flexcell FX-5000 Скачать руководство пользователя страница 16

 

F

LEXCELL

®

 

I

NTERNATIONAL 

C

ORPORATION

 

 

 

10

3.5

 

 C

REATING 

R

EGIMENS

 

 

NOTE:

  Please see the Appendix (pg. 25) for the 

maximum allowable % elongations when using 
two baseplates simultaneously.  

 
Before beginning, a regimen must be created 
for downloading. In the 

Regimen

 

menu, 

select 

Create new

. A new window will appear 

entitled 

Regimens

 with a list of the existing 

users and their regimens (Fig. 12). In the 

Existing Users

 box, click on the user name 

under which the regimen is to be created. A 
list of current regimens created under this user 
will appear in the 

Regimens for Selected 

User

 box, unless no regimens have been saved 

under this user, in which case, this box will be 
empty. 
 
To create a new regimen, click on 

Run 

Wizard

 in the bottom left hand corner. This 

option will bring up a step by step process of 
creating a new regimen. Alternatively, follow 
the sequence of steps below:  
 
1)  Type a regimen name in the box labeled 

Regimen Name

 
2)  Choose the desired waveform shape under 

Shape

 by clicking on the downward arrow 

and selecting an entry from the drop-down 
list. 

 

 

Figure 12.

  FX-5000

™ 

regimen editor

 

 

3) Enter the desired minimum and 

maximum % elongation for the plate 
membranes (0-30%) in the boxes under 

Min

 and 

Max

.  

 
4)  Enter the frequency for the waveform 

(0.01-5 Hz) in the box under 

Freq

 
5)  If a static regimen is required, enter the 

desired % elongation at which the 
membrane is to be held in the 

Max 

box. 

Enter 0 in the 

Min

 box, and 1.0 in the 

Freq

 box (

Min

 and 

Freq

 are irrelevant 

for static regimens). 

 
6)  If a duty cycle for the waveform is to be 

defined, enter the desired value in the 
box under 

DC %

. The duty cycle is the 

percent of time that the waveform shape 
remains on at the high (square wave) or 
rising (triangle) part of the waveform. 
Duty cycle only affects the triangle and 
square waves. 

 
7) Enter the number of times for the 

regimen to repeat (0-65,535) in the box 
under 

Cycles

. When moving out this 

box, the box underneath 

dd:hh:mm:ss

 

will update to the calculated regimen 
time according to the set parameters. The 
format for this entry is 
days:hours:minutes:seconds. However, 
if the time is entered first, the program 
will calculate the number of cycles. 

8)  To make a regimen step (except for the 

first step) return (jump back) to a 
previous regimen step, enter the return 
step number in the box under 

Back To

Then, type the number of times to repeat 
this process in the box under 

Repeat

.  

 
  For instance, if the user was entering 

Step 4 and wanted to return to Step 2 and 
repeat Steps 2 through 4 three times, 
enter 2 under 

Back To

 and 3 under 

Repeat 

when inputting Step 4.  

Содержание FX-5000

Страница 1: ...21 0 Culturing Cells in a Mechanically Active Environment Flexcell International Corporation 2730 Tucker Street Suite 200 Burlington NC 27215 800 728 3714 919 732 1591 FAX 919 732 5196 www flexcellin...

Страница 2: ...uctions 7 3 1 Starting the FX 5000 Program 7 3 2 Using the FX 5000 Tutorial 7 3 3 Main Display Window 7 3 4 Configuring Users 9 3 5 Creating Regimens 10 3 6 Creating a Regimen with a Custom Waveform S...

Страница 3: ...than our factory or distributor Breaking the seal and opening the unit case or violating unit components will void the warranty Failure to run the drying regimen as recommended in this manual will vo...

Страница 4: ...nty are listed below You will need to have the service tag number and Express Service Code number which are located on the side of the computer Dell Hardware Limited Warranty Plus Onsite Service Exten...

Страница 5: ...to a properly grounded power outlet and used with the power cord supplied with the unit s unmodified 3 The unit must be operated with the factory installed cover in place 4 No modifications of the equ...

Страница 6: ...seplate s in the incubator a Insert the 6 4 mm and 9 5 mm tubing into the access opening of the incubator b Connect the quick disconnects for the 6 4 mm and 9 5 mm tubing to the baseplate s c Place fo...

Страница 7: ...D for vacuum source connection 16 Flexcell FX 5000 Tension System user s manual and other ancillary manuals for computer and monitor 17 Surge protected outlet strip 18 Water Trap x2 19 Vacuum source o...

Страница 8: ...the system is 48 37 lbs 21 96 kg Figure 3 Dell OptiPlex rear view 2 3 SETUP OF THE COMPUTER AND FX5K TENSION FLEXLINK Setup the Dell OptiPlex desktop computer and peripherals according to the manufac...

