flexcell FX-5000 Скачать руководство пользователя страница 11

 

F

LEXCELL

®

 

I

NTERNATIONAL 

C

ORPORATION

 

 

 

5

Also, keep in mind that some vacuum systems 
will vary in flow rate and/or pressure level. To 
eliminate or reduce this effect, use of a 
pressure reservoir between the vacuum source 
and the FX5K

 Tension FlexLink

®

 is 

recommended. 
 
Flexcell

®

 International Corp. recommends the 

Leybold Trivac D8B Pump vacuum pump 
(includes the D8B pump system, power cable, 
gas ballast valve and AR 4-8 return filter) as a 
vacuum source as well as using a pressure 
reservoir. The vacuum pump and pressure 
reservoir can be purchased directly from 
Flexcell

®

 International Corp.  

 

NOTE:

  Even with an optimal vacuum source, 

there will still be some 

system limitations

 due to 

airflow. Combinations of high frequencies and % 
elongations may not be achievable due to the high 
vacuum level in the short period of time requested. 
An example would be a regimen programmed to 
run a sine wave from 0–20% elongation at 1.0 Hz, 
using the 25 mm BioFlex

®

 Loading Stations

. The 

system is not able to achieve the vacuum required 
to reach 20% in 0.5 seconds because of airflow 
limitations. The maximum % elongation may only 
reach 15%. The exact output is dependent on 
many system variables. See the Addendum on page 
16 for suggestions on maximizing system 
performance. 

 
To use the auto control pump feature, plug the 
vacuum pump power cable into the 

PUMP 

CONTROL CONNECTION

 outlet on the 

rear of the FX5K

 Tension FlexLink

®

 (Fig. 2 

and 7).   
 

NOTE:

 

When using the auto pump control 

feature, the power cable from the FX5K

 

Tension FlexLink

®

 must be connected directly 

into the mains power.  Failure to do so will cause 
damage to the FX5K

 Tension FlexLink

®

 
See page 14 for additional comments on 
setting up the vacuum power to use the auto 
control feature. 
 

 

Figure 7.

  Flexcell

®

 FX-5000

 Tension System 

power set-up with auto control pump feature 

 

2.7  F

LEX

S

OFT

®

 

FX-5000

 

S

OFTWARE 

AND 

S

YSTEM 

P

OWER

-

UP

 

 

The FlexSoft

®

 FX-5000

 software 

(Flexcell

®

 Copyright Protected Software, 

Property of Flexcell

®

 International 

Corporation, 2730 Tucker Street, Suite 
200, Burlington, NC 27215) has been pre-
installed on the hard drive.  

 

Keep the CD package included with your 
system and store it in a safe place. Be sure 
to shutdown Windows

 properly by using 

the 

Shut Down

 command activated by the 

Start

 button. Failure to follow the above 

directions can cause software and 
hardware malfunctions. 

 
The computer has Windows

 7 installed on 

the hard drive. All backup software is 
provided with the system. Check all 
connections and connect the surge protector 
into an appropriate power outlet (115 VAC 
or 230 VAC). Turn ON the surge protector 
strip and check that the indicator light on the 
strip is lit. If the power light does not come 
on, the surge protector has failed. Replace 

Содержание FX-5000

Страница 1: ...21 0 Culturing Cells in a Mechanically Active Environment Flexcell International Corporation 2730 Tucker Street Suite 200 Burlington NC 27215 800 728 3714 919 732 1591 FAX 919 732 5196 www flexcellin...

Страница 2: ...uctions 7 3 1 Starting the FX 5000 Program 7 3 2 Using the FX 5000 Tutorial 7 3 3 Main Display Window 7 3 4 Configuring Users 9 3 5 Creating Regimens 10 3 6 Creating a Regimen with a Custom Waveform S...

Страница 3: ...than our factory or distributor Breaking the seal and opening the unit case or violating unit components will void the warranty Failure to run the drying regimen as recommended in this manual will vo...

Страница 4: ...nty are listed below You will need to have the service tag number and Express Service Code number which are located on the side of the computer Dell Hardware Limited Warranty Plus Onsite Service Exten...

Страница 5: ...to a properly grounded power outlet and used with the power cord supplied with the unit s unmodified 3 The unit must be operated with the factory installed cover in place 4 No modifications of the equ...

Страница 6: ...seplate s in the incubator a Insert the 6 4 mm and 9 5 mm tubing into the access opening of the incubator b Connect the quick disconnects for the 6 4 mm and 9 5 mm tubing to the baseplate s c Place fo...

Страница 7: ...D for vacuum source connection 16 Flexcell FX 5000 Tension System user s manual and other ancillary manuals for computer and monitor 17 Surge protected outlet strip 18 Water Trap x2 19 Vacuum source o...

