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LEXCELL

NTERNATIONAL

ORPORATION

TABLE OF CONTENTS

One Year Limited Parts Warranty ................................................................................................ ii
Dell Computer Warranty Information ......................................................................................... iii
Federal Communications Commission Radio Frequency Interference Statement ...................... iv
FX-5000

™

Quick Start Instructions .............................................................................................. v

1. Flexcell

FX-5000

™

Tension System Components

.................................................... 1

2. Setup of the Flexcell

FX-5000

™

Tension System

..................................................... 1

2.1

Packing

List

...................................................................................................................... 1

2.2. Location of the FX-5000

™

Tension System .................................................................... 1

2.3 Setup of the Computer and FX5K

™

Tension FlexLink

.................................................. 2

2.4 Incubator Access Ports ...................................................................................................... 3

2.5 Setup of the BioFlex

Baseplate(s) with Loading Stations

™

and Culture Plates ............. 4

2.6 Connecting the System Vacuum Lines ............................................................................. 4

2.7

FlexSoft

FX-5000

™

Software and System Power-up ..................................................... 5

2.8 LCD Display on the FX5K

™

Tension FlexLink

............................................................. 6

2.9 Using Additional FX5K

™

Tension FlexLinks

................................................................ 6

3. Operation Instructions

........................................................................................................ 7

3.1 Starting the FX-5000

™

Program ....................................................................................... 7

3.2. Using the FX-5000

™

Tutorial .......................................................................................... 7

3.3 Main Display Window ...................................................................................................... 7

3.4. Configuring Users ............................................................................................................ 9

3.5 Creating Regimens .......................................................................................................... 10

3.6 Creating a Regimen with a Custom Waveform Shape ................................................... 11

3.7 Assigning Regimens ....................................................................................................... 12

3.8

Starting

Regimens

........................................................................................................... 13

3.9 Simulating Regimens ...................................................................................................... 13

3.10 Pausing Regimens ......................................................................................................... 13

3.11 Resuming Regimens ..................................................................................................... 13

3.12 Stopping Regimens ....................................................................................................... 13

3.13 Determine the Progress of the Regimen ....................................................................... 13

3.14 Resetting the FlexLink

................................................................................................ 14

3.15 Controlling the Vacuum Pump ..................................................................................... 14

3.16 Data Analysis ................................................................................................................ 14

3.17 Shutting Down the System............................................................................................ 15

3.18 Exiting the FX-5000

™

Program .................................................................................... 16

4. Addendums to the FX-5000

™

Tension System

.......................................................... 16

For Optimal Performance of the Tension Unit ..................................................................... 16

5. System Troubleshooting

.................................................................................................. 17

6. Application Notes

.............................................................................................................. 20

Baseplate and Gasket Cleaning and Disinfecting ................................................................. 20

Running Low Frequency Regimens with BioFlex

Culture Plates ...................................... 20

7. Waveform Shape Definitions

......................................................................................... 21

8. Flexcell

FX-5000

™

Tension System Specifications

............................................... 22

Appendix: FX-5000

™

Tension System Dual Baseplate Operation

............................ 25

Содержание FX-5000

Страница 1: ...21 0 Culturing Cells in a Mechanically Active Environment Flexcell International Corporation 2730 Tucker Street Suite 200 Burlington NC 27215 800 728 3714 919 732 1591 FAX 919 732 5196 www flexcellin...

Страница 2: ...uctions 7 3 1 Starting the FX 5000 Program 7 3 2 Using the FX 5000 Tutorial 7 3 3 Main Display Window 7 3 4 Configuring Users 9 3 5 Creating Regimens 10 3 6 Creating a Regimen with a Custom Waveform S...

Страница 3: ...than our factory or distributor Breaking the seal and opening the unit case or violating unit components will void the warranty Failure to run the drying regimen as recommended in this manual will vo...

Страница 4: ...nty are listed below You will need to have the service tag number and Express Service Code number which are located on the side of the computer Dell Hardware Limited Warranty Plus Onsite Service Exten...

Страница 5: ...to a properly grounded power outlet and used with the power cord supplied with the unit s unmodified 3 The unit must be operated with the factory installed cover in place 4 No modifications of the equ...

Страница 6: ...seplate s in the incubator a Insert the 6 4 mm and 9 5 mm tubing into the access opening of the incubator b Connect the quick disconnects for the 6 4 mm and 9 5 mm tubing to the baseplate s c Place fo...

Страница 7: ...D for vacuum source connection 16 Flexcell FX 5000 Tension System user s manual and other ancillary manuals for computer and monitor 17 Surge protected outlet strip 18 Water Trap x2 19 Vacuum source o...

Страница 8: ...the system is 48 37 lbs 21 96 kg Figure 3 Dell OptiPlex rear view 2 3 SETUP OF THE COMPUTER AND FX5K TENSION FLEXLINK Setup the Dell OptiPlex desktop computer and peripherals according to the manufac...

