EMTEK, LLC - R100 USERS MANUAL / AUG2019.REV01
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Appendix A: General Sampling with the R100
PURPOSE
To describe the procedure to monitor for viable airborne bacteria with the R100 Remote Microbial Air Sampler
from EMTEK, LLC.
A.1 R100 Operating Principles
A 90 mm agar-based test plate is placed on the adjustable stage of the externally attached R100 Remote Air Sampler
(RAS), underneath the inlet cover assembly. The required plate height is set with the aid of the adjustable stage and
media distance indicator on the inside top of the inlet lid. The required sample volume or time period is set on the
R100 set up screen and the testing period is initiated. During testing, the vacuum source of the R100 draws 28.3
LPM (1 CFM), or 100 LPM into the R100 through the RAS’s sample inlet. Airborne bacteria in the sampled
volume of air become impinged (captured) on the test plate. The sampled air volume is then drawn through CPU
controlled flow system. The sampled air volume then passes through a 0.2 micron HEPA filter, as is exhausted at
the rear of the unit. An additional option allows for attachment of remote exhaust tubing to move the exhaust well
away from the test location. Upon completion of the test period, the test plate is removed from the R100 RAS and
is incubated for a designated time period at a specified temperature. Following the required incubation period, the
number of bacterial Colony Forming Units (CFU) are enumerated. The density of air borne bacteria per volume of
air tested can then determined.