Страница 8: ...the system is 48 37 lbs 21 96 kg Figure 3 Dell OptiPlex rear view 2 3 SETUP OF THE COMPUTER AND FX5K TENSION FLEXLINK Setup the Dell OptiPlex desktop computer and peripherals according to the manufac...

Страница 9: ...onto the fitting Tighten each nut by hand until the tubing is crimped to minimize vacuum leakage Connect the power cables 115 VAC or 230 VAC to the rear power inlet FlexLink Power of the FlexLink Fig...

Страница 10: ...ure that the gaskets are fully pressed onto the culture plates and that no parts are folded under 4 Place the culture plates and gaskets into the baseplate wells Check to be sure that the gaskets are...

Страница 11: ...use the auto control pump feature plug the vacuum pump power cable into the PUMP CONTROL CONNECTION outlet on the rear of the FX5K Tension FlexLink Fig 2 and 7 NOTE When using the auto pump control fe...

Страница 12: ...imen Ready User name Platform Regimen Name Flexcell International Corp www flexcellint com Regimen is running or being simulated 1 Regimen Left 5 min User name Platform Regimen Name Flexcell Internati...

Страница 13: ...ength as much as possible between the vacuum source and the baseplate See page 16 for more information on obtaining the optimal performance of your FX 5000 Tension System 3 1 STARTING THE FX 5000 PROG...

Страница 14: ...al data patterns may be confounded with noise For the case of the pressure plot as a function of voltage from the FX 5000 Tension System pressure transducer the rough data yield a plot with a coeffici...

Страница 15: ...changed by clicking on one of the two small double arrow buttons at either end of the axis The button with arrows pointing away from each other increases the axis scale while the button with arrows po...

Страница 16: ...boxes under Min and Max 4 Enter the frequency for the waveform 0 01 5 Hz in the box under Freq 5 If a static regimen is required enter the desired elongation at which the membrane is to be held in the...

Страница 17: ...ed regimen name in the Regimens for Selected User box and then click on Delete Regimen Confirm the deletion by clicking on Yes NOTE If the regimen to be deleted is currently assigned the regimen must...

Страница 18: ...ll be MAXIMUM and the regimen cannot be saved Adjust the time and or cycle number to decrease the total duration time 7 Click on Save Regimen 8 When finished with regimen editing click on Close 3 7 AS...

Страница 19: ...unusual elements is to accommodate for the lag time during sudden changes in pressure e g rising falling edge of a square wave Without these unusual elements the valve controllers may oscillate for a...

Страница 20: ...l turn the pump ON The software will turn the pump OFF when a regimen is complete This option also allows the pump to be turned OFF during a regimen step when a static waveform regimen is selected tha...

Страница 21: ...box can be set so that the data viewed in the plot begin at the specified time entered here Use the timeline at the top of the graph to navigate through the regimen Users can move the slider function...

Страница 22: ...the incubator and stored flat to prevent warping Do not hang the red gaskets on pegs to dry They will stretch over time 3 18 EXITING THE FX 5000 PROGRAM To exit the FX 5000 software in the Controller...

Страница 23: ...elongation and frequency a Shorten the FLEX IN FLEX OUT and SYSTEM tubing to their minimum possible lengths See section 2 2 for minimum lengths b Use a more powerful vacuum source if recommended spec...

Страница 24: ...Regimen wave is flatlining Solution 2 Remove the SYSTEM vacuum line at the back of the FlexLink and check to be sure that there is vacuum coming through the line Check the VENT port at the back of the...

Страница 25: ...of the cells in the middle of the well due to gravity or b incomplete trypsinization or over trypsinization of cells causing clumping and uneven distribution of cells Solution 8 a Use 2 3 ml of media...

Страница 26: ...y dry before connecting to the FX5K Tension FlexLink Contact time time surface remains wet of the above solutions is critical to the effectiveness of disinfecting Times given are minimal and longer co...

Страница 27: ...re from min to max Triangle Wave This waveform provides a linear ramped rise to the max and a linear ramped fall back to the min Square Wave This waveform provides the fastest possible rise to max fol...

Страница 28: ...inusoidal wave Heart wave Electronic and Pressure Triangular wave Square wave and custom waveforms Provides equibiaxial strain or unconstrained distension to the BioFlex culture plate membrane Optiona...

Страница 29: ...m design vacuum controller board o Microprocessor sampling rate for display data transducer 200 Hz o Microprocessor sampling rate for feedback error valve adjustment transducer 1 KHz 2 proportional so...

Страница 30: ...equibiaxial strain Minimum achievable elongation with the FX 5000 1 2 Maximum achievable elongation with the FX 5000 8 0 FX 5000 TENSION PHYSICAL SPECIFICATIONS FX 5000 Computer Size W x H x D 3 65 x...

Страница 31: ...ion 20 Frequency 1 Hz The maximum elongation achieved might only be 10 Reducing the frequency of the regimen to 0 5 Hz will produce an achievable elongation of 18 The maximum allowable elongation for...

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