Страница 9: ...onto the fitting Tighten each nut by hand until the tubing is crimped to minimize vacuum leakage Connect the power cables 115 VAC or 230 VAC to the rear power inlet FlexLink Power of the FlexLink Fig...

Страница 10: ...ure that the gaskets are fully pressed onto the culture plates and that no parts are folded under 4 Place the culture plates and gaskets into the baseplate wells Check to be sure that the gaskets are...

Страница 11: ...use the auto control pump feature plug the vacuum pump power cable into the PUMP CONTROL CONNECTION outlet on the rear of the FX5K Tension FlexLink Fig 2 and 7 NOTE When using the auto pump control fe...

Страница 12: ...imen Ready User name Platform Regimen Name Flexcell International Corp www flexcellint com Regimen is running or being simulated 1 Regimen Left 5 min User name Platform Regimen Name Flexcell Internati...

Страница 13: ...ength as much as possible between the vacuum source and the baseplate See page 16 for more information on obtaining the optimal performance of your FX 5000 Tension System 3 1 STARTING THE FX 5000 PROG...

Страница 14: ...al data patterns may be confounded with noise For the case of the pressure plot as a function of voltage from the FX 5000 Tension System pressure transducer the rough data yield a plot with a coeffici...

Страница 15: ...changed by clicking on one of the two small double arrow buttons at either end of the axis The button with arrows pointing away from each other increases the axis scale while the button with arrows po...

Страница 16: ...boxes under Min and Max 4 Enter the frequency for the waveform 0 01 5 Hz in the box under Freq 5 If a static regimen is required enter the desired elongation at which the membrane is to be held in the...

Страница 17: ...ed regimen name in the Regimens for Selected User box and then click on Delete Regimen Confirm the deletion by clicking on Yes NOTE If the regimen to be deleted is currently assigned the regimen must...

Страница 18: ...ll be MAXIMUM and the regimen cannot be saved Adjust the time and or cycle number to decrease the total duration time 7 Click on Save Regimen 8 When finished with regimen editing click on Close 3 7 AS...

Страница 19: ...unusual elements is to accommodate for the lag time during sudden changes in pressure e g rising falling edge of a square wave Without these unusual elements the valve controllers may oscillate for a...

Страница 20: ...l turn the pump ON The software will turn the pump OFF when a regimen is complete This option also allows the pump to be turned OFF during a regimen step when a static waveform regimen is selected tha...

Страница 21: ...box can be set so that the data viewed in the plot begin at the specified time entered here Use the timeline at the top of the graph to navigate through the regimen Users can move the slider function...

Страница 22: ...the incubator and stored flat to prevent warping Do not hang the red gaskets on pegs to dry They will stretch over time 3 18 EXITING THE FX 5000 PROGRAM To exit the FX 5000 software in the Controller...

Страница 23: ...elongation and frequency a Shorten the FLEX IN FLEX OUT and SYSTEM tubing to their minimum possible lengths See section 2 2 for minimum lengths b Use a more powerful vacuum source if recommended spec...

Страница 24: ...Regimen wave is flatlining Solution 2 Remove the SYSTEM vacuum line at the back of the FlexLink and check to be sure that there is vacuum coming through the line Check the VENT port at the back of the...

Страница 25: ...of the cells in the middle of the well due to gravity or b incomplete trypsinization or over trypsinization of cells causing clumping and uneven distribution of cells Solution 8 a Use 2 3 ml of media...

Страница 26: ...y dry before connecting to the FX5K Tension FlexLink Contact time time surface remains wet of the above solutions is critical to the effectiveness of disinfecting Times given are minimal and longer co...

Страница 27: ...re from min to max Triangle Wave This waveform provides a linear ramped rise to the max and a linear ramped fall back to the min Square Wave This waveform provides the fastest possible rise to max fol...

Страница 28: ...inusoidal wave Heart wave Electronic and Pressure Triangular wave Square wave and custom waveforms Provides equibiaxial strain or unconstrained distension to the BioFlex culture plate membrane Optiona...

Страница 29: ...m design vacuum controller board o Microprocessor sampling rate for display data transducer 200 Hz o Microprocessor sampling rate for feedback error valve adjustment transducer 1 KHz 2 proportional so...

Страница 30: ...equibiaxial strain Minimum achievable elongation with the FX 5000 1 2 Maximum achievable elongation with the FX 5000 8 0 FX 5000 TENSION PHYSICAL SPECIFICATIONS FX 5000 Computer Size W x H x D 3 65 x...

Страница 31: ...ion 20 Frequency 1 Hz The maximum elongation achieved might only be 10 Reducing the frequency of the regimen to 0 5 Hz will produce an achievable elongation of 18 The maximum allowable elongation for...

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