F
LEXCELL
®
I
NTERNATIONAL
C
ORPORATION
4
are to be used simultaneously, connect the
baseplates using the configuration as shown in
Figure 6.
NOTE:
Please see the Appendix (pg. 25) for the
maximum allowable % elongations when using
two baseplates simultaneously.
Figure 5.
Single baseplate connection
Figure 6.
"T" connection for two baseplates
2.5 S
ETUP OF
B
IO
F
LEX
B
ASEPLATE
(
S
)
WITH
L
OADING
S
TATIONS
™
AND
C
ULTURE
P
LATES
Place the BioFlex
®
baseplate onto one of the
incubator shelves. Be sure the shelf is level
front to back and side to side in the incubator.
1.
Attach the
FLEX IN
and
FLEX OUT
quick disconnect fittings to the respective
fittings on the baseplate.
2.
If using Loading Stations
™
, place the
four sets of Loading Stations
™
into the
baseplate wells and apply silicone grease
to the tops of the posts (see the
Culture
Plate and Loading Stations
™
user’s
manual for detailed instructions).
3.
Place the 4 red gaskets onto the bases of
the four BioFlex
®
culture plates. Be sure
that the gaskets are fully pressed onto the
culture plates and that no parts are folded
under.
4.
Place the culture plates and gaskets into
the baseplate wells. Check to be sure that
the gaskets are not folded up at any point
of contact with the baseplate. If the four
gaskets feel loose within the plane of the
baseplate (can be easily moved right or
left, forward or backward without lifting
the gasket out of the well), they should
seal correctly.
5.
Place the Plexiglas
sheet with weights
on top (5-10 lbs) over the culture plates
to help seal the baseplate.
NOTE:
After a regimen is run for an hour or so,
there should be little or no moisture visible in the
lines or Water Traps. Condensation early on in
the experiment indicates an air leak. If
condensation is present, recheck the seals
around each of the culture plates.
2.6 C
ONNECTING THE
S
YSTEM
V
ACUUM
L
INES
Connect the blue
⅜
” (9.5 mm) vacuum inlet
tubing to a vacuum source. A vacuum pump
capable of producing a maximum vacuum of
-100 kPa and a flow rate of, at least, 5.7
ft
3
/min (161 L/min) is necessary for optimal
performance.
Keep the tubing length between the vacuum
source and FlexLink
®
as short as possible.
