycle is displayed and the process can be
stopped at any time by clicking the “Cancel” button.
The checkbox labeled
“Automatically send sample to waste bottle”
is an optional setting that when
enabled instructs the FlowCAM to evacuate any remaining sample from the system to the waste
container at the end of each run. Note that the volume of sample to evacuate is partially calculated from
the length of the lower tubing entered in the Context > Context Settings > Flow Cell (tab).
Setup Menu… Setup and Focus… (F6)
Opening the Setup and Focus window shows the entire field of view of the camera. This view is ideally
used for image focusing and for viewing the image brightness.
Pause Button:
Selecting this button will cause the camera to stop capturing images of the field of view.
This will enable the Save Image… function described below in the File Menu section.
Resume Button:
Selecting this button will restart the camera capturing images of the field of view in
real time.
Z+ and Z- Button:
The user can toggle between these two buttons to zoom in (+) or zoom out (-) on
the camera field of view currently displayed in this window. The zoom in function may be useful during
fine focus adjustments. Note that the Z+ and Z- buttons appear in other places in the VisualSpreadsheet
software and these buttons have the same zoom in and zoom out functionality.