Страница 9: ...onto the fitting Tighten each nut by hand until the tubing is crimped to minimize vacuum leakage Connect the power cables 115 VAC or 230 VAC to the rear power inlet FlexLink Power of the FlexLink Fig...

Страница 10: ...ure that the gaskets are fully pressed onto the culture plates and that no parts are folded under 4 Place the culture plates and gaskets into the baseplate wells Check to be sure that the gaskets are...

Страница 11: ...use the auto control pump feature plug the vacuum pump power cable into the PUMP CONTROL CONNECTION outlet on the rear of the FX5K Tension FlexLink Fig 2 and 7 NOTE When using the auto pump control fe...

Страница 12: ...imen Ready User name Platform Regimen Name Flexcell International Corp www flexcellint com Regimen is running or being simulated 1 Regimen Left 5 min User name Platform Regimen Name Flexcell Internati...

Страница 13: ...ength as much as possible between the vacuum source and the baseplate See page 16 for more information on obtaining the optimal performance of your FX 5000 Tension System 3 1 STARTING THE FX 5000 PROG...

Страница 14: ...al data patterns may be confounded with noise For the case of the pressure plot as a function of voltage from the FX 5000 Tension System pressure transducer the rough data yield a plot with a coeffici...

Страница 15: ...changed by clicking on one of the two small double arrow buttons at either end of the axis The button with arrows pointing away from each other increases the axis scale while the button with arrows po...

Страница 16: ...boxes under Min and Max 4 Enter the frequency for the waveform 0 01 5 Hz in the box under Freq 5 If a static regimen is required enter the desired elongation at which the membrane is to be held in the...

Страница 17: ...ed regimen name in the Regimens for Selected User box and then click on Delete Regimen Confirm the deletion by clicking on Yes NOTE If the regimen to be deleted is currently assigned the regimen must...

Страница 18: ...ll be MAXIMUM and the regimen cannot be saved Adjust the time and or cycle number to decrease the total duration time 7 Click on Save Regimen 8 When finished with regimen editing click on Close 3 7 AS...

Страница 19: ...unusual elements is to accommodate for the lag time during sudden changes in pressure e g rising falling edge of a square wave Without these unusual elements the valve controllers may oscillate for a...

Страница 20: ...l turn the pump ON The software will turn the pump OFF when a regimen is complete This option also allows the pump to be turned OFF during a regimen step when a static waveform regimen is selected tha...

Страница 21: ...box can be set so that the data viewed in the plot begin at the specified time entered here Use the timeline at the top of the graph to navigate through the regimen Users can move the slider function...

Страница 22: ...the incubator and stored flat to prevent warping Do not hang the red gaskets on pegs to dry They will stretch over time 3 18 EXITING THE FX 5000 PROGRAM To exit the FX 5000 software in the Controller...

Страница 23: ...elongation and frequency a Shorten the FLEX IN FLEX OUT and SYSTEM tubing to their minimum possible lengths See section 2 2 for minimum lengths b Use a more powerful vacuum source if recommended spec...

Страница 24: ...Regimen wave is flatlining Solution 2 Remove the SYSTEM vacuum line at the back of the FlexLink and check to be sure that there is vacuum coming through the line Check the VENT port at the back of the...

Страница 25: ...of the cells in the middle of the well due to gravity or b incomplete trypsinization or over trypsinization of cells causing clumping and uneven distribution of cells Solution 8 a Use 2 3 ml of media...

Страница 26: ...y dry before connecting to the FX5K Tension FlexLink Contact time time surface remains wet of the above solutions is critical to the effectiveness of disinfecting Times given are minimal and longer co...

Страница 27: ...re from min to max Triangle Wave This waveform provides a linear ramped rise to the max and a linear ramped fall back to the min Square Wave This waveform provides the fastest possible rise to max fol...

Страница 28: ...inusoidal wave Heart wave Electronic and Pressure Triangular wave Square wave and custom waveforms Provides equibiaxial strain or unconstrained distension to the BioFlex culture plate membrane Optiona...

Страница 29: ...m design vacuum controller board o Microprocessor sampling rate for display data transducer 200 Hz o Microprocessor sampling rate for feedback error valve adjustment transducer 1 KHz 2 proportional so...

Страница 30: ...equibiaxial strain Minimum achievable elongation with the FX 5000 1 2 Maximum achievable elongation with the FX 5000 8 0 FX 5000 TENSION PHYSICAL SPECIFICATIONS FX 5000 Computer Size W x H x D 3 65 x...

Страница 31: ...ion 20 Frequency 1 Hz The maximum elongation achieved might only be 10 Reducing the frequency of the regimen to 0 5 Hz will produce an achievable elongation of 18 The maximum allowable elongation for...

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