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Color Coordinates for U (X-Axis) and V (Y-Axis) Settings
Gamma, Hue (if available), and Saturation (if available)
- These are inter-related adjustment
parameters that can be used to fine tune the color of the camera field of view (after the major factors
White Balance U and White Balance V have been adjusted). In general, these settings will not need to
be adjusted by the user.
Sharpness (if available)
- This parameter employs an algorithm to increase the resolution of the gray
scale. The value for this parameter indicates the number of iterations of this algorithm that are applied.
Color Gain
(if available)
- Relates to the gain level for the overall color setting for a color camera.
Red, Green, Blue Gain (if available)
- The individual gain levels for each color for a color camera.
Timing
AutoImage Rate
- The user can select a camera rate (frames per second) to use while running in
AutoImage Mode. The AutoImage Rate is typically used in conjunction with the pump flow rate to
minimize multi-imaging of particles.
Flash/Camera Delay
- This parameter is the period of time (in microseconds) between the point when
the camera is initially instructed to take an image, and the moment when it actually triggers the flash and
captures the image. This value is generally not adjusted and is left set to the factory default.
Flash Duration
- This value corresponds to the number of microseconds that the Flash LED will be on,
providing illumination to the sample in the flow cell. This parameter, along with Gain, is commonly
used to adjust the amount of light passing through the flow cell to achieve optimal illumination. In
general, Flash Duration should be set to the lowest value possible that still provides optimal illumination
when assessed in combination with Gain and the remaining camera settings.
Quadrant II – Orange
Quadrant III – Green
Quadrant I – Purple
Quadrant IV – Blue
